YTHDF2 (NM_016258) Human Tagged ORF Clone

CAT#: RC200038

YTHDF2 (Myc-DDK-tagged)-Human YTH domain family, member 2 (YTHDF2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414098) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200038 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: YTHDF2
• Synonyms: CAHL; DF2; HGRG8; NY-REN-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200038 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCAGCAGCCTCTTGGAGCAGAGACCAAAAGGTCAAGGAAACAAAGTACAAAATGGATCTGTAC
  ATCAAAAGGATGGATTAAACGATGATGATTTTGAACCTTACTTGAGTCCACAGGCAAGGCCCAATAATGC
  ATATACTGCCATGTCAGATTCCTACTTACCCAGTTACTACAGTCCCTCCATTGGCTTCTCCTATTCTTTG
  GGTGAAGCTGCTTGGTCTACGGGGGGTGACACAGCCATGCCCTACTTAACTTCTTATGGACAGCTGAGCA
  ACGGAGAGCCCCACTTCCTACCAGATGCAATGTTTGGGCAACCAGGAGCCCTAGGTAGCACTCCATTTCT
  TGGTCAGCATGGTTTTAATTTCTTTCCCAGTGGGATTGACTTCTCAGCATGGGGAAATAACAGTTCTCAG
  GGACAGTCTACTCAGAGCTCTGGATATAGTAGCAATTATGCTTATGCACCTAGCTCCTTAGGTGGAGCCA
  TGATTGATGGACAGTCAGCTTTTGCCAATGAGACCCTCAATAAGGCTCCTGGCATGAATACTATAGACCA
  AGGGATGGCAGCACTGAAGTTGGGTAGCACAGAAGTTGCAAGCAATGTTCCAAAAGTTGTAGGTTCTGCT
  GTTGGTAGCGGGTCCATTACTAGTAACATCGTGGCTTCCAATAGTTTGCCTCCAGCCACCATTGCTCCTC
  CAAAACCAGCATCTTGGGCTGATATTGCTAGCAAGCCTGCAAAACAGCAACCTAAACTGAAGACCAAGAA
  TGGCATTGCAGGGTCAAGTCTTCCGCCACCCCCGATAAAGCATAACATGGATATTGGAACTTGGGATAAC
  AAGGGTCCCGTTGCAAAAGCCCCCTCACAGGCTTTGGTTCAGAATATAGGTCAGCCAACCCAGGGGTCTC
  CTCAGCCTGTAGGTCAGCAGGCTAACAATAGCCCACCAGTGGCTCAGGCATCAGTAGGGCAACAGACACA
  GCCATTGCCTCCACCTCCACCACAGCCTGCCCAGCTTTCAGTCCAGCAACAGGCAGCTCAGCCAACCCGC
  TGGGTAGCACCTCGGAACCGTGGCAGTGGGTTCGGTCATAATGGGGTGGATGGTAATGGAGTAGGACAGT
  CTCAGGCTGGTTCTGGATCTACTCCTTCAGAACCCCACCCAGTGTTGGAGAAGCTTCGGTCCATTAATAA
  CTATAACCCCAAAGATTTTGACTGGAATCTGAAACATGGCCGGGTTTTCATCATTAAGAGCTACTCTGAG
  GACGATATTCACCGTTCCATTAAGTATAATATTTGGTGCAGCACAGAGCATGGTAACAAGAGACTGGATG
  CTGCTTATCGTTCCATGAACGGGAAAGGCCCCGTTTACTTACTTTTCAGTGTCAACGGCAGTGGACACTT
  CTGTGGCGTGGCAGAAATGAAATCTGCTGTGGACTACAACACATGTGCAGGTGTGTGGTCCCAGGACAAA
  TGGAAGGGTCGTTTTGATGTCAGGTGGATTTTTGTGAAGGACGTTCCCAATAGCCAACTGCGACACATTC
  GCCTAGAGAACAACGAGAATAAACCAGTGACCAACTCTAGGGACACTCAGGAAGTGCCTCTGGAAAAGGC
  TAAGCAGGTGTTGAAAATTATAGCCAGCTACAAGCACACCACTTCCATTTTTGATGACTTCTCACACTAT
  GAGAAACGCCAAGAGGAAGAAGAAAGTGTTAAAAAGGAACGTCAAGGTCGTGGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200038 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYYSPSIGFSYSL
  GEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQ
  GQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSA
  VGSGSITSNIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDN
  KGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTR
  WVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSE
  DDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDK
  WKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHY
  EKRQEEEESVKKERQGRGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016258
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016258.3
• RefSeq Size: 3073 bp
• RefSeq ORF: 1740 bp
• Locus ID: 51441
• UniProt ID: Q9Y5A9
• Cytogenetics: 1p35.3
• Domains: YTH
• MW: 62.3 kDa
• Gene Summary: This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012]