Chrnb2 Rabbit Polyclonal Antibody

CAT#: TA389023

Anti-Nicotinic Acetylcholine Receptor (nAChR) β2 Antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000
  WB Brain: 1:1000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein from the cytoplasmic loop of the β2 subunit of mouse nAChR.
• Specificity: Specific for endogenous levels of the ~52 kDa nAChRβ2 protein.
• Formulation: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Concentration: lot specific
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 52
• Gene Name: cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
• Database Link:
  Entrez Gene 11444 Mouse
  UniProt ID Q9ERK7
• Background: Nicotinic acetylcholine receptors (nAChRs) are ionotropic, cholinergic receptors that are divided into 2 types; muscle type and neuronal type. Neuronal nAChRs are pentameric ion channels consisting of 5 identical (homopentamers) or different (heteropentamers) subunits. Heteropentameric neuronal nAChRs mediate fast synaptic transmission in the autonomic nervous system. The predominant hetero-oligomeric nAChR in the CNS contain the subunits α4β2, whereas α3β4 prevail in the PNS. However, the expression of these subunits varies not only by region but also during development (Scholze et al 2011). In the brain, β2-containing receptors greatly outnumber receptors that contain β4 (McGehee & Role, 1995; Albuquerque, et al., 2009), and in most brain regions, targeted deletion of the β2 subunit virtually abolishes [3H]-epibatidine binding and receptor autoradiography (Zoli, et al., 1998) due to the absence of a β subunit required to form functional nAChRs (Champtiaux & Changeux, 2004).
• Synonyms: EFNL3; nAChRB2
• Note: Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein antigen was coupled.