Fzd6 (NM_008056) Mouse Tagged ORF Clone

SKU MG210188

Fzd6 (tGFP-tagged) - Mouse frizzled homolog 6 (Drosophila) (Fzd6)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Fzd6
• Synonyms: Fz6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210188 representing NM_008056; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAAGGTCCCCGTTTCTGTTGGCGTGCATTCTTCTGCCCCTCGTAAGAGGACACAGCCTTTTCACCT; GTGAGCCAATCACCGTTCCCAGATGTATGAAAATGACTTACAACATGACGTTCTTCCCTAACCTGATGGG; TCATTATGACCAGGGGATCGCTGCTGTGGAAATGGGGCACTTTCTGCATCTTGCAAATCTAGAATGTTCA; CCAAACATTGAAATGTTCCTTTGCCAAGCTTTTATACCAACCTGCACAGAGCAAATTCATGTAGTTCTAC; CCTGTCGGAAATTGTGTGAGAAAATAGTTTCTGATTGCAAAAAACTAATGGACACTTTTGGCATCCGATG; GCCTGAAGAACTTGAATGTAACAGATTGCCACACTGTGATGACACTGTTCCTGTAACTTCTCATCCACAC; ACAGAGCTTTCTGGGCCACAGAAGAAATCAGATCAAGTCCCAAGAGACATTGGATTTTGGTGTCCAAAGC; ACCTTAGGACTTCCGGGGACCAAGGCTATAGGTTTCTGGGAATTGAACAGTGTGCCCCTCCGTGCCCCAA; TATGTATTTTAAAAGTGATGAACTAGACTTTGCCAAAAGTTTCATAGGAATAGTTTCAATATTTTGTCTT; TGTGCAACTCTGTTCACGTTCCTTACATTTTTAATTGACGTTAGACGATTCAGATACCCAGAGAGACCAA; TTATCTATTACTCTGTCTGCTACAGCATTGTCTCTCTCATGTACTTCGTGGGGTTTTTGCTGGGCAATAG; CACGGCTTGTAATAAGGCAGACGAGAAGCTGGAGCTCGGGGACACCGTTGTCCTAGGGTCAAAGAATAAG; GCTTGCAGTGTGGTATTTATGTTTCTGTATTTTTTTACAATGGCTGGCACCGTGTGGTGGGTGATTCTCA; CCATTACGTGGTTCTTAGCTGCCGGGAGAAAATGGAGTTGCGAAGCTATTGAACAAAAAGCAGTGTGGTT; CCATGCCGTTGCCTGGGGGGCGCCCGGGTTCCTGACCGTCATGCTGCTCGCTATGAATAAGGTTGAAGGA; GACAACATTAGCGGCGTTTGCTTCGTTGGCCTGTATGACCTGGACGCCTCTCGCTACTTCGTCCTTCTGC; CTCTGTGCCTCTGCGTATTTGTTGGGCTGTCTCTCCTCTTAGCCGGCATCATCTCCTTGAATCATGTCCG; ACAAGTCATACAGCATGATGGCCGGAACCAAGAGAAGCTAAAGAAATTCATGATTCGCATCGGAGTCTTC; AGTGGCCTGTATCTTGTGCCCTTAGTGACACTTCTCGGTTGCTATGTCTATGAGCTAGTGAACAGGATCA; CCTGGGAGATGACATGGTTCTCTGATCATTGTCACCAGTACCGCATCCCGTGCCCTTACCAGGCAAATCC; AAAAGCTCGACCAGAATTGGCTTTATTTATGATAAAATATCTGATGACATTAATTGTTGGTATCTCTGCG; GTCTTCTGGGTTGGAAGCAAAAAGACGTGCACAGAATGGGCCGGGTTCTTTAAGCGAAACCGCAAGCGAG; ACCCCATCAGTGAGAGCCGCCGAGTGCTGCAAGAGTCCTGTGAGTTCTTCCTGAAGCACAACTCTAAAGT; GAAGCACAAGAAGAAGCATGGCGCACCAGGGCCCCATAGGCTGAAGGTCATTTCCAAGTCCATGGGAACT; AGCACAGGAGCGACCACAAATCATGGCACCTCTGCCATGGCAATCGCTGACCATGATTACTTAGGGCAAG; AAACTTCAACAGAAGTCCACACCTCCCCAGAAGCATCCGTCAAAGAGGGACGAGCAGACCGAGCAAACAC; TCCCAGCGCCAAAGATCGGGACTGTGGGGAACCTGCAGGGCCCAGCTCCAAGCTCTCTGGGGACCGGAAT; GGCAGGGAAAGCCGAGCGGGCGGCCTGAAGGAGAGAAGCAATGGATCAGAGGGGGCTCCAAGTGAAGGAA; GGGTAAGTCCAAAGAGCAGCGTTCCTGAGACTGGCCTGATAGACTGCAGCACTTCACAGGCCGCCAGTTC; TCCAGAACCAACCAGCCTCAAGGGCTCCACATCTCTGCCTGTTCACTCAGCTTCCAGAGCTAGGAAAGAG; CAGGGTGCTGGCAGCCATTCCGACGCT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210188 representing NM_008056; Red=Cloning site Green=Tags(s); ; MERSPFLLACILLPLVRGHSLFTCEPITVPRCMKMTYNMTFFPNLMGHYDQGIAAVEMGHFLHLANLECS; PNIEMFLCQAFIPTCTEQIHVVLPCRKLCEKIVSDCKKLMDTFGIRWPEELECNRLPHCDDTVPVTSHPH; TELSGPQKKSDQVPRDIGFWCPKHLRTSGDQGYRFLGIEQCAPPCPNMYFKSDELDFAKSFIGIVSIFCL; CATLFTFLTFLIDVRRFRYPERPIIYYSVCYSIVSLMYFVGFLLGNSTACNKADEKLELGDTVVLGSKNK; ACSVVFMFLYFFTMAGTVWWVILTITWFLAAGRKWSCEAIEQKAVWFHAVAWGAPGFLTVMLLAMNKVEG; DNISGVCFVGLYDLDASRYFVLLPLCLCVFVGLSLLLAGIISLNHVRQVIQHDGRNQEKLKKFMIRIGVF; SGLYLVPLVTLLGCYVYELVNRITWEMTWFSDHCHQYRIPCPYQANPKARPELALFMIKYLMTLIVGISA; VFWVGSKKTCTEWAGFFKRNRKRDPISESRRVLQESCEFFLKHNSKVKHKKKHGAPGPHRLKVISKSMGT; STGATTNHGTSAMAIADHDYLGQETSTEVHTSPEASVKEGRADRANTPSAKDRDCGEPAGPSSKLSGDRN; GRESRAGGLKERSNGSEGAPSEGRVSPKSSVPETGLIDCSTSQAASSPEPTSLKGSTSLPVHSASRARKE; QGAGSHSDA; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008056
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008056.2
• RefSeq Size: 4089 bp
• RefSeq ORF: 2130 bp
• Locus ID: 14368
• UniProt ID: Q61089
• Cytogenetics: 15 15.22 cM
• Summary: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism. Involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (By similarity). Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear.[UniProtKB/Swiss-Prot Function]