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Pdlim7 (NM_001114088) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216070
Pdlim7 (tGFP-tagged) - Mouse PDZ and LIM domain 7 (Pdlim7) transcript variant a, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdlim7
Synonyms LMP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216070 representing NM_001114088
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTCCTTCAAGGTGGTGCTGGAAGGGCCAGCCCCTTGGGGCTTCCGTCTGCAAGGGGGCAAGGACT
TCAATGTGCCCCTCTCTATCTCTCGGCTCACGCCCGGAGGCAAAGCTGCACAGGCCGGTGTGGCTGTGGG
AGACTGGGTACTGAATATTGACGGTGAGAACGCGGGCAGCCTCACGCACATCGAAGCCCAGAACAAGATC
CGCGCCTGTGGGGAGCGCCTCAGCCTGGGTCTTAGCAGAGCCCAGCCTGTTCAGAGCAAACCACAGAAGG
CCCTGACCCCTCCCGCCGACCCCCCGAGGTACACTTTTGCACCAAGCGCCTCCCTCAACAAGACGGCCCG
GCCCTTCGGGGCACCCCCACCTACTGACAGCACCCTGCGGCAGAATGGACAGTTGCTCAGACAGCCGGTC
CCCGATGCCAGCAAGCAGCGGCTGATGGAGGATACCGAAGACTGGCGGCCGCGGCCGGGGACAGGCCAGT
CCCGCTCCTTCCGCATCCTTGCCCACCTCACGGGCACAGAGTTCATGCAAGACCCGGATGAGGAATTCAT
GAAGAAGTCAAGCCAGGTGCCCAGGACAGAAGCCCCAGCCCCAGCCTCAACTATACCCCAGGAATCCTGG
CCTGGCCCCACCACTCCCAGCCCCACCAGCCGCCCACCCTGGGCTGTGGATCCTGCATTTGCTGAGCGCT
ATGCCCCAGACAAAACCAGCACAGTGCTGACCCGGCACAGCCAGCCAGCCACACCCACGCCTCTGCAGAA
CCGCACCTCCATAGTGCAGGCCGCAGCTGGAGGGGGCACAGGAGGGGGCAGCAACAACGGCAAGACTCCT
GTATGCCACCAGTGCCACAAGATCATCCGCGGCCGCTACCTGGTAGCACTGGGCCACGCATACCATCCCG
AGGAGTTTGTGTGCAGCCAGTGTGGAAAGGTCCTGGAAGAGGGTGGCTTCTTCGAGGAGAAGGGAGCTAT
CTTTTGCCCCTCCTGCTATGATGTGCGCTATGCACCCAACTGTGCCAAATGCAAGAAGAAGATCACTGGA
GAGATCATGCATGCTCTGAAGATGACCTGGCACGTCCATTGCTTCACCTGTGCTGCCTGCAAAACGCCAA
TTCGCAACAGAGCCTTTTACATGGAAGAAGGGGCCCCCTACTGCGAGCGAGACTATGAGAAGATGTTTGG
CACAAAATGTCGAGGCTGTGACTTCAAGATTGATGCTGGAGACCGCTTCCTGGAAGCGCTGGGCTTCAGC
TGGCATGACACATGCTTTGTTTGCGCAATATGTCAGATCAACTTGGAAGGAAAGACCTTCTACTCCAAGA
AGGACAAGCCCCTCTGCAAGAGCCACGCCTTCTCTCACGTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216070 representing NM_001114088
Red=Cloning site Green=Tags(s)

MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLNIDGENAGSLTHIEAQNKI
RACGERLSLGLSRAQPVQSKPQKALTPPADPPRYTFAPSASLNKTARPFGAPPPTDSTLRQNGQLLRQPV
PDASKQRLMEDTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEFMKKSSQVPRTEAPAPASTIPQESW
PGPTTPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQNRTSIVQAAAGGGTGGGSNNGKTP
VCHQCHKIIRGRYLVALGHAYHPEEFVCSQCGKVLEEGGFFEEKGAIFCPSCYDVRYAPNCAKCKKKITG
EIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGAPYCERDYEKMFGTKCRGCDFKIDAGDRFLEALGFS
WHDTCFVCAICQINLEGKTFYSKKDKPLCKSHAFSHV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001114088
ORF Size 1371 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001114088.2, NP_001107560.1
RefSeq Size 2636 bp
RefSeq ORF 1374 bp
Locus ID 67399
UniProt ID Q3TJD7
Cytogenetics 13 B1
Summary May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001114088" in other vectors (4)
SKU Description Size Price
MC216151 Pdlim7 (untagged) - Mouse PDZ and LIM domain 7 (Pdlim7), transcript variant a, (10ug) 10 ug
$503.00
MR216070 Pdlim7 (Myc-DDK-tagged) - Mouse PDZ and LIM domain 7 (Pdlim7), transcript variant a 10 ug
$289.00 $457.00
MR216070L3 Lenti ORF clone of Pdlim7 (Myc-DDK-tagged) - Mouse PDZ and LIM domain 7 (Pdlim7), transcript variant a 10 ug
$757.00
MR216070L4 Lenti ORF clone of Pdlim7 (mGFP-tagged) - Mouse PDZ and LIM domain 7 (Pdlim7), transcript variant a 10 ug
$757.00

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