Tsen54 (NM_029557) Mouse Tagged ORF Clone

SKU MG227588

Tsen54 (tGFP-tagged) - Mouse tRNA splicing endonuclease 54 homolog (S. cerevisiae) (Tsen54), (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Tsen54
• Synonyms: 0610034P02Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227588 representing NM_029557; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCCCGAACCCGAACCCGGGTCGGTGGAGGTTCCCGCGGGGCGTGTGCTCAGCGCCTCGGAGCTCC; GCGCCGCGCGCTCGCGGTCCCAGAAGCTACCACAGCGGTCGCATGGCCCCAAGGACTTCCTCCCGGACGG; CTCGGAGGCCCAGGCGGAGCGGCTGCGCCTGTGTCGCCAAGAGCTGTGGCAGCTGCTGGCGGAGGAGCGC; GTGGAGCGCCTGGGCAGCTTGGTGGCCGCGGAGTGGAAACCAGAAGAGGGCTTTGTGGAGTTGACGTCTC; CTGCGGGGAAATTCTGGCAGACCATGGGCTACTCAGAGGAAGGACGGCAGCGACTTCACCCCGAAGAGGC; TTTGTATCTGCTGGAGTGTGGTTCGATTCAGCTCTTCTACCAAGACCTCCCACTGTCTATCCAAGAGGCA; TACCAGCTGCTGCTGACAGAGGACACCTTGAGTTTCCTGCAGTACCAGGTCTTCAGCCACCTGAAGAGAC; TGGGCTATGTGGTTCGTCGGTTCCAGCTAAGCTCTGTCGTGTCACCTTACGAGCGGCAGCTTAATTTGGA; TGGTTATGCCCAGTGCCTGGAGGATGGGTCTGGCAAGAGGAAGAGGAGTTCCAGCTGTCGGTCTGTTAAT; AAGAAGCCCAAGGTCCTGCAGAACTCCCTGCCGCCCGTCAGCCTGGCAGCCTCCAGCTCACCTGCCTGTG; ACCAGAGTAGCCAATACCCAGAGGAGAAGTCTCAGGACTCAAGCCCCAGGCAGGGCTCAGAGCTCCCCTT; GCAGTTCCTGGGGTCCTCAGAGCCTTGCTCTGATCTGGCCAGGGAAGACGTGGGGTGTGACCGAGAGAGT; CACAAAATAGAGAATGGAGCTAAGGGAACCCCTAAGCTACGCTGGAACTTTGAGCAGATCTCATTCCCCA; ACATGGCCTCTGATAGTCGCCACACTTTCCTGCCTGCTCCAGCCCCAGAGCTGCTCCCAGCCAATGTCAT; TGGGCGAGGGACAGATGCTGAGTCCTGGTGCCAAAAGCTGAACCAGCGGAGGGAGAAGCTCTCCCGTAGA; GACCGGGAACAGCAAGCAGTGGTCCAGCAGTTCCGGGAGGATGTTAACGCAGATCCCGAGGTGCGGGGCT; GCTCCAGCTGGCAGGAGTACAAGGAGCTGCTGCAGAGGCGACAGACGCAGAAGAGCCAGCCCCGGCCTCC; ACACCTATGGGGCCAGTCCGTTACCCCCTTGCTCGACCCTGATAAAGCCGATTGTCCAGCCGCGGTCCTG; CAACATATCTCTGTACTGCAGACGACACACCTTGCTGATGGAGGTTACCGGCTGCTGGAGAAGTCTGGAG; GCTTGCAGATCAGCTTCGATGTTTACCAGGCTGACGCTGTGGCGACGTTCCGAAAAAACAGCCCTGGCAA; ACCCTATGTGCGAATGTGCATTAGTGGGTTTGATGACCCTGTCCCAGACCTCTGCAGCCTCAAGTGCTTG; ACCTACCAGAGTGGGGATGTTCCCCTGATCTTTGCTCTAGTGGACCACGGTGACATCTCCTTCTACAGCT; TCAGAGACTTCACACTGCCCAGGGATCTGGGCCAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227588 representing NM_029557; Red=Cloning site Green=Tags(s); ; MEPEPEPGSVEVPAGRVLSASELRAARSRSQKLPQRSHGPKDFLPDGSEAQAERLRLCRQELWQLLAEER; VERLGSLVAAEWKPEEGFVELTSPAGKFWQTMGYSEEGRQRLHPEEALYLLECGSIQLFYQDLPLSIQEA; YQLLLTEDTLSFLQYQVFSHLKRLGYVVRRFQLSSVVSPYERQLNLDGYAQCLEDGSGKRKRSSSCRSVN; KKPKVLQNSLPPVSLAASSSPACDQSSQYPEEKSQDSSPRQGSELPLQFLGSSEPCSDLAREDVGCDRES; HKIENGAKGTPKLRWNFEQISFPNMASDSRHTFLPAPAPELLPANVIGRGTDAESWCQKLNQRREKLSRR; DREQQAVVQQFREDVNADPEVRGCSSWQEYKELLQRRQTQKSQPRPPHLWGQSVTPLLDPDKADCPAAVL; QHISVLQTTHLADGGYRLLEKSGGLQISFDVYQADAVATFRKNSPGKPYVRMCISGFDDPVPDLCSLKCL; TYQSGDVPLIFALVDHGDISFYSFRDFTLPRDLGH; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029557
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_029557.1, NP_083833.1
• RefSeq Size: 1972 bp
• RefSeq ORF: 1578 bp
• Locus ID: 76265
• UniProt ID: Q8C2A2
• Cytogenetics: 11 E2
• Summary: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).[UniProtKB/Swiss-Prot Function]