BRP44L (MPC1) (NM_016098) Human Tagged ORF Clone
MPC1 (Myc-DDK-tagged)-Human brain protein 44-like (BRP44L)
|Type||Human Tagged ORF Clone|
|Synonyms||BRP44L; CGI-129; MPYCD; SLC54A1|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC201461 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201461 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||327 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||977 bp|
|RefSeq ORF||330 bp|
|Gene Summary||The protein encoded by this gene is part of an MPC1/MPC2 heterodimer that is responsible for transporting pyruvate into mitochondria. The encoded protein is found in the inner mitochondrial membrane. Defects in this gene are a cause of mitochondrial pyruvate carrier deficiency. Several transcript variants, some protein coding and one non-protein coding, have been found for this gene. [provided by RefSeq, Aug 2012]|
|cDNA Clone Resources|
|RC201461L3||Lenti ORF clone of Human brain protein 44-like (BRP44L), Myc-DDK-tagged||
|RC201461L4||Lenti ORF clone of Human brain protein 44-like (BRP44L), mGFP tagged||
|RG201461||MPC1 (GFP-tagged) - Human brain protein 44-like (BRP44L)||
|SC127044||MPC1 (untagged)-Human brain protein 44-like (BRP44L)||