RAI3 (GPRC5A) (NM_003979) Human Tagged ORF Clone
GPRC5A (Myc-DDK-tagged)-Human G protein-coupled receptor, family C, group 5, member A (GPRC5A)
|Type||Human Tagged ORF Clone|
|Synonyms||GPCR5A; PEIG-1; RAI3; RAIG1; TIG1|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC200118 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200118 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1071 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2856 bp|
|RefSeq ORF||1074 bp|
|Protein Families||Druggable Genome, GPCR, Transmembrane|
|Gene Summary||This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC200118L1||Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member A (GPRC5A), Myc-DDK-tagged||
|RC200118L2||Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member A (GPRC5A), mGFP tagged||
|RC200118L3||Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member A (GPRC5A), Myc-DDK-tagged||
|RC200118L4||Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member A (GPRC5A), mGFP tagged||
|RG200118||GPRC5A (tGFP-tagged) - Human G protein-coupled receptor, family C, group 5, member A (GPRC5A)||
|SC117639||GPRC5A (untagged)-Human G protein-coupled receptor, family C, group 5, member A (GPRC5A)||