Atp6v1a (NM_007508) Mouse Tagged ORF Clone

SKU MG209449

Atp6v1a (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit A (Atp6v1a)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Atp6v1a
• Synonyms: AI647066; Atp6a1; Atp6a2; Atp6v1a1; VA68; VPP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209449 representing NM_007508; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATTTCTCCAAGCTACCCAAAATCCGCGATGAGGATAAAGAAAGTACATTTGGTTATGTGCATGGAG; TCTCAGGGCCTGTGGTTACAGCCTGTGACATGGCGGGCGCTGCCATGTACGAGCTGGTGAGAGTGGGGCA; CAGCGAGCTGGTTGGAGAAATTATTCGATTGGAAGGTGACATGGCCACCATTCAGGTGTATGAAGAGACT; TCTGGTGTCTCTGTTGGAGACCCCGTACTCCGCACTGGTAAACCTCTCTCGGTCGAGCTGGGTCCCGGGA; TTATGGGAGCCATTTTTGATGGTATACAGAGACCTCTGTCGGATATCAGCAGTCAGACCCAAAGTATCTA; CATCCCCAGAGGAGTCAATGTGTCTGCTCTCAGCAGAGATATCAAATGGGAGTTTATACCCAGCAAAAAC; CTACGGGTTGGTAGTCATATCACTGGTGGAGACATTTATGGGATTGTCAATGAGAACTCCCTCATCAAAC; ACAAAATCATGTTGCCCCCACGTAACAGAGGAAGCGTGACTTACATCGCGCCGCCTGGGAATTATGATGC; GTCCGATGTCGTCCTGGAGCTTGAGTTTGAAGGTGTGAAGGAGAAGTTCAGCATGGTCCAAGTGTGGCCT; GTGCGGCAGGTGCGGCCTGTCACTGAGAAGCTGCCCGCCAATCACCCCTTGCTTACTGGCCAGAGAGTCC; TCGATGCCCTTTTCCCGTGTGTTCAGGGAGGAACTACTGCTATCCCAGGCGCCTTTGGCTGTGGAAAGAC; TGTGATTTCCCAGTCTCTATCCAAGTACTCCAACAGTGACGTCATCATCTATGTCGGCTGCGGTGAGAGA; GGCAACGAGATGTCAGAAGTTCTCCGCGACTTCCCTGAGCTCACCATGGAGGTTGATGGGAAAGTAGAGT; CCATCATGAAGAGGACAGCGCTGGTAGCCAACACCTCCAACATGCCTGTGGCTGCAAGAGAGGCCTCCAT; CTACACTGGAATTACACTATCAGAATATTTCCGTGACATGGGCTACCACGTCAGTATGATGGCGGACTCT; ACCTCTAGATGGGCTGAGGCCCTCAGAGAAATCTCTGGTCGCTTAGCTGAGATGCCTGCAGATAGTGGAT; ACCCTGCATACCTTGGTGCCCGGCTGGCTTCTTTCTATGAGCGAGCAGGCAGAGTGAAATGTCTTGGAAA; CCCTGAGAGAGAAGGGAGTGTCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCA; GTCACTTCTGCAACTCTGGGTATTGTTCAGGTGTTCTGGGGCTTGGATAAGAAGTTAGCTCAGCGCAAGC; ACTTCCCCTCTGTCAACTGGCTCATCAGCTACAGCAAGTACATGCGTGCCCTGGACGAGTACTATGACAA; GCACTTCACGGAGTTTGTTCCTCTGAGGACCAAAGCTAAGGAGATCCTGCAGGAAGAAGAGGATCTGGCA; GAAATCGTGCAGCTCGTGGGAAAGGCCTCTTTAGCAGAGACGGATAAAATCACTCTGGAGGTAGCAAAAC; TTATTAAAGATGACTTCCTACAGCAAAATGGGTACACTCCTTATGACAGGTTCTGTCCATTCTACAAGAC; AGTGGGGATGCTGTCCAACATGATTTCATTCTATGACATGGCCCGCCGAGCTGTGGAGACCACTGCCCAG; AGTGACAATAAGATCACATGGTCCATTATCCGGGAGCACATGGGGGAGATTCTCTATAAACTCTCCTCCA; TGAAATTCAAGGATCCAGTGAAAGATGGCGAGGCAAAGATCAAGGCCGACTATGCACAGCTTCTTGAAGA; TATGCAGAATGCATTCCGTAGCCTTGAAGAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209449 representing NM_007508; Red=Cloning site Green=Tags(s); ; MDFSKLPKIRDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEET; SGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWEFIPSKN; LRVGSHITGGDIYGIVNENSLIKHKIMLPPRNRGSVTYIAPPGNYDASDVVLELEFEGVKEKFSMVQVWP; VRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGER; GNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADS; TSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDP; VTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLA; EIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMISFYDMARRAVETTAQ; SDNKITWSIIREHMGEILYKLSSMKFKDPVKDGEAKIKADYAQLLEDMQNAFRSLED; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007508
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007508.2
• RefSeq Size: 3947 bp
• RefSeq ORF: 1854 bp
• Locus ID: 11964
• UniProt ID: P50516
• Cytogenetics: 16 B4
• Summary: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.[UniProtKB/Swiss-Prot Function]