Pacsin2 (NM_001159510) Mouse Untagged Clone

CAT#: MC216657

Pacsin2 (untagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pacsin2
• Synonyms: AI197433
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216657 representing NM_001159510
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTCACCTACGATGACTCTGTGGGAGTGGAAGTGTCCAGCGACAGCTTCTGGGAGGTTGGGAACT
  ACAAACGGACTGTGAAGCGGATTGACGATGGCCACCGCCTGTGTGGTGACCTCATGAACTGTCTGCATGA
  GCGGGCACGCATCGAGAAGGCGTATGCACAGCAGCTCACTGAGTGGGCCCGACGCTGGAGGCAGCTGGTA
  GAGAAGGGACCACAGTATGGGACCGTGGAGAAGGCCTGGATAGCTGTCATGTCTGAAGCAGAGAGGGTGA
  GTGAACTGCACCTGGAAGTGAAGGCATCACTGATGAATGAAGACTTTGAGAAGATCAAGAACTGGCAGAA
  GGAAGCCTTTCACAAGCAGATGATGGGAGGCTTCAAGGAGACCAAAGAAGCAGAGGATGGCTTTCGGAAG
  GCCCAGAAGCCCTGGGCCAAGAAGCTGAAAGAGGTGGAAGCGGCAAAGAAGGCGCACCACACAGCGTGCA
  AAGAGGAGAAGCTGGCCATCTCCCGGGAAGCCAACAGCAAGGCAGATCCATCCCTCAACCCTGAGCAGCT
  GAAGAAACTGCAAGACAAGATAGAAAAATGCAAACAGGACGTTCTAAAGACCAAGGACAAGTATGAGAAG
  TCCCTGAAGGAGCTTGATCAGACCACACCCCAGTACATGGAGAACATGGAGCAGGTGTTCGAGCAGTGCC
  AGCAGTTTGAAGAGAAGCGCCTGCGCTTCTTCCGGGAGGTTCTGCTGGAGGTTCAGAAGCACTTGGATCT
  GTCCAATGTGGCTAGCTATAAAACCATTTACCGGGAGCTGGAGCAGAGCATCAAAGCAGCAGATGCGGTA
  GAGGACCTGAGGTGGTTCCGGGCTAACCATGGGCCAGGCATGGCTATGAACTGGCCACAGTTTGAGGAGT
  GGTCTGCAGATCTGAATCGAACTCTCAGCCGGAGAGAGAAGAAGAAGGCTGTTGACGGTGTCACCCTAAC
  AGGGATCAACCAGACAGGTGACCAGTCTGGACAGAACAAGCCTGGCAGCAACCTTAGTGTCCCGAGCAAC
  CCCGCCCAGTCCACGCAGTTACAGTCCAGCTACAACCCCTTCGAGGACGAGGACGACACGGGCAGCAGCA
  TCAGTGAGAAGGAGGACATTAAGGCCAAAAATGTCAGCAGCTATGAGAAGACTCAGACTTACCCCACTGA
  CTGGTCTGATGATGAGTCTAACAACCCTTTCTCCTCCACGGATGCCAACGGGGATTCGAACCCATTTGAT
  GAGGACACGACCTCAGGAACAGAAGTGCGAGTTCGGGCCCTCTATGACTATGAGGGGCAGGAACATGATG
  AGCTGAGCTTCAAGGCTGGGGATGAACTGACCAAGATAGAGGATGAAGATGAACAGGGTTGGTGCAAGGG
  ACGTTTAGACAGCGGCCAGGTTGGCCTATACCCAGCCAACTATGTCGAGGCTATCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159510
• Insert Size: 1461 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159510.1, NP_001152982.1
• RefSeq Size: 3644 bp
• RefSeq ORF: 1461 bp
• Locus ID: 23970
• UniProt ID: Q9WVE8
• Cytogenetics: 15 E1
• Gene Summary: Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.