Pm20d1 (NM_178079) Mouse Tagged ORF Clone

CAT#: MR216980

Pm20d1 (Myc-DDK-tagged) - Mouse peptidase M20 domain containing 1 (Pm20d1)

Product Images

Circular map for MR216980

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pm20d1
• Synonyms: 4732466D17Rik; AI098026
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216980 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGCTACTTGCTAGCTTGCCCGCCTGGGCAGCTGTGCTCCTTCTCTTTTTCGCTACGGTCTCCG
  GATCCACTGGCCCTAGAAGCAGGGAAAATCGGGGGGCGTCCCGGATCCCTTCCCAGTTCAGCGAGGAGGA
  GCGTGTCGCTATAAAAGAGGCGCTGAAAGGTGCCATCCAGATTCCCACAGTGTCTTTCAGCCACGAGGAA
  TCCAACACCACAGCCCTTGCTGAGTTTGGAGAATATATCCGCAAAGCCTTCCCTACAGTGTTCCACAGCA
  GCCTTGTCCAACATGAAGTCGTGGCAAAGTATAGCCACCTGTTCACCATCCAAGGCTCAGACCCCAGTTT
  GCAGCCCTACATGCTGATGGCTCACATTGATGTGGTTCCTGCCCCGGAAGAAGGATGGGAGGTGCCCCCG
  TTCTCAGGCCTGGAACGCAATGGCTTCATCTATGGCCGGGGTGCGCTGGACAACAAAAACTCTGTGATGG
  CGATCCTGCATGCTTTGGAGCTCCTGTTGATCAGAAACTACAGCCCCAAAAGATCTTTCTTCATTGCTTT
  GGGCCATGATGAGGAGGTGTCCGGGGAAAAGGGGGCTCAGAAGATCTCAGCACTCTTACAGGCAAGGGGT
  GTCCAGCTAGCCTTCCTTGTGGATGAAGGGAGCTTTATCTTGGAAGGCTTCATTCCAAACCTCGAGAAGC
  CAGTTGCCATGATTTCAGTCACTGAGAAGGGTGCCCTTGACCTCATGCTGCAAGTAAACATGACTCCAGG
  CCACTCTTCAGCTCCCCCAAAGGAGACAAGCATTGGCATTCTTTCTGCCGCTGTCAGCCGACTGGAGCAG
  ACACCAATGCCGAATATGTTTGGAGGAGGGCCATTGAAGAAGACAATGAAGCTACTGGCAAATGAGTTTT
  CCTTCCCTATCAATATAGTCTTGAGAAACCTGTGGCTATTTCATCCCATTGTGAGCAGGATAATGGAGAG
  GAACCCCATAACAAATGCGCTGGTCCGAACTACCACAGCCCTCACCATGTTCAATGCAGGAATCAAGGTG
  AATGTCATCCCTCCATTGGCTCAGGCTACAATCAACTGCCGAATTCACCCTTCGCAGACAGTACATGAGG
  TCCTAGAACTTGTCAAGAACACCGTGGCTGATGACAGAGTCCAGCTGCATGTGTTGAGATCCTTTGAACC
  CCTGCCCATCAGCCCCTCTGATGACCAGGCCATGGGCTACCAGCTGCTTCAAGAGACCATACGATCTGTC
  TTCCCGGAAGTCGACATCGTCGTCCCCGGTATTTGTATTGCCAATACGGACACCCGACACTATGCCAACA
  TCACCAATGGCATGTACCGGTTCAACCCCCTTCCCCTGAACCCTCAGGACTTCAGTGGTGTCCATGGAAT
  CAATGAGAAAGTTTCCGTTCAGAACTACCAGAACCAGGTGAAGTTCATCTTTGAGTTCATCCAAAATGCC
  GACACTTACAAAGAGCCAGTTCCTCATCTGCATGAACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216980 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAELLASLPAWAAVLLLFFATVSGSTGPRSRENRGASRIPSQFSEEERVAIKEALKGAIQIPTVSFSHEE
  SNTTALAEFGEYIRKAFPTVFHSSLVQHEVVAKYSHLFTIQGSDPSLQPYMLMAHIDVVPAPEEGWEVPP
  FSGLERNGFIYGRGALDNKNSVMAILHALELLLIRNYSPKRSFFIALGHDEEVSGEKGAQKISALLQARG
  VQLAFLVDEGSFILEGFIPNLEKPVAMISVTEKGALDLMLQVNMTPGHSSAPPKETSIGILSAAVSRLEQ
  TPMPNMFGGGPLKKTMKLLANEFSFPINIVLRNLWLFHPIVSRIMERNPITNALVRTTTALTMFNAGIKV
  NVIPPLAQATINCRIHPSQTVHEVLELVKNTVADDRVQLHVLRSFEPLPISPSDDQAMGYQLLQETIRSV
  FPEVDIVVPGICIANTDTRHYANITNGMYRFNPLPLNPQDFSGVHGINEKVSVQNYQNQVKFIFEFIQNA
  DTYKEPVPHLHEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178079
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178079.3, NP_835180.2
• RefSeq Size: 3540 bp
• RefSeq ORF: 1512 bp
• Locus ID: 212933
• UniProt ID: Q8C165
• Cytogenetics: 1 E4
• MW: 55.7 kDa
• Gene Summary: Bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. Thereby, this secreted protein may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.[UniProtKB/Swiss-Prot Function]