Olfr1225 (NM_146891) Mouse Tagged ORF Clone
CAT#: MR215561
- TrueORF®
Olfr1225 (Myc-DDK-tagged) - Mouse olfactory receptor 1225 (Olfr1225)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_146891" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Olfr1225 |
Synonyms | MOR233-11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215561 representing NM_146891
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAACCAGAGCTGTGTCACTGAGTTCATATTCCTGGGACTTTCACAGAACTCAAAAGTTGAGAAAA TACTGTTTTTTATATTTTTGTTGATCTATCTTGCAACTATTGGGGGCAACATGATTATTGTGGTGACCAT CATCTACAGCCCTGCACTGCTGGGCTCCCCAATGTACTTCTTCTTGATATTCCTGTCCTTACTGGATGCT TGCACTTCTTCCACTGTCACCCCCAAGATGATTGTAGACTTCTTCTATGATAGGAAGACCATCTCCTTTG AATGTTGCATGACACAACTGTTTGCTGTGCACTTCTTCACTGGGATGGAGGTGATAGTACTGTCAGCCAT GGCTTATGACCGCTATGTGGCCATTTGCAAGCCTCTTCACTACTCTTCCATCATGAACCGGAGGCTCTGT GGCAATCTTGTGATGGTGTCCTGGGCAGGAGGCTTTTTGCATTCTATCATACAAATTATATTCATGTTGC AGCTGCCCTTCTGTGGACCCAATGTCATTGATCATTACATGTGTGACTTGTTCCCATTACTGAAGCTTGC CTGCACTGACACCTATATATTTGTCATTTTGGTGTTTGCCAACAGTGGGTCTATCTGCATCATAATCTTC TCTATTTTGCTTGTCTCCTATGGTGTCATCTTGTACTCTCTGAGAGCCCACAGCTCTGAAGGGAAATTTA AAGCCCTCTCAACCTGTGGATCCCATATTATTGTTGTGGTTTTGTTTTTTGTCCCATGCATATTAACATA TGCACGACCTATATCTGCATTTTCCTTTGAGAAAAATGCTGTTGTATTTACCACAGTGTTGACCCCATTG CTCAATTCTGTAGTTTATACTTTCAGGAATAAGGAAATGAAGAATGCCATCAGGAAAATGTGGAAGAAAC TGATTGCAGTTTCTGATAAACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215561 representing NM_146891
Red=Cloning site Green=Tags(s) MNNQSCVTEFIFLGLSQNSKVEKILFFIFLLIYLATIGGNMIIVVTIIYSPALLGSPMYFFLIFLSLLDA CTSSTVTPKMIVDFFYDRKTISFECCMTQLFAVHFFTGMEVIVLSAMAYDRYVAICKPLHYSSIMNRRLC GNLVMVSWAGGFLHSIIQIIFMLQLPFCGPNVIDHYMCDLFPLLKLACTDTYIFVILVFANSGSICIIIF SILLVSYGVILYSLRAHSSEGKFKALSTCGSHIIVVVLFFVPCILTYARPISAFSFEKNAVVFTTVLTPL LNSVVYTFRNKEMKNAIRKMWKKLIAVSDKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_146891 |
ORF Size | 933 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146891.2, NP_667102.2 |
RefSeq Size | 1027 bp |
RefSeq ORF | 936 bp |
Locus ID | 258893 |
Cytogenetics | 2 E1 |
MW | 35.1 kDa |
Gene Summary | Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214024 | Olfr1225 (untagged) - Mouse olfactory receptor 1225 (Olfr1225), (10ug) |
USD 330.00 |
|
MG215561 | Olfr1225 (tGFP-tagged) - Mouse olfactory receptor 1225 (Olfr1225), (10ug) |
USD 500.00 |
|
MR215561L3 | Lenti ORF clone of Olfr1225 (Myc-DDK-tagged) - Mouse olfactory receptor 1225 (Olfr1225) |
USD 600.00 |
|
MR215561L4 | Lenti ORF clone of Olfr1225 (mGFP-tagged) - Mouse olfactory receptor 1225 (Olfr1225) |
USD 600.00 |
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