LCMT1 (NM_016309) Human Tagged ORF Clone

CAT#: RC200018

LCMT1 (Myc-DDK-tagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1

Product Images

Circular map for RC200018

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LCMT1 (Cat# RC200018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LCMT1(Cat# TA503125). Positive lysates LY414046 (100ug) and LC414046 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY414046) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200018 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LCMT1 protein (Cat# TP300018). The protein was produced from HEK293T cells transfected with LCMT1 cDNA clone (Cat# RC200018) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LCMT1
• Synonyms: CGI-68; LCMT; PPMT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200018 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGACGCAGACGACG
  AGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCAGTAAGCATTGGCTACTGGCA
  TGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAGAGGAAAGCCCCTGAAATCAACAGAGGATAT
  TTTGCTCGAGTCCATGGTGTCAGTCAGCTTATAAAGGCATTTCTACGGAAGACAGAATGTCATTGTCAAA
  TTGTCAACCTTGGGGCAGGCATGGATACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAA
  ATATTTTGAGGTTGACTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTA
  TCCAGCCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTGGATTCAAAGA
  GATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAGAAGCTAAAGAAATGTAACAT
  GAATACACAATTGCCAACACTCCTGATAGCTGAATGTGTGCTGGTTTACATGACTCCAGAGCAGTCCGCA
  AACCTCCTGAAGTGGGCAGCCAACAGTTTTGAGAGAGCCATGTTCATAAACTACGAACAGGTGAACATGG
  GTGATCGGTTTGGGCAGATCATGATTGAAAACCTGCGGAGACGCCAGTGTGACCTGGCGGGAGTGGAGAC
  CTGCAAGTCATTAGAGTCACAGAAAGAACGGCTCCTGTCGAATGGGTGGGAAACAGCATCGGCCGTCGAC
  ATGATGGAGTTGTACAACAGGTTACCTCGAGCTGAAGTGAGCAGGATAGAATCACTTGAATTCCTGGATG
  AAATGGAGCTGCTGGAGCAGCTCATGCGGCATTACTGCCTTTGCTGGGCAACCAAAGGAGGAAATGAGCT
  TGGGCTGAAGGAGATAACTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200018 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGY
  FARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPL
  SSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSA
  NLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVD
  MMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016309
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016309.3
• RefSeq Size: 1374 bp
• RefSeq ORF: 1005 bp
• Locus ID: 51451
• UniProt ID: Q9UIC8
• Cytogenetics: 16p12.1
• Domains: LCM
• Protein Pathways: Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
• MW: 38.4 kDa
• Gene Summary: LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008]