RAI3 (GPRC5A) (NM_003979) Human Untagged Clone

CAT#: SC117639

GPRC5A (untagged)-Human G protein-coupled receptor, family C, group 5, member A (GPRC5A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAI3
• Synonyms: GPCR5A; PEIG-1; RAI3; RAIG1; TIG1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117639 sequence for NM_003979 edited (data generated by NextGen Sequencing)
  ATGGCTACAACAGTCCCTGATGGTTGCCGCAATGGCCTGAAATCCAAGTACTACAGACTT
  TGTGATAAGGCTGAAGCTTGGGGCATCGTCCTAGAAACGGTGGCCACAGCCGGGGTTGTG
  ACCTCGGTGGCCTTCATGCTCACTCTCCCGATCCTCGTCTGCAAGGTGCAGGACTCCAAC
  AGGCGAAAAATGCTGCCTACTCAGTTTCTCTTCCTCCTGGGTGTGTTGGGCATCTTTGGC
  CTCACCTTCGCCTTCATCATCGGACTGGACGGGAGCACAGGGCCCACACGCTTCTTCCTC
  TTTGGGATCCTCTTTTCCATCTGCTTCTCCTGCCTGCTGGCTCATGCTGTCAGTCTGACC
  AAGCTCGTCCGGGGGAGGAAGCCCCTTTCCCTGTTGGTGATTCTGGGTCTGGCCGTGGGC
  TTCAGCCTAGTCCAGGATGTTATCGCTATTGAATATATTGTCCTGACCATGAATAGGACC
  AACGTCAATGTCTTTTCTGAGCTTTCCGCTCCTCGTCGCAATGAAGACTTTGTCCTCCTG
  CTCACCTACGTCCTCTTCTTGATGGCGCTGACCTTCCTCATGTCCTCCTTCACCTTCTGT
  GGTTCCTTCACGGGCTGGAAGAGACATGGGGCCCACATCTACCTCACGATGCTCCTCTCC
  ATTGCCATCTGGGTGGCCTGGATCACCCTGCTCATGCTTCCTGACTTTGACCGCAGGTGG
  GATGACACCATCCTCAGCTCCGCCTTGGCTGCCAATGGCTGGGTGTTCCTGTTGGCTTAT
  GTTAGTCCCGAGTTTTGGCTGCTCACAAAGCAACGAAACCCCATGGATTATCCTGTTGAG
  GATGCTTTCTGTAAACCTCAACTCGTGAAGAAGAGCTATGGTGTGGAGAACAGAGCCTAC
  TCTCAAGAGGAAATCACTCAAGGTTTTGAAGAGACAGGGGACACGCTCTATGCCCCCTAT
  TCCACACATTTTCAGCTGCAGAACCAGCCTCCCCAAAAGGAATTCTCCATCCCACGGGCC
  CACGCTTGGCCGAGCCCTTACAAAGACTATGAAGTAAAGAAAGAGGGCAGCTAA
  
  Clone variation with respect to NM_003979.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003979 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCAGCACTGAGGAGCTCGC
  CTGCTGCCCTCTTGCGCGCGGGAAGCAGCACCAAGTTCACGGCCAACGCCTTGGCACTAG
  GGTCCAGAATGGCTACAACAGTCCCTGATGGTTGCCGCAATGGCCTGAAATCCAAGTACT
  ACAGACTTTGTGATAAGGCTGAAGCTTGGGGCATCGTCCTAGAAACGGTGGCCACAGCCG
  GGGTTGTGACCTCGGTGGCCTTCATGCTCACTCTCCCGATCCTCGTCTGCAAGGTGCAGG
  ACTCCAACAGGCGAAAAATGCTGCCTACTCAGTTTCTCTTCCTCCTGGGTGTGTTGGGCA
  TCTTTGGCCTCACCTTCGCCTTCATCATCGGACTGGACGGGAGCACAGGGCCCACACGCT
  TCTTCCTCTTTGGGATCCTCTTTTCCATCTGCTTCTCCTGCCTGCTGGCTCATGCTGTCA
  GTCTGACCAAGCTCGTCCGGNGGAGGAAGCCCCTTTCCCTGTTGGTGATTCTGGGTCTGG
  CCGTGGGCTTCAGCCTAGTCCAGGATGTTATCGCTATTGATATATTGTCCTGACCATGAA
  TAGGACCAACGTCAATGTCTTTTCTGAGCTTTCCGCTCCTCGTCGCAATGAAGACTTTGT
  CCTCCTGCTCACCTACGTNCTCTTCTTGATGGCGCTGACCTTNCTCATGTCCTCCTTCAC
  CTTCTGTGGNTCCTTACCGGCTGGAAGAACATGGGGCCACATTTACTNACGATGCNTCCC
  TCATTGCATCTGGTGGCCTGGACACCCTGCTATGCTTCTGACTTGACCGCAGTGGATGAC
  CCATCTCANCTCGCCTTGGTGCATGGCTGGGGTCCTGTTGCTATGTACNCCCAATTTGCT
  GCTACAACAACAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003979 unedited
  ACCATGGAAGTATGGCCACCACATCTTTATTGCATACTCAGGTGAATAACTTATTATACA
  ATGAACACTCCTCCATTAGGAGACCATGCCCACTTACAGAATGCAGCCGTAAATGCGGTA
  AATCTATTTACAGAGGTTGGGGTGCAAGATGAGAGAAGTATCAGCCCCAGGAATTTGAAG
  TGAGAATGATCTACAAATTCTCCTGACAAGGAGCAACCGGGCTTGTGCTAGTGAGGTCTG
  AAAGAATTCCTGGCAGAGCGTAGGGGGAGATTAGATCTCGGAATTGACAGCAAGTTTGGG
  GACAGTGCAAGAAGAGAGGGGTGACCTGTGAATTGGTGCTGGGGAGCTGCTGAGGCCCAA
  TGTGAGGCAGCACTAGAGAGATGAGTAAATTTAGGGTGATCTTTAGCCTCTCCTACCCAG
  GCAAGAAAGGTTGGGGAGCGGGGGTGCCANCAAGTTGGCTTCCAGTGTAGATTCAGGCCA
  GTGGGCTGAGAGAGGCTTTTGCTATTTTTGCTCAGATATGCANGTCTGCTCTGGGCCACA
  GAGCCTCACCAAACCCACTGTGAGTTAGAGGAGCAGGCCCCTANAGCTGTGTGAGCCAGC
  GTGAGCTCANATGACCAGACCCTTAGTCCATGGAAAATACAGCCAGATTAGGGCCTANGC
  TGGGAGCTGTGGCTCACGCCTGTCATCCCAACACTTTGGGAGATGGAGATGGGAAGTATG
  CTTTGACCCCAGGTGTTAAGGGTGCACTGGGCTGGATCCGCCCCTGGCCTTAACCCTGGT
  GACAGACAAGATCCTGTTTAAAAAACAACAAAAGAGTTAGGGCTTAACTTGGTTGCCTTG
  GCCTCCACTTAAAGAATCCTCCAGGGTTGACCCCAACAAAAAATTCTCCCCGCTCTTAAA
  CGCGGGGTTTTCTACCCACTTATTCCACCTGTGGAAATTGCGCCCCGCCTGGAGGGGCAA
  CCGCCCCCTGGGTCGTCCGACTCTATAAAACATAATACCCACCTCTTTGCTCCCCTTACC
  GCCCCGTCTTTTCCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003979
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003979.3, NP_003970.1
• RefSeq Size: 2856 bp
• RefSeq ORF: 1074 bp
• Locus ID: 9052
• UniProt ID: Q8NFJ5
• Cytogenetics: 12p13.1
• Domains: 7tm_3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]