Trpv1 (NM_001001445) Mouse Tagged ORF Clone

SKU MG227160

Trpv1 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily V member 1 (Trpv1), (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Trpv1
• Synonyms: OTRPC1; TRPV1alpha; TRPV1beta; VR-1; Vr1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227160 representing NM_001001445; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGAAATGGGCTAGCTTAGACTCGGATGAATCTGAGCCCCCAGCCCAAGAGAACTCCTGCCCGGACC; CTCCAGACAGAGACCCTAACTCCAAGCCGCCTCCAGCCAAGCCCCACATCTTTGCTACCAGGAGTCGCAC; CCGGCTTTTTGGGAAGGGTGACTCAGAAGAGGCCTCTCCCATGGACTGCCCTTATGAGGAAGGCGGGCTG; GCCTCCTGCCCTATCATCACCGTCAGCTCTGTTGTCACTCTCCAGAGGTCTGTGGATGGACCTACCTGTC; TCAGGCAGACATCCCAGGACTCTGTCTCCACTGGTGTTGAGACGCCCCCAAGGCTCTATGATCGCAGGAG; CATCTTCGACGCTGTGGCTCAGAGCAACTGCCAGGAGCTGGAGAGCCTGCTGTCCTTCCTGCAGAAGAGC; AAGAAGCGCCTGACTGACAGCGAGTTCAAAGACCCAGAGACGGGAAAGACCTGTCTGCTCAAAGCCATGC; TCAATCTGCACAATGGGCAGAACGACACCATTGCTCTGCTCCTGGACATTGCCCGGAAGACAGATAGCCT; GAAGCAGTTTGTCAATGCCAGCTACACAGACAGCTACTACAAGGGCCAGACAGCATTACACATTGCCATT; GAAAGGCGGAACATGGCACTGGTGACCCTCTTGGTGGAGAATGGAGCAGATGTCCAGGCTGCTGCTAACG; GGGACTTCTTCAAGAAAACCAAAGGGAGGCCTGGCTTCTACTTTGGTGAGCTGCCCCTGTCCCTGGCTGC; GTGCACCAACCAGCTGGCCATTGTGAAGTTCCTGCTGCAGAACTCCTGGCAGCCTGCAGACATCAGTGCA; CGGGATTCGGTGGGCAACACGGTGCTGCACGCCCTTGTGGAGGTGGCAGATAACACAGCTGACAACACCA; AGTTCGTGACAAACATGTACAACGAGATCCTGATCCTGGGGGCCAAACTCCACCCCACACTGAAGCTAGA; AGAACTCACCAACAAGAAGGGGCTTACACCGCTGGCTCTGGCTGCCAGCAGTGGGAAGATTGGGGTCTTG; GCCTACATTCTCCAGAGGGAGATCCACGAACCAGAGTGCCGGCACCTGTCCAGGAAGTTCACTGAATGGG; CCTATGGGCCCGTGCACTCCTCCCTTTATGACCTGTCCTGCATTGACACCTGTGAGAAGAATTCAGTGCT; GGAGGTGATCGCCTACAGTAGCAGTGAGACCCCCAACCGCCACGACATGCTTCTCGTGGAGCCCTTGAAC; CGACTCCTGCAGGACAAGTGGGACAGATTTGTCAAGCGCATCTTCTACTTCAACTTCTTCGTCTACTGCT; TGTATATGATCATCTTCACCACGGCTGCTTACTATCGGCCTGTGGAAGGCTTGCCCCCCTATAAGCTGAA; TAACACCGTTGGGGACTATTTCCGTGTCACTGGAGAGATCCTGTCTGTGTCAGGAGGAGTCTACTTCTTC; TTCCGAGGGATCCAGTATTTCCTGCAGAGGCGACCATCCCTCAAGAGTTTGTTTGTGGACAGCTACAGTG; AGATACTTTTCTTTGTACAGTCACTGTTCATGCTGGTGTCTGTGGTACTGTACTTCAGCCATCGCAAGGA; GTATGTGGCTTCCATGGTGTTCTCCCTGGCCATGGGCTGGACCAACATGCTCTACTACACCCGAGGATTC; CAGCAGATGGGCATCTATGCTGTCATGATTGAGAAGATGATCCTCAGAGACCTGTGTCGGTTTATGTTCG; TCTACCTCGTGTTCTTGTTTGGATTTTCCACAGCCGTAGTGACACTGATCGAGGATGGGAAGAATAACTC; ACTGCCTGTGGAGTCCCCACCACACAAGTGTCGGGGATCTGCCTGCAGGCCAGGTAACTCTTACAACAGC; CTGTATTCCACATGTCTGGAGCTGTTCAAGTTCACCATCGGCATGGGTGACCTGGAGTTCACCGAGAACT; ATGACTTCAAGGCTGTCTTCATCATCCTGTTACTGGCCTATGTGATTCTCACCTACATCCTCCTGCTCAA; CATGCTCATTGCTCTCATGGGCGAGACTGTCAACAAGATTGCACAAGAGAGCAAGAACATCTGGAAGCTG; CAGCGAGCCATCACCATCCTGGATACAGAGAAGAGTTTCCTGAAGTGCATGAGGAAGGCCTTCCGCTCCG; GCAAGCTGCTGCAGGTGGGGTTCACGCCGGACGGCAAGGATGACTTCCGGTGGTGCTTCAGGGTGGATGA; GGTGAACTGGACTACCTGGAACACCAACGTGGGCATCATCAACGAGGACCCAGGCAACTGTGAGGGCGTC; AAGCGCACCCTGAGCTTCTCCCTGCGGTCAGGCCGAGTTTCAGGGAGAAACTGGAAGAACTTTGCCCTGG; TTCCCCTTCTGAGGGACGCAAGCACTCGAGATAGGCATAGCACCCAGCCGGAAGAAGTTCAGCTGAAGCA; CTATACGGGATCCCTTAAGCCAGAGGATGCTGAGGTCTTCAAGGATTCCATGGCCCCAGGGGAGAAA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227160 representing NM_001001445; Red=Cloning site Green=Tags(s); ; MEKWASLDSDESEPPAQENSCPDPPDRDPNSKPPPAKPHIFATRSRTRLFGKGDSEEASPMDCPYEEGGL; ASCPIITVSSVVTLQRSVDGPTCLRQTSQDSVSTGVETPPRLYDRRSIFDAVAQSNCQELESLLSFLQKS; KKRLTDSEFKDPETGKTCLLKAMLNLHNGQNDTIALLLDIARKTDSLKQFVNASYTDSYYKGQTALHIAI; ERRNMALVTLLVENGADVQAAANGDFFKKTKGRPGFYFGELPLSLAACTNQLAIVKFLLQNSWQPADISA; RDSVGNTVLHALVEVADNTADNTKFVTNMYNEILILGAKLHPTLKLEELTNKKGLTPLALAASSGKIGVL; AYILQREIHEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN; RLLQDKWDRFVKRIFYFNFFVYCLYMIIFTTAAYYRPVEGLPPYKLNNTVGDYFRVTGEILSVSGGVYFF; FRGIQYFLQRRPSLKSLFVDSYSEILFFVQSLFMLVSVVLYFSHRKEYVASMVFSLAMGWTNMLYYTRGF; QQMGIYAVMIEKMILRDLCRFMFVYLVFLFGFSTAVVTLIEDGKNNSLPVESPPHKCRGSACRPGNSYNS; LYSTCLELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKL; QRAITILDTEKSFLKCMRKAFRSGKLLQVGFTPDGKDDFRWCFRVDEVNWTTWNTNVGIINEDPGNCEGV; KRTLSFSLRSGRVSGRNWKNFALVPLLRDASTRDRHSTQPEEVQLKHYTGSLKPEDAEVFKDSMAPGEK; ; TRTRPLE - GFP Tag - V
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001445
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001001445.2
• RefSeq Size: 2520 bp
• RefSeq ORF: 2520 bp
• Locus ID: 193034
• UniProt ID: Q704Y3
• Cytogenetics: 11 45.25 cM
• Summary: Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli (PubMed:15194687, PubMed:15489017). Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia (PubMed:10764638). Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]