Ncbp3 (NM_025818) Mouse Tagged ORF Clone

SKU MG209440

1200014J11Rik (tGFP-tagged) - Mouse RIKEN cDNA 1200014J11 gene (1200014J11Rik)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ncbp3
• Synonyms: 1200014J11Rik; C78393; C130061O14Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209440 representing NM_025818; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCCGTCCGCGGCCTGCGAGTGTCTGTGAAGGCGGAGGCCCCCGCTGGGCCGGCTTTGGGGCTTC; CGTCGCCCGAGGTGGAGTCCGGTTTGGAGCGTGGCGAGCCGGAGCCCATGGAAGTGGAAGAAGGCGAGCT; GGAAATCGTGCCGGTGCGGCGCTCCCTAAAGGAACTGCTCCCGGACACAAGCAGAAGATATGAAAACAAG; GCTGGCAGCTTCATCACTGGGATCGATGTCACCTCCAAGGAAGCAATTGAAAAGAAAGAACAGCGAGCCA; AGCGCTTCCACTTTCGAGCAGAAGTAAATCTTGCCCAGAGAAATGTAGCCTTGGACCGAGACATGATGAA; GAAAGCAATTCCCAAGGTGAGACTGGAGACAATCTACATTTGTGGAGTGGATGAAATGAGCACCCAGGAT; ATCTTCTCCTATTTTAAGGAATATCCTCCAGCTCACATTGAGTGGTTGGATGACACCTCCTGCAATGTGG; TTTGGCTGGACGAGATGACGGCCACACGAGCTCTCATCAATATGAGTTCTCTGCCAGCCCAGGATAAGAT; GAGAAGCAGAGATGCCAGTGAAGACAAGTCGTCGGAGAAGAACAAGAAAGACAAGCAGGAAGACAGTTCA; GATGACGACGAGACTGAAGAAGGAGAAGTGGAAGATGAGAATTCAAGTGATGTAGAGTTGGACACGTTGT; CTCAGGTAGAAGAAGAGTCTCTGTTACGAAATGATCTTCGTCCAGCTAACAAACTTGCTAAAGGAAATAG; ACTATTCATGAGATTTGCTACAAAAGATGACAAAAAGGAACTTGGAGCAGCCAGAAGAAGTCAATACTAC; ATGAAGTATGGAAATCCAAATTATGGAGGCATGAAAGGAATTCTTAGCAATTCATGGAAGCGAAGATATC; ATTCCCGTCGCATTCAGCGGGATGTGATCAAGAAGAGAGCCCTGATTGGGGATGACGTTGGCTTGACGTC; GTATAAACACCGACATTCCGGATTAGTGAATGTTCCTGAGGAACCCATTGAGGAAGAGGAGGAGGAGGAG; GAGGAAGAGGAGGACCAGGACATGGATGCCGATGACAGAGTCGTGGTGGAGTACCATGAGGAGCTCCCAG; GTCTCAAGCAGCCCCGTGAGCGGAGCCTGTCTAGGCGATCCAGTGCGAGCAGCTCAGACTCAGATGAGAT; GGACTATGATCTGGAACTGAAGATGATTTCTACTCCCTCCCCAAAGAAAAGCATGAAAATGACCATGTAT; GCAGATGAAGTAGAGTCCCAACTGAAAAGTATTAGGAATCCCATGAGGGCAGATAGTATATCCACAAGCA; ATATAAAAAACCGAATTGGTAACAAATTGCCTCCTGAGAAATTTGCAGATGTCCGTCATCTCTTAGATGA; AAAGAGGCAGCATTCCTGTCCACGGCCAGCAGTCAGCAGTACTAAACCAGATATTCGCCAGCGGTTAGGA; AAAAGACCGTATTCTCCAGAGAAGGCTTTTAGTAGTAACCAGGTGGTTCGGAGAGAGCCCTCTTCAGATG; TACATAGTAGGCTAGGTGTTCCCAGGCAAGATGTTAAAGGCCTCTACTCTGATACTCGGGAGAGGAAATC; AGGTGGTTTGTGGACTCGCTTAGGATCTACACCCAAGACCAAAGAAAAGAACACGAAGAAAGTGGATCAC; AGGGCATCTGGTGCTGAGGAAGATGACTCGGAGCTTCAGAGAGCATGGGGTGCTCTGATCAAGGAGAAAG; AAGAGTCTCGCCAGAAGAAGAGTCGGCTGGATAGCTTGCCTTCGCTCCAGATTGAAGTCAGCCGGGAAAG; TAGCTCTGGTTCAGAGGCAGAGTCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209440 representing NM_025818; Red=Cloning site Green=Tags(s); ; MAAVRGLRVSVKAEAPAGPALGLPSPEVESGLERGEPEPMEVEEGELEIVPVRRSLKELLPDTSRRYENK; AGSFITGIDVTSKEAIEKKEQRAKRFHFRAEVNLAQRNVALDRDMMKKAIPKVRLETIYICGVDEMSTQD; IFSYFKEYPPAHIEWLDDTSCNVVWLDEMTATRALINMSSLPAQDKMRSRDASEDKSSEKNKKDKQEDSS; DDDETEEGEVEDENSSDVELDTLSQVEEESLLRNDLRPANKLAKGNRLFMRFATKDDKKELGAARRSQYY; MKYGNPNYGGMKGILSNSWKRRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEE; EEEEDQDMDADDRVVVEYHEELPGLKQPRERSLSRRSSASSSDSDEMDYDLELKMISTPSPKKSMKMTMY; ADEVESQLKSIRNPMRADSISTSNIKNRIGNKLPPEKFADVRHLLDEKRQHSCPRPAVSSTKPDIRQRLG; KRPYSPEKAFSSNQVVRREPSSDVHSRLGVPRQDVKGLYSDTRERKSGGLWTRLGSTPKTKEKNTKKVDH; RASGAEEDDSELQRAWGALIKEKEESRQKKSRLDSLPSLQIEVSRESSSGSEAES; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025818
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025818.3, NP_080094.3
• RefSeq Size: 6284 bp
• RefSeq ORF: 1848 bp
• Locus ID: 66874
• UniProt ID: Q8BZR9
• Cytogenetics: 11 B4
• Summary: Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858).[UniProtKB/Swiss-Prot Function]