LCMT1 (NM_016309) Human Untagged Clone

CAT#: SC114308

LCMT1 (untagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LCMT1
• Synonyms: CGI-68; LCMT; PPMT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114308 sequence for NM_016309 edited (data generated by NextGen Sequencing)
  ATGGCCACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGAC
  GCAGACGACGAGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCA
  GTAAGCATTGGCTACTGGCATGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAG
  AGGAAAGCCCCTGAAATCAACAGAGGATATTTTGCTCGAGTCCATGGTGTCAGTCAGCTT
  ATAAAGGCATTTCTACGGAAGACAGAATGTCATTGTCAAATTGTCAACCTTGGGGCAGGC
  ATGGATACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAAATATTTTGAG
  GTTGACTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTA
  TCCAGCCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTG
  GATTCAAAGAGATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAG
  AAGCTAAAGAAATGTAACATGAATACACAATTGCCAACACTCCTGATAGCTGAATGTGTG
  CTGGTTTACATGACTCCAGAGCAGTCCGCAAACCTCCTGAAGTGGGCAGCCAACAGTTTT
  GAGAGAGCCATGTTCATAAACTACGAACAGGTGAACATGGGTGATCGGTTTGGGCAGATC
  ATGATTGAAAACCTGCGGAGACGCCAGTGTGACCTGGCGGGAGTGGAGACCTGCAAGTCA
  TTAGAGTCACAGAAAGAACGGCTCCTGTCGAATGGGTGGGAAACAGCATCGGCCGTCGAC
  ATGATGGAGTTGTACAACAGGTTACCTCGAGCTGAAGTGAGCAGGATAGAATCACTTGAA
  TTCCTGGATGAAATGGAGCTGCTGGAGCAGCTCATGCGGCATTACTGCCTTTGCTGGGCA
  ACCAAAGGAGGAAATGAGCTTGGGCTGAAGGAGATAACTTATTAA
  
  Clone variation with respect to NM_016309.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016309 unedited
  GGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTGAGCCAG
  GTAGGGCCCTACCCTCTTCTGTTGCTTTCTCCCTGTGGCTCGCGCCGTCCCCCGCCGCCC
  GTCGACCCCGCTTCCATGTCCCTGGCGGACACAGCTCCCAGGAACCTCCACGCCCATGGC
  CACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGACGCAGA
  CGACGAGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCAGTAAG
  CATTGGCTACTGGCATGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAGAGGAA
  AGCCCCTGAAATCAACAGAGGATATTTTGCTCGAGTCCATGGTGTCAGTCAGCTTATAAA
  GGCATTTCTACGGAAGACAGAATGTCATTGTCAAATTGTCAACCTTGGGGCAGGCATGGA
  TACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAAATATTTTGAGGTTGA
  CTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTATCCAG
  CCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTGGATTC
  AAAGAGATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAGAAGCT
  AAAGAAATGTAACATGAATACACAATTGCCAACACTCCTGATAGCTGAATGGTGTGCTGG
  TTTACATGACTCCAGAGCAGTCCGCANACCTNNCTGAGTGGGCAGCCAACANGTTTGAGA
  AGACCATGTTCATAAACTACGAACAGGTGAACAGGGGTGGATCGGNTTGGNGCAGATCAT
  GAATNGAAACCCTGCGAGACGCCAGTGTGACCTGGCGGNNAGTGGAGACCTGCAGTCATT
  AA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016309 unedited
  GACCCCGCCCGAACAACCCGNGCCGCNTCCCCCTTTNNAATTTTTGACTTTGGNCCGCGG
  NCCGCATACNANGATCGNGTTTTTTTTTTTTTTTTTTGGTGGCAAGTGGAAATTATTTAA
  ACAACGACATGTCAACAGGGAACAGGAAGAGGAACATGTGCGACCACTGTAGTGACCAAG
  GCTTCTCAAAAGTGTGGGATGAGACCTGCGGACGAGGCCCGCCCACCGCTCAGGGAGCTT
  GCAGGTCTCCTCCAGGAGGGCAAGTGGCTTCGGCTTCTGGCTCGGCATAAGCCTTCGACA
  GATTAATAAGTTATCTCCTTCAGCCCAAGCTCATTTCCTCCTTTGGTTGCCCAGCAAAGG
  CAGTAATGCCGCATGAGCTGCTCCAGCAGCTCCATTTCATCCAGGAATTCAAGTGATTCT
  ATCCTGCTCACTTCAGCTCGAGGTAACCTGTTGTACAACTCCATCATGTCGACGGCCGAT
  GCTGTTTCCCACCCATTCGACAGGAGCCGTTCTTTCTGTGACTCTAATGACTTGCAGGTC
  TCCACTCCCGCCAGGTCACACTGGCGTCTCCGCAGGTTTTCAATCATGATCTGCCCAAAC
  CGATCACCCATGTTCACCTGTTCGTAGTTTATGAACATGGCTCTCTCAAAACTGTTGGCT
  GCCCACTTCAGGAGGTTTGCGGACTGCTCTGGAGTCATGTAAACCAGCACACATTCAGCT
  ATCAGGAGTGTTGGCAATTGGGTATTCATGTTACATTTCTTTAGCTTCTCTTCCAGTTCA
  GACAGGTCTCGGAGATCTGCTCCAATAACGCCATATCCTCTTTGAATCCAGTATGTGTGC
  ATCCATTTGAAGTGTGTCCTCTGGAAGCAAGTTTTAAAATGGGGCTGGTAAGGGGAGGCT
  TGCCTTTGAACTGGCCAGCTTTTTCGTGAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016309
• Insert Size: 1380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016309.2, NP_057393.2
• RefSeq Size: 1374 bp
• RefSeq ORF: 1005 bp
• Locus ID: 51451
• UniProt ID: Q9UIC8
• Cytogenetics: 16p12.1
• Domains: LCM
• Protein Pathways: Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
• Gene Summary: LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).