Acads (NM_007383) Mouse Tagged ORF Clone
CAT#: MR206476
- TrueORF®
Acads (Myc-DDK-tagged) - Mouse acyl-Coenzyme A dehydrogenase, short chain (Acads), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_007383" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Acads |
Synonyms | AI196007; Bcd-1; Bcd1; Hdlq; Hdlq8; SC; SCAD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206476 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGCCTTGCTCGCCCGGGCCCGTGGCCCTCTCCGTAGAGCTCTCGGTGTTCGGGACTGGCGAC GGTTACACACTGTTTACCAGTCTGTGGAGCTGCCTGAGACACACCAGATGTTGCGTCAGACATGCCGTGA CTTTGCCGAGAAGGAGTTGGTCCCCATTGCGGCCCAGCTGGACAGGGAGCATCTCTTCCCCACAGCTCAG GTTAAGAAGATGGGTGAGCTCGGGCTGCTGGCCATGGATGTGCCAGAGGAGCTGAGTGGTGCAGGCTTGG ATTACCTGGCCTACTCCATCGCCCTGGAGGAGATCAGCCGTGCCTGCGCCTCCACGGGAGTTATCATGAG CGTCAACAATTCTCTCTACTTGGGACCCATTCTGAAGTTTGGATCCGCACAGCAGAAGCAACAGTGGATC ACCCCTTTCACCAATGGTGACAAAATCGGCTGTTTTGCCCTCAGTGAGCCAGGCAATGGCAGTGATGCTG GAGCCGCTTCCACCACTGCCCGGGAAGAGGGTGACTCATGGGTCCTCAACGGCACCAAAGCTTGGATCAC CAACTCCTGGGAGGCTTCCGCCACGGTGGTATTTGCCAGCACAGACAGGTCCCGGCAGAACAAGGGTATC AGTGCCTTCCTGGTTCCCATGCCAACTCCTGGGCTCACGCTGGGCAAGAAGGAAGACAAGCTGGGCATCC GGGCCTCCTCCACAGCTAACCTCATCTTTGAGGACTGCCGGATCCCCAAGGAGAACCTGCTTGGGGAGCC TGGGATGGGCTTCAAAATAGCCATGCAAACCCTGGACATGGGTCGCATTGGCATCGCCTCCCAGGCCCTG GGCATCGCCCAGGCCTCCCTGGATTGTGCTGTGAAGTATGCCGAGAACCGCAATGCCTTTGGGGCACCGC TCACCAAGCTCCAAAATATCCAGTTCAAGCTGGCAGACATGGCCCTGGCCCTGGAGAGTGCCCGCCTGCT GACCTGGCGTGCTGCCATGTTGAAAGACAACAAGAAACCTTTCACCAAGGAGTCCGCCATGGCCAAACTG GCTGCATCGGAGGCTGCAACCGCCATTAGCCACCAGGCCATCCAGATCCTGGGCGGCATGGGGTATGTGA CAGAGATGCCGGCTGAGCGGTACTACCGAGATGCCCGCATCACTGAGATCTACGAAGGGACCAGCGAAAT CCAGAGACTGGTGATCGCTGGGCATCTGCTCCGGAGCTACCGGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206476 protein sequence
Red=Cloning site Green=Tags(s) MAAALLARARGPLRRALGVRDWRRLHTVYQSVELPETHQMLRQTCRDFAEKELVPIAAQLDREHLFPTAQ VKKMGELGLLAMDVPEELSGAGLDYLAYSIALEEISRACASTGVIMSVNNSLYLGPILKFGSAQQKQQWI TPFTNGDKIGCFALSEPGNGSDAGAASTTAREEGDSWVLNGTKAWITNSWEASATVVFASTDRSRQNKGI SAFLVPMPTPGLTLGKKEDKLGIRASSTANLIFEDCRIPKENLLGEPGMGFKIAMQTLDMGRIGIASQAL GIAQASLDCAVKYAENRNAFGAPLTKLQNIQFKLADMALALESARLLTWRAAMLKDNKKPFTKESAMAKL AASEAATAISHQAIQILGGMGYVTEMPAERYYRDARITEIYEGTSEIQRLVIAGHLLRSYRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007383 |
ORF Size | 1239 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007383.3 |
RefSeq Size | 1881 bp |
RefSeq ORF | 1239 bp |
Locus ID | 11409 |
UniProt ID | Q07417 |
Cytogenetics | 5 F |
MW | 44.9 kDa |
Gene Summary | This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the Acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum at C(four)-CoA. In mice, deficiency of this gene has been linked to cold sensitivity and increased high-density lipoprotein levels. [provided by RefSeq, Nov 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201297 | Acads (untagged) - Mouse acyl-Coenzyme A dehydrogenase, short chain (Acads), nuclear gene encoding mitochondrial protein, (10ug) |
USD 457.00 |
|
MG206476 | Acads (tGFP-tagged) - Mouse acyl-Coenzyme A dehydrogenase, short chain (Acads) |
USD 657.00 |
|
MR206476L3 | Lenti ORF clone of Acads (Myc-DDK-tagged) - Mouse acyl-Coenzyme A dehydrogenase, short chain (Acads), nuclear gene encoding mitochondrial protein |
USD 757.00 |
|
MR206476L4 | Lenti ORF clone of Acads (mGFP-tagged) - Mouse acyl-Coenzyme A dehydrogenase, short chain (Acads), nuclear gene encoding mitochondrial protein |
USD 757.00 |
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