EIF2S2 (NM_003908) Human Tagged ORF Clone
CAT#: RC201563
EIF2S2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_003908" in other vectors (6)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | EIF2S2 |
Synonyms | eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201563 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAGAAGCCTTTTA TGTTAGATGAGGAAGGGGATACCCAAACAGAGGAAACCCAGCCTTCAGAAACAAAAGAAGTGGAGCCAGA GCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGACACTAGGAAAAAAGATGCTTCTGATGATCTA GATGACTTGAACTTCTTTAATCAAAAGAAAAAGAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAG CTGAAGAAGGTGTAAAGGATCTTAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAGAGGATGACCT TGACATTATGCTTGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCCCAGATGAGGATGAAATACTA GAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGTAATCAGACAG GCCCTGCTTGGGCAGGCTCAGAAAGAGACTACACATACGATGAGCTGCTGAATCGAGTGTTCAACATCAT GAGGGAAAAGAATCCAGATATGGTTGCTGGGGAGAAAAGGAAATTTGTCATGAAACCTCCACAAGTCGTC CGAGTAGGAACCAAGAAAACTTCTTTTGTCAACTTTACAGATATCTGTAAACTATTACATCGTCAGCCCA AACATCTCCTTGCATTTTTGTTGGCTGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGT AATCAAAGGAAGATTCCAACAGAAACAGATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACT TGTCACACATGCCGATCACCGGACACAATCCTGCAGAAGGACACACGACTCTATTTCCTACAGTGCGAAA CTTGTCATTCTAGATGTTCTGTTGCCAGTATCAAAACCGGCTTCCAGGCTGTCACGGGCAAGCGAGCACA GCTCCGTGCCAAAGCTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201563 protein sequence
Red=Cloning site Green=Tags(s) MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDL DDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEIL EKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYDELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVV RVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVT CHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003908 |
ORF Size | 999 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003908.5 |
RefSeq Size | 2592 bp |
RefSeq ORF | 1002 bp |
Locus ID | 8894 |
UniProt ID | P20042 |
Cytogenetics | 20q11.22 |
Domains | eIF2B_5 |
MW | 38.4 kDa |
Gene Summary | Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201563L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), Myc-DDK-tagged |
USD 600.00 |
|
RC201563L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mGFP tagged |
USD 600.00 |
|
RC201563L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), Myc-DDK-tagged |
USD 600.00 |
|
RC201563L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2), mGFP tagged |
USD 600.00 |
|
RG201563 | EIF2S2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2) |
USD 500.00 |
|
SC117677 | EIF2S2 (untagged)-Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2) |
USD 457.00 |
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