Pacsin2 (NM_001159510) Mouse Tagged ORF Clone

CAT#: MR222568

Pacsin2 (Myc-DDK-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 3

Product Images

Circular map for MR222568

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pacsin2
• Synonyms: AI197433
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222568 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTCACCTACGATGACTCTGTGGGAGTGGAAGTGTCCAGCGACAGCTTCTGGGAGGTTGGGAACT
  ACAAACGGACTGTGAAGCGGATTGACGATGGCCACCGCCTGTGTGGTGACCTCATGAACTGTCTGCATGA
  GCGGGCACGCATCGAGAAGGCGTATGCACAGCAGCTCACTGAGTGGGCCCGACGCTGGAGGCAGCTGGTA
  GAGAAGGGACCACAGTATGGGACCGTGGAGAAGGCCTGGATAGCTGTCATGTCTGAAGCAGAGAGGGTGA
  GTGAACTGCACCTGGAAGTGAAGGCATCACTGATGAATGAAGACTTTGAGAAGATCAAGAACTGGCAGAA
  GGAAGCCTTTCACAAGCAGATGATGGGAGGCTTCAAGGAGACCAAAGAAGCAGAGGATGGCTTTCGGAAG
  GCCCAGAAGCCCTGGGCCAAGAAGCTGAAAGAGGTGGAAGCGGCAAAGAAGGCGCACCACACAGCGTGCA
  AAGAGGAGAAGCTGGCCATCTCCCGGGAAGCCAACAGCAAGGCAGATCCATCCCTCAACCCTGAGCAGCT
  GAAGAAACTGCAAGACAAGATAGAAAAATGCAAACAGGACGTTCTAAAGACCAAGGACAAGTATGAGAAG
  TCCCTGAAGGAGCTTGATCAGACCACACCCCAGTACATGGAGAACATGGAGCAGGTGTTCGAGCAGTGCC
  AGCAGTTTGAAGAGAAGCGCCTGCGCTTCTTCCGGGAGGTTCTGCTGGAGGTTCAGAAGCACTTGGATCT
  GTCCAATGTGGCTAGCTATAAAACCATTTACCGGGAGCTGGAGCAGAGCATCAAAGCAGCAGATGCGGTA
  GAGGACCTGAGGTGGTTCCGGGCTAACCATGGGCCAGGCATGGCTATGAACTGGCCACAGTTTGAGGAGT
  GGTCTGCAGATCTGAATCGAACTCTCAGCCGGAGAGAGAAGAAGAAGGCTGTTGACGGTGTCACCCTAAC
  AGGGATCAACCAGACAGGTGACCAGTCTGGACAGAACAAGCCTGGCAGCAACCTTAGTGTCCCGAGCAAC
  CCCGCCCAGTCCACGCAGTTACAGTCCAGCTACAACCCCTTCGAGGACGAGGACGACACGGGCAGCAGCA
  TCAGTGAGAAGGAGGACATTAAGGCCAAAAATGTCAGCAGCTATGAGAAGACTCAGACTTACCCCACTGA
  CTGGTCTGATGATGAGTCTAACAACCCTTTCTCCTCCACGGATGCCAACGGGGATTCGAACCCATTTGAT
  GAGGACACGACCTCAGGAACAGAAGTGCGAGTTCGGGCCCTCTATGACTATGAGGGGCAGGAACATGATG
  AGCTGAGCTTCAAGGCTGGGGATGAACTGACCAAGATAGAGGATGAAGATGAACAGGGTTGGTGCAAGGG
  ACGTTTAGACAGCGGCCAGGTTGGCCTATACCCAGCCAACTATGTCGAGGCTATCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222568 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVTYDDSVGVEVSSDSFWEVGNYKRTVKRIDDGHRLCGDLMNCLHERARIEKAYAQQLTEWARRWRQLV
  EKGPQYGTVEKAWIAVMSEAERVSELHLEVKASLMNEDFEKIKNWQKEAFHKQMMGGFKETKEAEDGFRK
  AQKPWAKKLKEVEAAKKAHHTACKEEKLAISREANSKADPSLNPEQLKKLQDKIEKCKQDVLKTKDKYEK
  SLKELDQTTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVASYKTIYRELEQSIKAADAV
  EDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKAVDGVTLTGINQTGDQSGQNKPGSNLSVPSN
  PAQSTQLQSSYNPFEDEDDTGSSISEKEDIKAKNVSSYEKTQTYPTDWSDDESNNPFSSTDANGDSNPFD
  EDTTSGTEVRVRALYDYEGQEHDELSFKAGDELTKIEDEDEQGWCKGRLDSGQVGLYPANYVEAIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159510
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159510.1, NP_001152982.1
• RefSeq Size: 3644 bp
• RefSeq ORF: 1461 bp
• Locus ID: 23970
• UniProt ID: Q9WVE8
• Cytogenetics: 15 E1
• MW: 55.8 kDa
• Gene Summary: Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.[UniProtKB/Swiss-Prot Function]