Abcg2 (NM_011920) Mouse Tagged ORF Clone

CAT#: MR209819

Abcg2 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 2 (Abcg2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcg2
• Synonyms: ABC15; ABCP; AI428558; BCRP; Bcrp1; MXR; MXR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209819 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCAGTAATGACCACGTGTTAGTACCAATGTCGCAGAGAAACAACAACGGCCTTCCTAGGACGA
  ACTCCAGAGCCGTTAGGACGCTCGCAGAAGGAGATGTGTTGAGTTTTCATCACATCACCTATCGAGTGAA
  AGTAAAGAGTGGGTTTCTAGTCCGGAAAACAGTTGAGAAAGAAATACTATCAGATATCAATGGGATCATG
  AAACCTGGCCTTAATGCTATTCTGGGACCCACAGGCGGAGGCAAGTCTTCGTTGCTAGATGTCTTAGCAG
  CAAGGAAAGATCCAAAGGGATTATCTGGAGATGTTTTGATAAATGGAGCACCTCAACCTGCCCACTTCAA
  ATGCTGTTCAGGTTATGTGGTTCAAGATGACGTTGTGATGGGCACCCTGACAGTGAGAGAAAACTTACAG
  TTCTCAGCAGCTCTTCGACTTCCAACAACTATGAAGAATCATGAAAAAAATGAACGGATTAACACAATCA
  TTAAAGAGTTAGGTCTGGAAAAAGTAGCAGATTCTAAGGTCGGAACTCAGTTTATCCGTGGCATCTCTGG
  AGGAGAAAGAAAAAGGACAAGCATAGGGATGGAGCTGATCACTGACCCTTCCATCCTCTTCCTGGATGAG
  CCCACGACTGGTTTGGACTCAAGCACAGCGAATGCTGTCCTTTTGCTCCTGAAAAGGATGTCTAAACAGG
  GTCGAACAATCATCTTCTCCATTCATCAGCCTCGGTATTCCATCTTTAAGCTGTTTGACAGCCTCACCTT
  ACTGGCTTCCGGGAAACTCGTGTTCCATGGGCCAGCACAGAAGGCCTTGGAGTACTTTGCATCAGCAGGT
  TACCACTGTGAGCCCTACAACAACCCTGCGGATTTTTTCCTTGATGTCATCAATGGAGATTCTTCTGCTG
  TGATGTTAAATAGAGAGGAACAAGACAATGAAGCAAACAAGACTGAAGAGCCTTCCAAGGGAGAGAAGCC
  AGTAATAGAAAATTTATCTGAGTTTTATATCAACTCTGCCATCTATGGAGAAACAAAAGCTGAATTAGAT
  CAACTTCCAGGAGCTCAGGAAAAGAAAGGAACATCGGCCTTCAAAGAGCCAGTCTATGTTACCTCTTTCT
  GTCACCAGCTCCGATGGATTGCCAGGCGCTCATTTAAAAACTTGCTCGGGAACCCTCAAGCTTCTGTTGC
  TCAGTTAATTGTTACAGTCATACTGGGGCTTATTATTGGTGCCATTTACTTTGATCTGAAATATGATGCC
  GCTGGAATGCAAAATAGAGCTGGAGTTTTGTTTTTCCTGACTACCAACCAGTGTTTTTCCAGTGTGTCAG
  CTGTGGAGCTGTTCGTAGTGGAGAAGAAACTCTTCATACATGAGTACATCAGTGGATATTACAGAGTGTC
  TTCTTACTTCTTTGGAAAGGTGATGTCTGATTTACTCCCCATGAGGTTCTTGCCAAGTGTTATATTCACT
  TGTGTATTATACTTCATGTTAGGACTGAAGAAGACGGTGGATGCTTTTTTCATCATGATGTTTACCCTTA
  TAATGGTGGCTTATACGGCCAGTTCCATGGCACTGGCCATAGCCACAGGCCAAAGTGTGGTGTCTGTAGC
  AACACTTCTCATGACAATCGCTTTTGTATTTATGATGCTCTTTTCTGGCCTCTTGGTGAATCTCAGAACC
  ATTGGGCCTTGGCTGTCCTGGCTTCAGTACTTTAGCATTCCTCGATATGGCTTCACAGCTTTGCAGTATA
  ATGAATTCTTGGGACAAGAGTTTTGTCCAGGATTCAATGTAACGGACAACAGCACTTGTGTTAACAGCTA
  TGCAATATGTACTGGTAACGAGTACTTGATAAATCAGGGCATCGAACTGTCACCTTGGGGACTGTGGAAG
  AATCATGTGGCCCTGGCTTGTATGATTATTATCTTCCTCACAATTGCCTACCTGAAATTGTTGTTTCTTA
  AAAAGTATTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209819 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSSNDHVLVPMSQRNNNGLPRTNSRAVRTLAEGDVLSFHHITYRVKVKSGFLVRKTVEKEILSDINGIM
  KPGLNAILGPTGGGKSSLLDVLAARKDPKGLSGDVLINGAPQPAHFKCCSGYVVQDDVVMGTLTVRENLQ
  FSAALRLPTTMKNHEKNERINTIIKELGLEKVADSKVGTQFIRGISGGERKRTSIGMELITDPSILFLDE
  PTTGLDSSTANAVLLLLKRMSKQGRTIIFSIHQPRYSIFKLFDSLTLLASGKLVFHGPAQKALEYFASAG
  YHCEPYNNPADFFLDVINGDSSAVMLNREEQDNEANKTEEPSKGEKPVIENLSEFYINSAIYGETKAELD
  QLPGAQEKKGTSAFKEPVYVTSFCHQLRWIARRSFKNLLGNPQASVAQLIVTVILGLIIGAIYFDLKYDA
  AGMQNRAGVLFFLTTNQCFSSVSAVELFVVEKKLFIHEYISGYYRVSSYFFGKVMSDLLPMRFLPSVIFT
  CVLYFMLGLKKTVDAFFIMMFTLIMVAYTASSMALAIATGQSVVSVATLLMTIAFVFMMLFSGLLVNLRT
  IGPWLSWLQYFSIPRYGFTALQYNEFLGQEFCPGFNVTDNSTCVNSYAICTGNEYLINQGIELSPWGLWK
  NHVALACMIIIFLTIAYLKLLFLKKYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011920
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011920.1, NM_011920.2, NM_011920.3, NP_036050.1
• RefSeq Size: 2493 bp
• RefSeq ORF: 1974 bp
• Locus ID: 26357
• UniProt ID: Q7TMS5
• Cytogenetics: 6 27.82 cM
• MW: 73 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, the human protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. This protein likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. [provided by RefSeq, Jul 2008]