Chrnb2 (NM_009602) Mouse Tagged ORF Clone

CAT#: MR208043

Chrnb2 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal) (Chrnb2)

Product Images

Circular map for MR208043

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrnb2
• Synonyms: Acrb-2; Acrb2; C030030P04Rik; [b]2-nAchR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208043 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGTGCTCCAACTCTATGGCGCTGCTGTTCAGCTTTGGCCTCCTTTGGCTGTGTTCAGGGGTTT
  TGGGTACTGACACAGAGGAGCGGCTGGTGGAGCATCTCTTGGATCCTTCCCGCTATAACAAGCTGATCCG
  TCCAGCTACTAATGGCTCTGAGCTGGTGACTGTACAGCTCATGGTATCATTGGCACAGCTCATCAGTGTG
  CACGAGCGGGAGCAGATCATGACCACCAACGTCTGGCTGACCCAGGAGTGGGAAGATTATCGCCTCACAT
  GGAAGCCTGAGGATTTCGACAATATGAAGAAAGTCCGACTCCCTTCTAAGCACATCTGGCTCCCAGATGT
  GGTTCTATACAACAATGCTGACGGCATGTACGAAGTCTCCTTCTATTCCAATGCTGTGGTCTCCTATGAT
  GGCAGCATCTTTTGGCTACCGCCTGCCATCTACAAGAGCGCATGCAAGATTGAGGTGAAGCACTTCCCAT
  TTGACCAGCAGAACTGCACCATGAAGTTCCGCTCCTGGACCTACGACCGCACTGAGATTGACCTGGTGCT
  CAAAAGCGATGTGGCCAGCCTGGACGACTTCACACCTAGTGGGGAGTGGGACATCATCGCACTGCCAGGC
  CGACGCAACGAGAACCCAGACGACTCCACCTACGTGGACATCACCTACGACTTCATCATTCGTCGCAAAC
  CGCTCTTCTACACCATCAACCTCATCATCCCCTGCGTACTCATCACCTCGCTGGCCATCCTGGTCTTCTA
  CCTGCCCTCAGACTGTGGTGAAAAGATGACACTTTGTATTTCTGTGCTGCTGGCGCTCACGGTGTTCCTG
  CTGCTCATCTCCAAGATTGTGCCTCCCACCTCCCTCGACGTACCGCTGGTGGGAAAGTACCTCATGTTCA
  CCATGGTGCTAGTCACCTTCTCCATCGTCACTAGCGTGTGTGTGCTCAATGTGCACCACCGTTCGCCTAC
  CACGCACACCATGGCGCCCTGGGTCAAGGTGGTCTTCCTGGAGAAGCTGCCCACCCTCCTCTTCCTGCAG
  CAGCCACGCCACCGCTGTGCACGTCAACGCCTGCGCTTGCGAAGGCGCCAGCGGGAACGTGAGGGGGCAG
  GCACACTATTCTTCCGCGAAGGTCCTGCAGCTGACCCATGTACCTGCTTTGTCAATCCTGCATCAATGCA
  GGGCTTGGCTGGGGCTTTCCAGGCTGAGCCCGCTGCAGCCGGTCTGGGGCGCTCGATGGGCCCATGCAGC
  TGTGGCCTCCGGGAAGCTGTGGACGGTGTACGCTTCATTGCGGACCATATGCGAAGTGAAGATGATGACC
  AGAGTGTGAGGGAGGATTGGAAATACGTTGCCATGGTGATCGACCGCCTGTTCCTCTGGATCTTTGTCTT
  TGTCTGTGTCTTTGGGACCATTGGCATGTTCCTGCAGCCTCTCTTCCAGAACTACACTGCCACGACCTTC
  CTTCACTCTGACCACTCAGCTCCCAGCTCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208043 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARCSNSMALLFSFGLLWLCSGVLGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISV
  HEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYD
  GSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSDVASLDDFTPSGEWDIIALPG
  RRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFL
  LLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPTLLFLQ
  QPRHRCARQRLRLRRRQREREGAGTLFFREGPAADPCTCFVNPASMQGLAGAFQAEPAAAGLGRSMGPCS
  CGLREAVDGVRFIADHMRSEDDDQSVREDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLFQNYTATTF
  LHSDHSAPSSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009602
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009602.4
• RefSeq Size: 5405 bp
• RefSeq ORF: 1506 bp
• Locus ID: 11444
• UniProt ID: Q9ERK7
• Cytogenetics: 3 39.19 cM
• MW: 57.1 kDa
• Gene Summary: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions.[UniProtKB/Swiss-Prot Function]