EIF2S2 (NM_003908) Human Untagged Clone

CAT#: SC117677

EIF2S2 (untagged)-Human eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa (EIF2S2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF2S2
• Synonyms: eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003908 edited
  GAATTCGGCACGAGGCTTTCGCTGATGCAAGAGCCTAGTGCGGTGGTGGGAGAGGTATCG
  GCAGGGGCAGCGCTGCCGCCGGGGCCTGGGGCTGACCCGTCTGACTTCCCGTCCGTGCCG
  AGCCCACTCGAGCCGCAGCCATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCA
  AGAAGAAAAAGAAGAAGAAGAAGCCTTTTATGTTAGATGAGGAAGGGGATACCCAAACAG
  AGGAAACCCAGCCTTCAGAAACAAAAGAAGTGGAGCCAGAGCCAACTGAGGACAAGGATT
  TGGAAGCTGATGAAGAGGACACTAGGAAAAAAGATGCTTCTGATGATCTAGATGACTTGA
  ACTTCTTTAATCAAAAGAAAAAGAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAG
  CTGAAGAAGGTGTAAAGGATCTTAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAG
  AGGATGACCTTGACATTATGCTTGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCC
  CAGATGAGGATGAAATACTAGAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAG
  ATGATGGTATCTCATTCAGTAATCAGACAGGCCCTGCTTGGGCAGGCTCAGAAAGAGACT
  ACACATACGAGGAGCTGCTGAATCGAGTGTTCAACATCATGAGGGAAAAGAATCCAGATA
  TGGTTGCTGGGGAGAAAAGGAAATTTGTCATGAAACCTCCACAAGTCGTCCGAGTAGGAA
  CCAAGAAAACTTCTTTTGTCAACTTTACAGATATCTGTAAACTATTACATCGTCAGCCCA
  AACATCTCCTTGCATTTTTGTTGGCTGAATTGGGTACAAGTGGTTCTATAGATGGTAATA
  ACCAACTTGTAATCAAAGGAAGATTCCAACAGAAACAGATAGAAAATGTCTTGAGAAGAT
  ATATCAAGGAATATGTCACTTGTCACACATGCCGATCACCGGACACAATCCTGCAGAAGG
  ACACACGACTCTATTTCCTACAGTGCGAAACTTGTCATTCTAGATGTTCTGTTGCCAGTA
  TCAAAACCGGCTTCCAGGCTGTCACGGGCAAGCGAGCACAGCTCCGTGCCAAAGCTAACT
  AATTTGCTAATCACTGATTTTGCAAAGCTTGTTGTGGAGATGTGGCTGGACAGGTTTGCC
  ATCAGAGTGGATATACCGTTGTATTAAAAACAAGATAAAAAAGCTGCCAAGATTTTTGGC
  GAGTGGTTGGTCTGAAGTCCTTGCAAGACGCTGATGCTCAAGCTGTTGACATACTCATTG
  CCTACTTTAACACCTGTCAGAGAAACGTGATATGGGGTAAGGAGGTGCTTTTTTAAAATC
  GTTCATAGACTTCTGTAAAATGCAAGATAAATTAAAGTTATTATAACAGTGAAAAAAAAA
  AAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003908 unedited
  GGCTTTAGNATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCTTTCGC
  TGATGCAAGAGCCTAGTGCGGTGGTGGGAGAGGTATCGGCAGGGGCAGCGCTGCCGCCGG
  GGCCTGGGGCTGACCCGTCTGACTTCCCGTCCGTGCCGAGCCCACTCGAGCCGCAGCCAT
  GTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAGAA
  GCCTTTTATGTTAGATGAGGAAGGGGATACCCAAACAGAGGAAACCCAGCCTTCAGAAAC
  AAAAGAAGTGGAGCCAGAGCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGACAC
  TAGGAAAAAAGATGCTTCTGATGATCTAGATGACTTGAACTTCTTTAATCAAAAGAAAAA
  GAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAGCTGAAGAAGGTGTAAAGGATCT
  TAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAGAGGATGACCTTGACATTATGCT
  TGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCCCAGATGAGGATGAAATACTAGA
  GAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGTAA
  TCAGACAGGCCCTGCTTGGGCAGGCTCAGAAAGAGACTACACATACGAGGAGCTGCTGAA
  TCGAGTGTTCAACATCATGAGGGAAAAGAATCCAGATATGGTTGCTGGGGAGAAAAGAAA
  TTTGTCATGAAACCTCCACAGTCGTCCGAGTAGGAACCAAGANAACTTCTTTTGTNCACT
  NTACAGATATCTGGTAACTATTACATCGTCAGCNCAAACATCTCCTTGCATTNTTGTTGG
  CTGAATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003908 unedited
  CCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCACTGTTATAATAACTTTAATTT
  ATCTTGCATTTTACAGAAGTCTATGAACGATTTTAAAAAAGCACCTCCTTACCCCATATC
  ACGTTTCTCTGACAGGTGTTAAAGTAGGCAATGAGTATGTCAACAGCTTGAGCATCAGCG
  TCTTGCAAGGACTTCAGACCAACCACTCGCCAAAAATCTTGGCAGCTTTTTTATCTTGTT
  TTTAATACAACGGTATATCCACTCTGATGGCAAACCTGTCCAGCCACATCTCCACAACAA
  GCTTTGCAAAATCAGTGATTAGCAAATTAGTTAGCTTTGGCACGGAGCTGTGCTCGCTTG
  CCCGTGACAGCCTGGAAGCCGGTTTTGATACTGGCAACAGAACATCTAGAATGACAAGTT
  TCGCACTGTAGGAAATAGAGTCGTGTGTCCTTCTGCAGGATTGTGTCCGGTGATCGGCAT
  GTGTGACAAGTGACATATTCCTTGATATATCTTCTCAAGACATTTTCTATCTGTTTCTGT
  TGGAATCTTCCTTTGATTACAAGTTGGTTATTACCATCTATAGAACCACTTGTACCCAAT
  TCAGCCAACAAAAATGCAAGGAGATGTTTGGGCTGACGATGTAATAGTTTACAGATATCT
  GTAAAGTTGACAAAAGAAGTTTTCTTGGTTCCTACTCGGACGACTTGTGGAGGTTTCATG
  ACAAATTTCCTTTTCTCCCCACAACCATATCTGAATTCTTTTCCCTCATGATGTTGAACA
  CTCCGATCAGCAGCCTCTCGTATGTGTAGTCTCTTTTTGAGCCTGCCCAAGCAGGGCCTG
  TCTGATTACTGAATGAGAAACCATCATCCTTTTTTGTTGCCTTCATTCTCTAGAGCTNCA
  CCTTCTCTAGACTTCAACCTCATCTGGGAACCTAACATTCTTCTTTTCTTCTTTTATTGC
  CAGCATATGCAGGNCATCCTCTGGTTAATTGGTCCTAGACTCACTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003908
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003908.2, NP_003899.2
• RefSeq Size: 1439 bp
• RefSeq ORF: 1002 bp
• Locus ID: 8894
• UniProt ID: P20042
• Cytogenetics: 20q11.22
• Domains: eIF2B_5
• Gene Summary: Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.