Arg1 (NM_007482) Mouse Tagged ORF Clone

CAT#: MR204674

Arg1 (Myc-DDK-tagged) - Mouse arginase, liver (Arg1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arg1
• Synonyms: AI; AI256583; Arg-1; PGIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204674 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCAAGCCAAAGTCCTTAGAGATTATCGGAGCGCCTTTCTCAAAAGGACAGCCTCGAGGAGGGG
  TAGAGAAAGGCCCTGCAGCACTGAGGAAAGCTGGTCTGCTGGAAAAACTTAAAGAAACAGAGTATGACGT
  GAGAGACCACGGGGACCTGGCCTTTGTTGATGTCCCTAATGACAGCTCCTTTCAAATTGTGAAGAACCCA
  CGGTCTGTGGGGAAAGCCAATGAAGAGCTGGCTGGTGTGGTGGCAGAGGTCCAGAAGAATGGAAGAGTCA
  GTGTGGTGCTGGGTGGAGACCACAGTCTGGCAGTTGGAAGCATCTCTGGCCACGCCAGGGTCCACCCTGA
  CCTATGTGTCATTTGGGTGGATGCTCACACTGACATCAACACTCCCCTGACAACCAGCTCTGGGAATCTG
  CATGGGCAACCTGTGTCCTTTCTCCTGAAGGAACTGAAAGGAAAGTTCCCAGATGTACCAGGATTCTCCT
  GGGTGACTCCCTGCATATCTGCCAAAGACATCGTGTACATTGGCTTGCGAGACGTAGACCCTGGGGAACA
  CTATATAATAAAAACTCTGGGAATTAAGTATTTCTCCATGACTGAAGTAGACAAGCTGGGGATTGGCAAG
  GTGATGGAAGAGACCTTCAGCTACCTGCTGGGAAGGAAGAAAAGGCCGATTCACCTGAGCTTTGATGTCG
  ATGGGCTGGACCCAGCATTCACCCCGGCGACCGGCACCCCGGTTCTGGGAGGCCTATCTTACAGAGAAGG
  TCTCTACATCACAGAAGAAATTTACAAGACAGGGCTCCTTTCAGGACTAGATATCATGGAAGTGAACCCA
  ACTCTTGGGAAGACAGCAGAGGAGGTGAAGAGTACTGTGAACACGGCAGTGGCTTTAACCTTGGCTTGTT
  TCGGAACTCAACGGGAGGGTAACCATAAGCCAGGGACTGACTACCTTAAACCACCTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204674 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSKPKSLEIIGAPFSKGQPRGGVEKGPAALRKAGLLEKLKETEYDVRDHGDLAFVDVPNDSSFQIVKNP
  RSVGKANEELAGVVAEVQKNGRVSVVLGGDHSLAVGSISGHARVHPDLCVIWVDAHTDINTPLTTSSGNL
  HGQPVSFLLKELKGKFPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYIIKTLGIKYFSMTEVDKLGIGK
  VMEETFSYLLGRKKRPIHLSFDVDGLDPAFTPATGTPVLGGLSYREGLYITEEIYKTGLLSGLDIMEVNP
  TLGKTAEEVKSTVNTAVALTLACFGTQREGNHKPGTDYLKPPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007482
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007482.2, NP_031508.1
• RefSeq Size: 1489 bp
• RefSeq ORF: 972 bp
• Locus ID: 11846
• UniProt ID: Q61176
• Cytogenetics: 10 A4
• MW: 34.8 kDa
• Gene Summary: Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.[UniProtKB/Swiss-Prot Function]