Brs3 (NM_009766) Mouse Tagged ORF Clone

USD 300.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Brs3 (NM_009766) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Brs3
Synonyms BRS-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR221973 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCAAAGGCAGTCTCAGTCACCTAATCAGACTTTAATTTCCATTACAAATGACACAGAAACATCAA
GCTCTGTCGTCTCCAACGATACTACACATAAAGGATGGACCGGAGACAACTCTCCAGGAATAGAAGCACT
GTGTGCCATCTATATCACTTATGCTGGGATCATTTCAGTGGGCATCCTCGGAAATGCTATCCTCATCAAA
GTCTTTTTCAAGACTAAATCCATGCAAACAGTTCCAAATATTTTCATCACCAGCCTGGCTTTCGGAGACC
TGTTACTTCTGCTGACTTGTGTGCCTGTGGATGCAACCCACTACCTGGCAGAAGGATGGCTGTTTGGAAA
GGTCGGTTGTAAAGTGCTTTCCTTCATCCGGCTCACTTCTGTCGGTGTGTCAGTGTTCACGCTAACAATT
CTCAGCGCTGACAGATACAAAGCAGTCGTGAAGCCACTTGAGCGACAGCCCCCCAATGCCATTCTGAAGA
CCTGTGCAAAAGCTGGTGGCATCTGGATCGTGTCTATGATATTTGCTCTGCCAGAGGCTATATTCTCAAA
TGTATACACTTTCCAAGATCCTAACAGAAATGTGACATTTGAATCATGTAACTCCTACCCTATCTCTGAG
AGGCTTTTGCAAGAAATACATTCTCTGTTGTGTTTCTTGGTATTCTACATTATCCCGCTCTCGATTATCT
CTGTCTATTATTCTTTGATTGCCAGGACTCTTTACAAAAGCACCCTGAACATACCGACTGAGGAACAAAG
CCATGCCCGAAAGCAGATTGAATCCCGGAAGAGAATTGCCAAAACAGTTTTGGTGCTGGTGGCTCTGTTC
GCACTCTGCTGGTTGCCGAATCATCTCCTGTATCTCTACCACTCCTTCACTTATGAAAGCTATGCAAACC
ACTCTGATGTCCCTTTTGTTATCATCATTTTCTCTCGGGTGCTGGCTTTCAGTAATTCCTGCGTGAACCC
CTTTGCTCTCTATTGGCTGAGCAAGACCTTCCAGCAGCATTTTAAAGCTCAGCTCTGCTGCCTCAAGGCA
GAGCAGCCTGAGCCTCCTCTTGGTGACATCCCCCTTAACAACCTCACTGTGATGGGGCGGGTCCCAGCTA
CTGGGAGTGCACATGTCTCTGAAATTAGTGTGACCCTGTTCAGTGGCAGTAGTGCCAAGAAGGGAGAGGA
CAAAGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR221973 protein sequence
Red=Cloning site Green=Tags(s)

MSQRQSQSPNQTLISITNDTETSSSVVSNDTTHKGWTGDNSPGIEALCAIYITYAGIISVGILGNAILIK
VFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGKVGCKVLSFIRLTSVGVSVFTLTI
LSADRYKAVVKPLERQPPNAILKTCAKAGGIWIVSMIFALPEAIFSNVYTFQDPNRNVTFESCNSYPISE
RLLQEIHSLLCFLVFYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIAKTVLVLVALF
ALCWLPNHLLYLYHSFTYESYANHSDVPFVIIIFSRVLAFSNSCVNPFALYWLSKTFQQHFKAQLCCLKA
EQPEPPLGDIPLNNLTVMGRVPATGSAHVSEISVTLFSGSSAKKGEDKV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_009766
ORF Size 1200 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009766.1, NM_009766.2, NM_009766.3, NP_033896.2
RefSeq Size 2772 bp
RefSeq ORF 1200 bp
Locus ID 12209
Cytogenetics X A6
MW 44.2 kDa
Gene Summary Role in sperm cell division, maturation, or function. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. [UniProtKB/Swiss-Prot Function]

Citations (1)

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