Tnfsf11 (NM_011613) Mouse Tagged ORF Clone
CAT#: MR227225
- TrueORF®
Tnfsf11 (Myc-DDK-tagged) - Mouse tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_011613" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tnfsf11 |
Synonyms | Ly109l; ODF; OPGL; RANKL; Trance |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227225 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCGGGCCAGCCGAGACTACGGCAAGTACCTGCGCAGCTCGGAGGAGATGGGCAGCGGCCCCGGCG TCCCACACGAGGGTCCGCTGCACCCCGCGCCTTCTGCACCGGCTCCGGCGCCGCCACCCGCCGCCTCCCG CTCCATGTTCCTGGCCCTCCTGGGGCTGGGACTGGGCCAGGTGGTCTGCAGCATCGCTCTGTTCCTGTAC TTTCGAGCGCAGATGGATCCTAACAGAATATCAGAAGACAGCACTCACTGCTTTTATAGAATCCTGAGAC TCCATGAAAACGCAGGTTTGCAGGACTCGACTCTGGAGAGTGAAGACACACTACCTGACTCCTGCAGGAG GATGAAACAAGCCTTTCAGGGGGCCGTGCAGAAGGAACTGCAACACATTGTGGGGCCACAGCGCTTCTCA GGAGCTCCAGCTATGATGGAAGGCTCATGGTTGGATGTGGCCCAGCGAGGCAAGCCTGAGGCCCAGCCAT TTGCACACCTCACCATCAATGCTGCCAGCATCCCATCGGGTTCCCATAAAGTCACTCTGTCCTCTTGGTA CCACGATCGAGGCTGGGCCAAGATCTCTAACATGACGTTAAGCAACGGAAAACTAAGGGTTAACCAAGAT GGCTTCTATTACCTGTACGCCAACATTTGCTTTCGGCATCATGAAACATCGGGAAGCGTACCTACAGACT ATCTTCAGCTGATGGTGTATGTCGTTAAAACCAGCATCAAAATCCCAAGTTCTCATAACCTGATGAAAGG AGGGAGCACGAAAAACTGGTCGGGCAATTCTGAATTCCACTTTTATTCCATAAATGTTGGGGGATTTTTC AAGCTCCGAGCTGGTGAAGAAATTAGCATTCAGGTGTCCAACCCTTCCCTGCTGGATCCGGATCAAGATG CGACGTACTTTGGGGCTTTCAAAGTTCAGGACATAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227225 protein sequence
Red=Cloning site Green=Tags(s) MRRASRDYGKYLRSSEEMGSGPGVPHEGPLHPAPSAPAPAPPPAASRSMFLALLGLGLGQVVCSIALFLY FRAQMDPNRISEDSTHCFYRILRLHENAGLQDSTLESEDTLPDSCRRMKQAFQGAVQKELQHIVGPQRFS GAPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQD GFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFF KLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011613 |
ORF Size | 951 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011613.2, NP_035743.1 |
RefSeq Size | 2243 bp |
RefSeq ORF | 951 bp |
Locus ID | 21943 |
UniProt ID | O35235 |
Cytogenetics | 14 41.26 cM |
MW | 34.9 kDa |
Gene Summary | Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:24039232). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209550 | Tnfsf11 (untagged) - Mouse tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11), (10ug) |
USD 300.00 |
|
MG227225 | Tnfsf11 (tGFP-tagged) - Mouse tumor necrosis factor (ligand) superfamily member 11 (Tnfsf11), (10ug) |
USD 500.00 |
|
MR227225L3 | Lenti ORF clone of Tnfsf11 (Myc-DDK-tagged) - Mouse tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) |
USD 600.00 |
|
MR227225L4 | Lenti ORF clone of Tnfsf11 (mGFP-tagged) - Mouse tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) |
USD 600.00 |
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