Kars (BC027356) Mouse Tagged ORF Clone

SKU MR209528

Kars (Myc-DDK-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Kars
• Synonyms: AA589550; AL024334; AL033315; AL033367; D8Ertd698e; D8Wsu108e; LysRS; mKIAA0070
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209528 representing BC027356; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTGATGCAAGCTGCTGTCAGGCTTGTTAGGGGGGCCCTGCGCCAAACCTCATGGGCAGAATGGGGTC; AGAGGGAACTTCGACTGGGCCACCTTGCTCCTTTCACAACGCTCCACAAGGACCAGCCACTTTCTGATAG; AAGAAGTGAGCTGAAGAGGCGTCTGAAAGCTGAGAAGAAACTGGCAGAGAAGGAGGCCAAGCAGAAAGAG; CTGAGTGAGAAACAGCTAAACCAGACTGCTTCCGCTCCCAACCACACGGCTGACAATGGCGTGGGTGCTG; AGGAGGAGACTCTGGACCCAAATCAATACTACAAGATCCGAAGTCAAGCCGTCCAGCAGCTGAAGGTCAC; TGGGGAGGATCCGTACCCACACAAGTTCCACGTGGACATCTCACTCACTCAGTTCATCCAAGAATATAGT; CACCTGCAGCCTGGGGACCACCTGACTGATGTCACCCTCAAAGTGGCAGGCCGCATCCACGCCAAGAGGG; CCTCTGGAGGGAAGCTCATCTTCTATGACCTGCGAGGAGAGGGGGTCAAGTTACAAGTCATGGCCAACTC; CAGGAATTACAAATCAGAGGAGGAATTTGTTCATATCAATAACAAACTGCGCCGGGGAGACATAATTGGA; GTTGAGGGCAATCCCGGGAAAACCAAGAAGGGCGAGCTGAGCATCATCCCCCAGGAGATCACGCTGCTGT; CCCCCTGCTTGCACATGCTGCCTCATCTTCACTTTGGCCTCAAAGACAAGGAAACACGGTATCGTCAAAG; ATACTTGGACTTGATCCTGAACGACTTTGTGAGGCAGAAATTTATCGTCCGCTCTAAGATCATCACATAC; ATAAGAAGTTTCTTGGATGAGCTGGGCTTCCTAGAGATCGAAACTCCCATGATGAACATCATTCCCGGGG; GAGCTGTGGCCAAGCCTTTCATCACCTATCACAATGAGCTGGACATGAACTTGTATATGAGAATTGCTCC; AGAACTCTACCACAAGATGCTGGTGGTTGGTGGCATTGACCGGGTTTATGAAATTGGGCGCCAGTTCCGG; AACGAAGGGATTGATTTGACTCACAATCCTGAGTTCACCACCTGTGAGTTCTACATGGCCTATGCAGACT; ATCATGACCTCATGGAGATCACAGAGAAGATGCTGTCAGGGATGGTGAAGAGCATTACAGGCAGTTACAA; GATCACCTACCACCCAGATGGGCCGGAAGGCCAAGCCTACGAGGTCGACTTCACCCCACCCTTCCGAAGA; ATCAGCATGGTAGAAGAGCTTGAGAAAGCCCTAGGTGTGAAGCTGCCAGAAACCAGTCTCTTTGAAACTG; AAGAAACTCGGAAAATTCTTGATGATATTTGTGTTGCAAAAGCTGTTGAATGCCCCCCACCTCGGACCAC; AGCCAGGCTCCTTGATAAGCTCGTTGGCGAGTTCCTCGAAGTCACATGCATCAGCCCTACCTTCATCTGT; GATCACCCACAGATCATGAGTCCTTTGGCCAAATGGCACCGCTCCAAAGAGGGTCTCACGGAGCGCTTTG; AGCTGTTTGTCATGAAGAAGGAGATATGCAATGCCTATACTGAGCTGAATGACCCCGTGCGGCAGAGGCA; GCTGTTTGAGGAGCAGGCCAAGGCCAAGGCTGCCGGTGATGACGAGGCCATGTTCATAGACGAGAACTTC; TGTACTGCCCTGGAATACGGGCTGCCTCCCACAGCGGGCTGGGGCATGGGCATTGATCGGCTCACCATGT; TTCTCACCGATTCCAACAATATCAAGGAAGTACTTCTGTTTCCTGCCATGAAGCCAGAGGACAAGAAGGA; AACTGCAGCGACCACTGAAACCCCAGAGAGCACAGAGGCCAGCCCCTCTGTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209528 representing BC027356; Red=Cloning site Green=Tags(s); ; MLMQAAVRLVRGALRQTSWAEWGQRELRLGHLAPFTTLHKDQPLSDRRSELKRRLKAEKKLAEKEAKQKE; LSEKQLNQTASAPNHTADNGVGAEEETLDPNQYYKIRSQAVQQLKVTGEDPYPHKFHVDISLTQFIQEYS; HLQPGDHLTDVTLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFVHINNKLRRGDIIG; VEGNPGKTKKGELSIIPQEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIVRSKIITY; IRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFR; NEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVKSITGSYKITYHPDGPEGQAYEVDFTPPFRR; ISMVEELEKALGVKLPETSLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCISPTFIC; DHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENF; CTALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPEDKKETAATTETPESTEASPSV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027356
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC027356.1
• RefSeq Size: 2205 bp
• RefSeq ORF: 1874 bp
• Locus ID: 85305
• Cytogenetics: 8 58.27 cM
• MW: 80.9 kDa
• Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.[UniProtKB/Swiss-Prot Function]