TFB2M (NM_022366) Human Tagged ORF Clone

CAT#: RC201683

TFB2M (Myc-DDK-tagged)-Human transcription factor B2, mitochondrial (TFB2M), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC201683

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  Western blot validation of overexpression lysate (Cat# LY402922) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201683 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TFB2M protein (Cat# TP301683). The protein was produced from HEK293T cells transfected with TFB2M cDNA clone (Cat# RC201683) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TFB2M
• Synonyms: Hkp1; mtTFB2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201683 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGATCCCAGTGGTCGGGCTTCCTCGGCGGCTGAGGCTCTCCGCCTTGGCGGGCGCTGGTCGCTTTT
  GCATTTTAGGGTCTGAAGCGGCGACGCGAAAGCATTTGCCGGCGAGGAACCACTGTGGGCTCTCTGACTC
  CTCTCCGCAGCTGTGGCCCGAACCGGATTTCAGGAATCCGCCAAGGAAGGCGTCTAAGGCCAGCTTAGAC
  TTTAAGCGTTACGTAACCGATCGGAGATTGGCTGAGACCCTGGCGCAAATCTATTTGGGAAAACCAAGTA
  GACCTCCACACCTACTGCTGGAGTGCAATCCAGGTCCTGGAATCCTGACTCAGGCATTACTTGAAGCTGG
  TGCCAAAGTGGTTGCGCTCGAAAGTGACAAAACTTTTATTCCACATTTGGAGTCCTTAGGAAAAAATCTG
  GATGGAAAACTACGAGTGATTCACTGTGACTTCTTTAAACTAGATCCTAGAAGTGGTGGAGTAATAAAAC
  CACCTGCTATGTCTTCTCGAGGGCTCTTTAAGAATTTGGGAATAGAAGCAGTTCCTTGGACAGCAGACAT
  CCCTTTAAAAGTAGTTGGAATGTTCCCAAGTAGAGGTGAGAAAAGGGCACTTTGGAAACTCGCATATGAC
  TTGTATTCCTGTACTTCTATATATAAATTTGGACGAATAGAAGTAAATATGTTTATTGGTGAAAAAGAAT
  TCCAGAAACTAATGGCAGATCCTGGAAATCCAGACTTGTATCATGTATTAAGTGTTATCTGGCAATTAGC
  TTGTGAGATTAAGGTTCTGCACATGGAGCCTTGGTCATCATTTGATATATACACCCGGAAAGGGCCGCTG
  GAAAACCCAAAGCGTAGGGAATTATTAGACCAATTACAACAAAAGCTGTATCTTATTCAAATGATTCCTC
  GTCAAAATTTATTTACCAAGAACTTAACACCTATGAACTATAATATATTTTTTCACTTGTTAAAGCACTG
  TTTTGGGAGGCGCAGCGCCACTGTAATAGACCACTTACGTTCATTGACTCCACTTGATGCGAGAGATATA
  TTGATGCAAATAGGAAAACAGGAGGATGAGAAAGTAGTTAACATGCACCCTCAAGACTTCAAAACACTTT
  TTGAAACTATAGAGCGTTCCAAAGATTGTGCTTATAAATGGCTGTATGATGAAACCCTGGAAGATAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201683 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWIPVVGLPRRLRLSALAGAGRFCILGSEAATRKHLPARNHCGLSDSSPQLWPEPDFRNPPRKASKASLD
  FKRYVTDRRLAETLAQIYLGKPSRPPHLLLECNPGPGILTQALLEAGAKVVALESDKTFIPHLESLGKNL
  DGKLRVIHCDFFKLDPRSGGVIKPPAMSSRGLFKNLGIEAVPWTADIPLKVVGMFPSRGEKRALWKLAYD
  LYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPL
  ENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDI
  LMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022366
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022366.3
• RefSeq Size: 1799 bp
• RefSeq ORF: 1191 bp
• Locus ID: 64216
• UniProt ID: Q9H5Q4
• Cytogenetics: 1q44
• Domains: rADc
• Protein Families: Transcription Factors
• MW: 45.3 kDa
• Gene Summary: S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.[UniProtKB/Swiss-Prot Function]