Pnpla2 (NM_001163689) Mouse Tagged ORF Clone

SKU MG226260

Pnpla2 (tGFP-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2) transcript variant 1, (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Pnpla2
• Synonyms: 0610039C21Rik; 1110001C14Rik; Atgl; TTS-2.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226260 representing NM_001163689; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTCCCGAGGGAGACCAAGTGGAACATCTCATTCGCTGGCTGCGGCTTCCTCGGGGTCTACCACATTG; GCGTGGCCTCCTGCCTCCGTGAGCACGCGCCCTTCCTGGTGGCCAACGCCACTCACATCTACGGAGCCTC; GGCAGGGGCGCTCACCGCCACAGCGCTGGTCACTGGGGCCTGCCTGGGTGAAGCAGGTGCCAACATTATT; GAGGTGTCCAAGGAGGCCCGGAAGCGGTTCCTGGGTCCTCTGCATCCCTCCTTCAACCTGGTGAAGACCA; TCCGTGGCTGTCTACTAAAGACCCTGCCTGCTGATTGCCATGAGCGCGCCAATGGACGCCTGGGCATCTC; CCTGACTCGTGTTTCAGACGGAGAGAACGTCATCATATCCCACTTTAGCTCCAAGGATGAGCTCATCCAG; GCCAATGTCTGCAGCACATTTATCCCGGTGTACTGTGGCCTCATTCCTCCTACCCTCCAAGGGGTGCGCT; ATGTGGATGGCGGCATTTCAGACAACTTGCCACTTTATGAGCTGAAGAATACCATCACAGTGTCCCCATT; CTCAGGCGAGAGTGACATCTGCCCTCAGGACAGCTCCACCAACATCCACGAGCTTCGCGTCACCAACACC; AGCATCCAGTTCAACCTTCGCAATCTCTACCGCCTCTCGAAGGCTCTCTTCCCGCCAGAGCCCATGGTCC; TCCGAGAGATGTGCAAACAGGGCTACAGAGATGGACTTCGATTCCTTAGGAGGAATGGCCTACTGAACCA; ACCCAACCCTTTGCTGGCACTGCCCCCAGTTGTCCCCCAGGAAGAGGATGCAGAGGAAGCTGCTGTGGTG; GAGGAGAGGGCTGGAGAGGAGGATCAATTGCAGCCTTATAGAAAAGATCGAATTCTAGAGCACCTGCCTG; CCAGACTCAATGAGGCCCTGCTGGAGGCCTGTGTGGAACCAAAGGACCTGATGACCACCCTTTCCAACAT; GCTACCAGTGCGCCTGGCAACGGCCATGATGGTGCCCTATACTCTGCCGCTGGAGAGTGCAGTGTCCTTC; ACCATCCGCTTGTTGGAGTGGCTGCCTGATGTCCCTGAAGATATCCGGTGGATGAAAGAGCAGACGGGTA; GCATCTGCCAGTATCTGGTGATGAGGGCCAAGAGGAAATTGGGTGGCCATCTGCCTTCCAGACTGTCTGA; GCAGGTGGAACTGCGACGTGCCCAGTCTCTGCCCTCTGTGCCACTGTCTTGCGCCACCTACAGTGAGGCC; CTACCCAACTGGGTACGAAACAACCTCTCACTGGGGGACGCGCTGGCCAAGTGGGAAGAATGCCAGCGTC; AGCTACTGCTGGGTCTCTTCTGCACCAATGTGGCCTTCCCGCCGGATGCCTTGCGCATGCGCGCACCTGC; CAGCCCCACTGCCGCAGATCCTGCCACCCCACAGGATCCACCTGGCCTCCCGCCTTGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226260 representing NM_001163689; Red=Cloning site Green=Tags(s); ; MFPRETKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEAGANII; EVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGENVIISHFSSKDELIQ; ANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNT; SIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNGLLNQPNPLLALPPVVPQEEDAEEAAVV; EERAGEEDQLQPYRKDRILEHLPARLNEALLEACVEPKDLMTTLSNMLPVRLATAMMVPYTLPLESAVSF; TIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGGHLPSRLSEQVELRRAQSLPSVPLSCATYSEA; LPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPATPQDPPGLPPC; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163689
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001163689.1, NP_001157161.1
• RefSeq Size: 2635 bp
• RefSeq ORF: 1461 bp
• Locus ID: 66853
• UniProt ID: Q8BJ56
• Cytogenetics: 7 F5
• Summary: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.[UniProtKB/Swiss-Prot Function]