Tnfsf11 (NM_011613) Mouse Untagged Clone

CAT#: MC209550

Tnfsf11 (untagged) - Mouse tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnfsf11
• Synonyms: Ly109l; ODF; OPGL; RANKL; Trance
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209550 representing NM_011613
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCGGGCCAGCCGAGACTACGGCAAGTACCTGCGCAGCTCGGAGGAGATGGGCAGCGGCCCCGGCG
  TCCCACACGAGGGTCCGCTGCACCCCGCGCCTTCTGCACCGGCTCCGGCGCCGCCACCCGCCGCCTCCCG
  CTCCATGTTCCTGGCCCTCCTGGGGCTGGGACTGGGCCAGGTGGTCTGCAGCATCGCTCTGTTCCTGTAC
  TTTCGAGCGCAGATGGATCCTAACAGAATATCAGAAGACAGCACTCACTGCTTTTATAGAATCCTGAGAC
  TCCATGAAAACGCAGGTTTGCAGGACTCGACTCTGGAGAGTGAAGACACACTACCTGACTCCTGCAGGAG
  GATGAAACAAGCCTTTCAGGGGGCCGTGCAGAAGGAACTGCAACACATTGTGGGGCCACAGCGCTTCTCA
  GGAGCTCCAGCTATGATGGAAGGCTCATGGTTGGATGTGGCCCAGCGAGGCAAGCCTGAGGCCCAGCCAT
  TTGCACACCTCACCATCAATGCTGCCAGCATCCCATCGGGTTCCCATAAAGTCACTCTGTCCTCTTGGTA
  CCACGATCGAGGCTGGGCCAAGATCTCTAACATGACGTTAAGCAACGGAAAACTAAGGGTTAACCAAGAT
  GGCTTCTATTACCTGTACGCCAACATTTGCTTTCGGCATCATGAAACATCGGGAAGCGTACCTACAGACT
  ATCTTCAGCTGATGGTGTATGTCGTTAAAACCAGCATCAAAATCCCAAGTTCTCATAACCTGATGAAAGG
  AGGGAGCACGAAAAACTGGTCGGGCAATTCTGAATTCCACTTTTATTCCATAAATGTTGGGGGATTTTTC
  AAGCTCCGAGCTGGTGAAGAAATTAGCATTCAGGTGTCCAACCCTTCCCTGCTGGATCCGGATCAAGATG
  CGACGTACTTTGGGGCTTTCAAAGTTCAGGACATAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011613
• Insert Size: 951 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC131970, AAI31971
• RefSeq Size: 1320 bp
• RefSeq ORF: 951 bp
• Locus ID: 21943
• UniProt ID: O35235
• Cytogenetics: 14 41.26 cM
• Gene Summary: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:24039232). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).[UniProtKB/Swiss-Prot Function]