Golga2 (NM_001080968) Mouse Tagged ORF Clone

SKU MG224240

Golga2 (tGFP-tagged) - Mouse golgi autoantigen golgin subfamily a 2 (Golga2) transcript variant 2, (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Golga2
• Synonyms: AW555139; GM130; mKIAA4150
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224240 representing NM_001080968; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTGGCCCCCCCGCTTCCCCCCTCCCCGCCCCGGGATGTCGGAAGAAACCAGGCAGAGCAAATTGGCTG; CGGCCAAGAAAAAGCTGAGAGAGTATCAGCAGAAGAACAGCCCGGGTGTTCCTGCAGGAGCAAAGAAGAA; GAAAAAGATTAAAAATGGCCATAGCCCAGAGAGACCCACTGCCAGTGACTGTCAGTCACCAGAGAATGTG; CCCACAGACCATATTGCTCCTGCACCGCCAACCGCTGCTACTGACACTATGTTTCTTGGTGTCACCCCTT; CCCCTGATGCTGACCTCACTCAGAGCCATGATGCTGGCAATTGCTCTAACCTCATGGAGGAGACCAAGAC; TTTCTCATCGACTGAGAGTCTGCGACAACTTTCTCAACAGCTCAATGGCCTTGTGTCTGAGTCTACATCC; TACATCAACGGGGAGGGCCTCACATCTTCCAACATGAAGGAACTGGAGAACCGGTACCAAGAGCTAGCAG; TAGCCCTGGACTCCAGCTATGTAACAAACAAACAACTCAGTAGCACGATAGAGGAATTGAAACAACAGAA; CCAAGACACTCTGGATCAACTGGAGAAAGAGAAGAAGGATTATCAGCAGAAGCTGGCAAAGGAGCAGGGC; TCTCTCAGGGAACAGCTGCAGGTTCACATTCAGACCATAGGCATCCTGGTGTCTGAGAAGGCAGAATTAC; AGACGGCCCTGGCCCACACTCAGCAAGCAGCCAGGCAGAAAGCAGGAGAGTCGGAGGATCTTGCCAGCCG; ACTACAGTCCTCCCGACAGCGTGTGGGAGAGCTGGAGCGGACACTGTCTACCGTGTCAACGCAACAGAAA; CAGGCAGACAGGTATAACAAGGACCTAACCAAAGAGCGGGATGCCCTCAAGCTGGAGCTGTATAAGAACA; GCAAAAGCAATGAGGACCTGAGGCAGCAGAACTCAGAACTGGAAGAGAAACTCCGAGTCCTGGTGGCAGA; GAAAGCAGCTGCACAGTTGGGGGTGGAGGAGCTGCAGAAGAAGTTAGAGATGTCAGAGCTGCTGCTGCAG; CAGTTTTCTAGTCAGTCTTCGGCCGCAGGCGGTAATGAGCAGTTACAACATGCCATGGAGGAGCGGGCTC; AGCTGGAGACCCATGTTAGCCAGCTGATGGAATCACTGAAGCAGCTCCAGGTGGAGAGAGACCAGTATGC; GGAGAACCTAAAAGGAGAGAGTGCCATGTGGCAGCAGAGGGTACAGCAAATGGCGGAGCAGGTGCATACA; CTGAAGGAAGAGAAGGAGCACAGAGAACGGCAGGTACAAGAGCTGGAGACCAGCTTGGCCGCACTCCGGA; GTCAGATGGAGGAGCCACCGCCTCCAGAGCCTCCAGCTGGGCCCTCCGAGGCGGAAGAGCAGCTGCAGGG; AGAGGTCGAGCAGCTGCACAAGGAACTGGAGAGGCTGACGGGACAGCTACGGGCTCAGGTGCAGGACAAT; GAGAGCCTGAGCCACCTCAACCGGGAGCAGGAGGGGCGTCTGCTGGAGCTGGAGCGGGAGGCCCAGCGCT; GGAGCGAGCAGGCCGAGGAGCGCAAGCAGATCCTGGAGAGCATGCAGAGTGACCGCACCACCATCAGCAG; AGCACTGTCACAGAACCGAGAGCTGAAGGAGCAGCTGGCCGAGCTGCAGAACGGCTTCGTCAGGCTGACC; AATGAGAACATGGAGATCACCAGTGCGCTGCAGTCAGAGCAGCATGTCAAGAAAGAGCTAGCCCGGAAGC; TGGGAGAGCTGCAGGAGAGACTGGGAGAGCTGAAGGAGACGGTGGAGCTCAAGAGCCAAGAAGCACAGGG; TCTACAGGAGCAGCGAGACCAGTGCCTGTCCCACCTGCAGCAGTACGCTGCTGCTTACCAACAGCACTTG; GCGGCCTATGAGCAGCTGACCTCTGAGAAGGAGGCAATACACAAGCAGCTTCTGCTGCAGACTCAGCTCA; TGGACCAGCTGCAGCATGAGGAGGTACAGGGCAAGATGGCAGCCGAGCTGGCCCGCCAGGAGCTGCAGGA; GGCCCAGGAGCGCCTAAAAGCTACCAGCCAGGAGAACCAACAGCTCCAAGCCCAGCTGAGCCTCTTGGTC; CTCCCTGGGGAAGGAGATGTGGACCAGGAGGAAGAAGATGAGGAGGTTCCTCAGTCGAGTCTGGCCATCC; CAGAAGACCTAGACAGTAGAGAGGCCATGGTGGCATTCTTTAATGCCGCTATAGCCAGAGCAGAGGAAGA; ACAGGCCCGACTACGTGTGCAGCTGAAAGAGCAGAAGGCACGTTGCCGGAGTTTGTCTCACTTGGCAGCC; CCAGTCCAAAGCAAGCTTGAAAAGGAGGCAGTGGTCCCCAGGAATGTGGATGACTCTGCATCTGAGGAGA; GCAATCAGGCCCTACATGTAGCCATGGAGAAGCTGCAGAGCCGCTTCCTGGAGGTCATGCAGGAGAAAGT; GGAGCTCAAGGAGAGGGTAGAGGAGCTTGAACATTGCTGCATTCAGCTTTCTGGAGAGACAGACACCATT; GGAGAGTACATTGCCCTTTACCAAAATCAGAGGGCGGTGCTGAAGGCTCGGCATTTGGAGAAGGAAGAAT; ACATCAGCCGGCTGGCTCAGGACAAGGAGGAGATGAAGGTGAAGCTGCTGGAGCTCCAGGAGCTGGTGTT; GAGGCTTGTGAACGAGCGCAATGAATGGCAGGGCAAGTTCCTGGCCGTCTCTCAGAACCCTGGTGACGTG; CTCACCCCAGTGCCCACAGGCTCCCAGGAATTTGGTGCTGCTGACCAGCAGGATGACCTCAGGGAGGTGA; GCCTGGCTGACGATATAGAGCCTGCACAAGGAGAGGCAGGGGTACCTGCTCCCCATGAGAACCCTACTGC; ACAGCAGATCATGCAGCTGCTGCGTGAGATCCAGAACCCCCGGGAGCGGCCAGGCCTGGGTAGCAACCCT; TGCATCCCCTTTTTCTACCGTGCCGATGAGAACGACGAGGTGAAGATCATGGTTGTA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224240 representing NM_001080968; Red=Cloning site Green=Tags(s); ; MWPPRFPPPRPGMSEETRQSKLAAAKKKLREYQQKNSPGVPAGAKKKKKIKNGHSPERPTASDCQSPENV; PTDHIAPAPPTAATDTMFLGVTPSPDADLTQSHDAGNCSNLMEETKTFSSTESLRQLSQQLNGLVSESTS; YINGEGLTSSNMKELENRYQELAVALDSSYVTNKQLSSTIEELKQQNQDTLDQLEKEKKDYQQKLAKEQG; SLREQLQVHIQTIGILVSEKAELQTALAHTQQAARQKAGESEDLASRLQSSRQRVGELERTLSTVSTQQK; QADRYNKDLTKERDALKLELYKNSKSNEDLRQQNSELEEKLRVLVAEKAAAQLGVEELQKKLEMSELLLQ; QFSSQSSAAGGNEQLQHAMEERAQLETHVSQLMESLKQLQVERDQYAENLKGESAMWQQRVQQMAEQVHT; LKEEKEHRERQVQELETSLAALRSQMEEPPPPEPPAGPSEAEEQLQGEVEQLHKELERLTGQLRAQVQDN; ESLSHLNREQEGRLLELEREAQRWSEQAEERKQILESMQSDRTTISRALSQNRELKEQLAELQNGFVRLT; NENMEITSALQSEQHVKKELARKLGELQERLGELKETVELKSQEAQGLQEQRDQCLSHLQQYAAAYQQHL; AAYEQLTSEKEAIHKQLLLQTQLMDQLQHEEVQGKMAAELARQELQEAQERLKATSQENQQLQAQLSLLV; LPGEGDVDQEEEDEEVPQSSLAIPEDLDSREAMVAFFNAAIARAEEEQARLRVQLKEQKARCRSLSHLAA; PVQSKLEKEAVVPRNVDDSASEESNQALHVAMEKLQSRFLEVMQEKVELKERVEELEHCCIQLSGETDTI; GEYIALYQNQRAVLKARHLEKEEYISRLAQDKEEMKVKLLELQELVLRLVNERNEWQGKFLAVSQNPGDV; LTPVPTGSQEFGAADQQDDLREVSLADDIEPAQGEAGVPAPHENPTAQQIMQLLREIQNPRERPGLGSNP; CIPFFYRADENDEVKIMVV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080968
• ORF Size: 2997 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001080968.1, NP_001074437.1
• RefSeq Size: 4396 bp
• RefSeq ORF: 3000 bp
• Locus ID: 99412
• UniProt ID: Q921M4
• Cytogenetics: 2 B
• Summary: Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (PubMed:28028212). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (PubMed:28028212). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization (By similarity). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (By similarity). Regulates the meiotic spindle pole assembly, probably via the same mechanism (PubMed:21552007). Also regulates the centrosome organization (By similarity). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (By similarity).[UniProtKB/Swiss-Prot Function]