Pecam1 (NM_008816) Mouse Tagged ORF Clone

SKU MG227044

Pecam1 (tGFP-tagged) - Mouse platelet/endothelial cell adhesion molecule 1 (Pecam1) transcript variant 1, (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Pecam1
• Synonyms: C85791; Cd31; Pecam; PECAM-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227044 representing NM_008816; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTCCTGGCTCTGGGACTCACGCTGGTGCTCTATGCAAGCCTCCAGGCTGAGGAAAACTCCTTCACCA; TCAACAGCATCCATATGGAAAGCCTGCCATCATGGGAGGTGATGAATGGGCAGCAACTGACCCTGGAGTG; CCTTGTGGACATCAGCACCACCTCGAAAAGCAGGTCTCAGCACCGGGTGCTGTTCTATAAGGACGATGCG; ATGGTGTATAACGTCACCTCCAGGGAGCACACCGAGAGCTACGTCATTCCTCAGGCTCGGGTCTTCCACT; CCGGGAAGTACAAATGCACAGTGATGCTGAACAACAAGGAAAAAACCACGATTGAGTACGAGGTGAAGGT; GCATGGCGTATCCAAGCCCAAGGTGACACTGGACAAAAAGGAGGTGACAGAAGGCGGGGTCGTGACGGTC; AATTGTTCCTTGCAAGAAGAAAAGCCACCGATCTTTTTTAAAATTGAAAAATTAGAAGTGGGGACAAAGT; TTGTCAAGCGAAGGATAGATAAGACCTCCAACGAGAACTTTGTGCTCATGGAATTCCCCATTGAGGCGCA; GGACCACGTGTTAGTGTTTCGCTGCCAAGCTGGGATCCTGTCCGGATTCAAATTGCAGGAGTCAGAACCC; ATCAGGAGTGAATACGTCACCGTGCAGGAGTCCTTCTCCACTCCCAAGTTTGAAATCAAGCCCCCTGGGA; TGATCATAGAAGGGGACCAGCTGCACATTAGGTGCATAGTTCAAGTGACACACTTGGTCCAGGAGTTTAC; AGAAATTATCATCCAAAAAGACAAGGCGATTGTAGCCACCTCCAAGCAAAGCAGTGAAGCTGTCTACTCA; GTCATGGCCATGGTCGAGTACAGTGGACACTACACCTGCAAAGTGGAATCAAACCGTATCTCCAAAGCCA; GTAGCATCATGGTCAACATAACAGAGCTGTTTCCCAAGCCGAAGTTAGAGTTCTCCTCCAGTCGTCTGGA; CCAAGGGGAGTTGTTGGACCTGTCCTGCTCCGTCTCGGGCACACCTGTAGCCAACTTCACCATCCAGAAG; GAAGAGACGGTCTTGTCGCAGTATCAGAATTTCAGCAAGATCGCCGAGGAGAGTGACAGCGGGGAGTACA; GCTGTACTGCAGGCATCGGCAAAGTGGTCAAGAGAAGCGGCCTGGTACCGATCCAGGTGTGCGAAATGCT; CTCGAAGCCCAGTATTTTTCATGATGCCAAGTCTGAGATCATAAAAGGACATGCCATAGGCATCAGCTGC; CAGTCCGAAAATGGAACTGCACCCATCACTTACCACCTTATGAAAGCAAAGAGTGACTTCCAGACTCTCG; AGGTGACCTCCAATGACCCAGCAACATTCACAGATAAGCCCACCAGAGACATGGAATACCAGTGCAGAGC; GGATAATTGCCATTCCCACCCCGCGGTGTTCAGCGAGATCCTGAGGGTCAGGGTGATAGCCCCAGTGGAT; GAAGTTGTGATTTCCATCCTGTCGAGTAACGAGGTACAGTCTGGAAGTGAAATGGTACTTCGGTGCTCTG; TGAAAGAGGGGACGAGCCCAATCACGTTTCAGTTTTACAAAGAAAAGGAGGACAGACCCTTCCACCAAGC; GGTCGTGAATGACACCCAAGCGTTTTGGCACAACAAACAAGCTAGCAAGAAGCAGGAAGGACAGTACTAC; TGTACAGCCTCCAACAGAGCCAGCAGTATGAGGACCAGTCCCCGAAGCAGCACTCTTGCAGTCAGAGTCT; TCCTTGCCCCATGGAAGAAAGGGCTCATTGCGGTGGTTGTCATTGGAGTGGTCATCGCCACCTTAATAGT; TGCAGCCAAATGCTACTTCCTGAGGAAAGCCAAGGCCAAACAGAAACCCGTGGAGATGTCCAGGCCAGCT; GCTCCACTTCTGAACTCCAACAGCGAGAAGATTTCTGAGCCTAGTGTGGAAGCCAACAGCCATTACGGTT; ATGATGATGTTTCTGGAAATGATGCAGTAAAACCCATAAATCAAAATAAAGACCCCCAGAACATGGATGT; AGAATACACAGAAGTGGAAGTGTCCTCCCTTGAGCCTCACCAAGCTCTGGGAACGAGAGCCACAGAGACG; GTGTACAGTGAGATCCGGAAGGTCGACCCTAAGAACGGAAGGCTCCCT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227044 representing NM_008816; Red=Cloning site Green=Tags(s); ; MLLALGLTLVLYASLQAEENSFTINSIHMESLPSWEVMNGQQLTLECLVDISTTSKSRSQHRVLFYKDDA; MVYNVTSREHTESYVIPQARVFHSGKYKCTVMLNNKEKTTIEYEVKVHGVSKPKVTLDKKEVTEGGVVTV; NCSLQEEKPPIFFKIEKLEVGTKFVKRRIDKTSNENFVLMEFPIEAQDHVLVFRCQAGILSGFKLQESEP; IRSEYVTVQESFSTPKFEIKPPGMIIEGDQLHIRCIVQVTHLVQEFTEIIIQKDKAIVATSKQSSEAVYS; VMAMVEYSGHYTCKVESNRISKASSIMVNITELFPKPKLEFSSSRLDQGELLDLSCSVSGTPVANFTIQK; EETVLSQYQNFSKIAEESDSGEYSCTAGIGKVVKRSGLVPIQVCEMLSKPSIFHDAKSEIIKGHAIGISC; QSENGTAPITYHLMKAKSDFQTLEVTSNDPATFTDKPTRDMEYQCRADNCHSHPAVFSEILRVRVIAPVD; EVVISILSSNEVQSGSEMVLRCSVKEGTSPITFQFYKEKEDRPFHQAVVNDTQAFWHNKQASKKQEGQYY; CTASNRASSMRTSPRSSTLAVRVFLAPWKKGLIAVVVIGVVIATLIVAAKCYFLRKAKAKQKPVEMSRPA; APLLNSNSEKISEPSVEANSHYGYDDVSGNDAVKPINQNKDPQNMDVEYTEVEVSSLEPHQALGTRATET; VYSEIRKVDPKNGRLP; ; TRTRPLE - GFP Tag - V
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008816
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008816.3
• RefSeq Size: 3305 bp
• RefSeq ORF: 2151 bp
• Locus ID: 18613
• UniProt ID: Q08481
• Cytogenetics: 11 E1
• Summary: Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).[UniProtKB/Swiss-Prot Function]