Arg1 (NM_007482) Mouse Tagged ORF Clone

CAT#: MR204674

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  • TrueORF®

Arg1 (Myc-DDK-tagged) - Mouse arginase, liver (Arg1)



  "NM_007482" in other vectors (4)

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Reconstitution Protocol

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USD 305.00


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Size
    • 10 ug


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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Arg1
Synonyms AI; AI256583; Arg-1; PGIF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204674 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCCAAGCCAAAGTCCTTAGAGATTATCGGAGCGCCTTTCTCAAAAGGACAGCCTCGAGGAGGGG
TAGAGAAAGGCCCTGCAGCACTGAGGAAAGCTGGTCTGCTGGAAAAACTTAAAGAAACAGAGTATGACGT
GAGAGACCACGGGGACCTGGCCTTTGTTGATGTCCCTAATGACAGCTCCTTTCAAATTGTGAAGAACCCA
CGGTCTGTGGGGAAAGCCAATGAAGAGCTGGCTGGTGTGGTGGCAGAGGTCCAGAAGAATGGAAGAGTCA
GTGTGGTGCTGGGTGGAGACCACAGTCTGGCAGTTGGAAGCATCTCTGGCCACGCCAGGGTCCACCCTGA
CCTATGTGTCATTTGGGTGGATGCTCACACTGACATCAACACTCCCCTGACAACCAGCTCTGGGAATCTG
CATGGGCAACCTGTGTCCTTTCTCCTGAAGGAACTGAAAGGAAAGTTCCCAGATGTACCAGGATTCTCCT
GGGTGACTCCCTGCATATCTGCCAAAGACATCGTGTACATTGGCTTGCGAGACGTAGACCCTGGGGAACA
CTATATAATAAAAACTCTGGGAATTAAGTATTTCTCCATGACTGAAGTAGACAAGCTGGGGATTGGCAAG
GTGATGGAAGAGACCTTCAGCTACCTGCTGGGAAGGAAGAAAAGGCCGATTCACCTGAGCTTTGATGTCG
ATGGGCTGGACCCAGCATTCACCCCGGCGACCGGCACCCCGGTTCTGGGAGGCCTATCTTACAGAGAAGG
TCTCTACATCACAGAAGAAATTTACAAGACAGGGCTCCTTTCAGGACTAGATATCATGGAAGTGAACCCA
ACTCTTGGGAAGACAGCAGAGGAGGTGAAGAGTACTGTGAACACGGCAGTGGCTTTAACCTTGGCTTGTT
TCGGAACTCAACGGGAGGGTAACCATAAGCCAGGGACTGACTACCTTAAACCACCTAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204674 protein sequence
Red=Cloning site Green=Tags(s)

MSSKPKSLEIIGAPFSKGQPRGGVEKGPAALRKAGLLEKLKETEYDVRDHGDLAFVDVPNDSSFQIVKNP
RSVGKANEELAGVVAEVQKNGRVSVVLGGDHSLAVGSISGHARVHPDLCVIWVDAHTDINTPLTTSSGNL
HGQPVSFLLKELKGKFPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYIIKTLGIKYFSMTEVDKLGIGK
VMEETFSYLLGRKKRPIHLSFDVDGLDPAFTPATGTPVLGGLSYREGLYITEEIYKTGLLSGLDIMEVNP
TLGKTAEEVKSTVNTAVALTLACFGTQREGNHKPGTDYLKPPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007482
ORF Size 972 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007482.2, NP_031508.1
RefSeq Size 1489 bp
RefSeq ORF 972 bp
Locus ID 11846
UniProt ID Q61176
Cytogenetics 10 A4
MW 34.8 kDa
Gene Summary Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.[UniProtKB/Swiss-Prot Function]

Other Versions

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