Ankmy2 (BC012275) Mouse Tagged ORF Clone

CAT#: MR204185

Ankmy2 (Myc-DDK-tagged) - Mouse ankyrin repeat and MYND domain containing 2 (cDNA clone MGC:18754 IMAGE:4010781)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankmy2
• Synonyms: ZMYND20, MGC18754
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204185 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCACATAAAGAAAGGCGAGCTGACCCAGGAGGAAAAGGAACTGCTGGAAGTGATCGGGAAAGGTA
  CTGTCCAAGAAGCCGGAAGACTGTTGTCTAGTAAGAATGTTCATGTCAACTGCTTGGATGAGAATGGAAT
  GACTCCTTTGATGCATGCAGCCTATAAAGGGAAACTCGAGATGTGCAAGCTCCTCCTGCGCCATGGAGCT
  GACGCCAGTTGCCATCAGCATGAGCACGGGTACACAGCCCTCATGTTTGCTGCGCTTTCCGGTAATAAAG
  ACATCACTTGGGTTATGCTGGAAGCAGGCGCAGAGACCGATGTTGTGAACTCGGTGGGAAGAACTGCTGC
  CCAGATGGCAGCCTTTGTGGGTCAGCACGATTGCGTGGCTATCATCAACAATTTCTTCCCTCGAGAGAGA
  CTGGATTATTACACTAAACCCCAGGGACTGGATAAAGAGCCAAAACTGCCTCCAAAGTTGGCAGGCCCGC
  TACACAAAATCATCACCACAACGAATCTCCACCCTGTCAAGATCGTGATGCTTGTGAGTGAGAACCCTCT
  GCTGGCAGACGCAGCTGCTCTCGGTAAATGCTACAGGGTGATGGATTTGATCTGTGAGAAATGCATGAAA
  CAGAGAGACATGAATGAAGTGTTGGCTATGAAAATGCACTACATCAGCTGCATCTTTCAGAAATGCATTA
  CCTTCCTCAAAGAAGGAGAGAATAAGCTGGACACCCTGATCAGAAGCTTGTTGAAAGGCCGAGCGTCTGA
  TGGCTTCCCGGTGTATCAGGAAAAGATCATCAGAGAAAGCATCCGGAAGTTTCCATACTGTGAAGCCACA
  CTCCTACAGCAGCTGGTGCGAAGCATCGCTCCAGTTGAAATTGTAAGGCTCCTCTTTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204185 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGHIKKGELTQEEKELLEVIGKGTVQEAGRLLSSKNVHVNCLDENGMTPLMHAAYKGKLEMCKLLLRHGA
  DASCHQHEHGYTALMFAALSGNKDITWVMLEAGAETDVVNSVGRTAAQMAAFVGQHDCVAIINNFFPRER
  LDYYTKPQGLDKEPKLPPKLAGPLHKIITTTNLHPVKIVMLVSENPLLADAAALGKCYRVMDLICEKCMK
  QRDMNEVLAMKMHYISCIFQKCITFLKEGENKLDTLIRSLLKGRASDGFPVYQEKIIRESIRKFPYCEAT
  LLQQLVRSIAPVEIVRLLFC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC012275
• ORF Size: 900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012275, AAH12275
• RefSeq Size: 1525 bp
• RefSeq ORF: 902 bp
• Locus ID: 217473
• Cytogenetics: 12 A3
• MW: 33.6 kDa
• Gene Summary: May be involved in the trafficking of signaling proteins to the cilia.[UniProtKB/Swiss-Prot Function]