LCMT1 (NM_016309) Human Tagged ORF Clone
LCMT1 (Myc-DDK-tagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1
Specifications
Product Data | |
Product Name | LCMT1 (NM_016309) Human Tagged ORF Clone |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LCMT1 |
Synonyms | CGI-68; LCMT; PPMT1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Cell Selection | Neomycin |
Sequence Data |
>RC200018 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGACGCAGACGACG AGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCAGTAAGCATTGGCTACTGGCA TGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAGAGGAAAGCCCCTGAAATCAACAGAGGATAT TTTGCTCGAGTCCATGGTGTCAGTCAGCTTATAAAGGCATTTCTACGGAAGACAGAATGTCATTGTCAAA TTGTCAACCTTGGGGCAGGCATGGATACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAA ATATTTTGAGGTTGACTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTA TCCAGCCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTGGATTCAAAGA GATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAGAAGCTAAAGAAATGTAACAT GAATACACAATTGCCAACACTCCTGATAGCTGAATGTGTGCTGGTTTACATGACTCCAGAGCAGTCCGCA AACCTCCTGAAGTGGGCAGCCAACAGTTTTGAGAGAGCCATGTTCATAAACTACGAACAGGTGAACATGG GTGATCGGTTTGGGCAGATCATGATTGAAAACCTGCGGAGACGCCAGTGTGACCTGGCGGGAGTGGAGAC CTGCAAGTCATTAGAGTCACAGAAAGAACGGCTCCTGTCGAATGGGTGGGAAACAGCATCGGCCGTCGAC ATGATGGAGTTGTACAACAGGTTACCTCGAGCTGAAGTGAGCAGGATAGAATCACTTGAATTCCTGGATG AAATGGAGCTGCTGGAGCAGCTCATGCGGCATTACTGCCTTTGCTGGGCAACCAAAGGAGGAAATGAGCT TGGGCTGAAGGAGATAACTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200018 protein sequence
Red=Cloning site Green=Tags(s) MATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGY FARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPL SSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSA NLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVD MMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016309 |
ORF Size | 1002 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016309.3 |
RefSeq Size | 1374 bp |
RefSeq ORF | 1005 bp |
Locus ID | 51451 |
Cytogenetics | 16p12.1 |
Domains | LCM |
Protein Pathways | Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism |
MW | 38.4 kDa |
Gene Summary | LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
cDNA Clone Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114308 | LCMT1 (untagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1 |
USD 560.00 |
|
RG200018 | LCMT1 (GFP-tagged) - Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1 |
USD 470.00 |
|
RC200018L1 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, Myc-DDK-tagged |
USD 640.00 |
|
RC200018L2 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mGFP tagged |
USD 640.00 |
|
RC200018L3 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, Myc-DDK-tagged |
USD 640.00 |
|
RC200018L4 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mGFP tagged |
USD 640.00 |
USD 350.00
USD 570.00
USD 379.00