Acads (NM_007383) Mouse Tagged ORF Clone

CAT#: MG206476

Acads (tGFP-tagged) - Mouse acyl-Coenzyme A dehydrogenase, short chain (Acads)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acads
• Synonyms: AI196007; Bcd-1; Bcd1; Hdlq; Hdlq8; SC; SCAD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206476 representing NM_007383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCGCCTTGCTCGCCCGGGCCCGTGGCCCTCTCCGTAGAGCTCTCGGTGTTCGGGACTGGCGAC
  GGTTACACACTGTTTACCAGTCTGTGGAGCTGCCTGAGACACACCAGATGTTGCGTCAGACATGCCGTGA
  CTTTGCCGAGAAGGAGTTGGTCCCCATTGCGGCCCAGCTGGACAGGGAGCATCTCTTCCCCACAGCTCAG
  GTTAAGAAGATGGGTGAGCTCGGGCTGCTGGCCATGGATGTGCCAGAGGAGCTGAGTGGTGCAGGCTTGG
  ATTACCTGGCCTACTCCATCGCCCTGGAGGAGATCAGCCGTGCCTGCGCCTCCACGGGAGTTATCATGAG
  CGTCAACAATTCTCTCTACTTGGGACCCATTCTGAAGTTTGGATCCGCACAGCAGAAGCAACAGTGGATC
  ACCCCTTTCACCAATGGTGACAAAATCGGCTGTTTTGCCCTCAGTGAGCCAGGCAATGGCAGTGATGCTG
  GAGCCGCTTCCACCACTGCCCGGGAAGAGGGTGACTCATGGGTCCTCAACGGCACCAAAGCTTGGATCAC
  CAACTCCTGGGAGGCTTCCGCCACGGTGGTATTTGCCAGCACAGACAGGTCCCGGCAGAACAAGGGTATC
  AGTGCCTTCCTGGTTCCCATGCCAACTCCTGGGCTCACGCTGGGCAAGAAGGAAGACAAGCTGGGCATCC
  GGGCCTCCTCCACAGCTAACCTCATCTTTGAGGACTGCCGGATCCCCAAGGAGAACCTGCTTGGGGAGCC
  TGGGATGGGCTTCAAAATAGCCATGCAAACCCTGGACATGGGTCGCATTGGCATCGCCTCCCAGGCCCTG
  GGCATCGCCCAGGCCTCCCTGGATTGTGCTGTGAAGTATGCCGAGAACCGCAATGCCTTTGGGGCACCGC
  TCACCAAGCTCCAAAATATCCAGTTCAAGCTGGCAGACATGGCCCTGGCCCTGGAGAGTGCCCGCCTGCT
  GACCTGGCGTGCTGCCATGTTGAAAGACAACAAGAAACCTTTCACCAAGGAGTCCGCCATGGCCAAACTG
  GCTGCATCGGAGGCTGCAACCGCCATTAGCCACCAGGCCATCCAGATCCTGGGCGGCATGGGGTATGTGA
  CAGAGATGCCGGCTGAGCGGTACTACCGAGATGCCCGCATCACTGAGATCTACGAAGGGACCAGCGAAAT
  CCAGAGACTGGTGATCGCTGGGCATCTGCTCCGGAGCTACCGGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206476 representing NM_007383
  Red=Cloning site Green=Tags(s)
  
  MAAALLARARGPLRRALGVRDWRRLHTVYQSVELPETHQMLRQTCRDFAEKELVPIAAQLDREHLFPTAQ
  VKKMGELGLLAMDVPEELSGAGLDYLAYSIALEEISRACASTGVIMSVNNSLYLGPILKFGSAQQKQQWI
  TPFTNGDKIGCFALSEPGNGSDAGAASTTAREEGDSWVLNGTKAWITNSWEASATVVFASTDRSRQNKGI
  SAFLVPMPTPGLTLGKKEDKLGIRASSTANLIFEDCRIPKENLLGEPGMGFKIAMQTLDMGRIGIASQAL
  GIAQASLDCAVKYAENRNAFGAPLTKLQNIQFKLADMALALESARLLTWRAAMLKDNKKPFTKESAMAKL
  AASEAATAISHQAIQILGGMGYVTEMPAERYYRDARITEIYEGTSEIQRLVIAGHLLRSYRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007383
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007383.3
• RefSeq Size: 1880 bp
• RefSeq ORF: 1239 bp
• Locus ID: 11409
• UniProt ID: Q07417
• Cytogenetics: 5 F
• Gene Summary: This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the Acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum at C(four)-CoA. In mice, deficiency of this gene has been linked to cold sensitivity and increased high-density lipoprotein levels. [provided by RefSeq, Nov 2012]