Peli1 (NM_023324) Mouse Tagged ORF Clone
CAT#: MG206617
- TrueORF®
Peli1 (tGFP-tagged) - Mouse pellino 1 (Peli1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_023324" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Peli1 |
Synonyms | 2810468L03Rik; A930031K15Rik; AA409794; AI586297; D11Ertd676e |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206617 representing NM_023324
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTCTCCTGATCAAGAAAATCATCCTTCCAAAGCCCCAGTAAAATATGGCGAACTCATTGTCTTAG GATATAATGGATCTCTCCCAAACGGTGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACC TAAGGCAAATGGGGTGAAGCCTAGCACCGTGCACATTGCATGTACTCCTCAGGCTGCCAAGGCAATAAGC AACAAGGACCAGCATAGCATATCATATACTTTATCTCGAGCCCAGACGGTGGTGGTTGAATATACTCATG ACAGCAACACTGATATGTTTCAGATTGGTCGGTCAACTGAAAGTCCTATTGATTTTGTAGTAACTGACAC CGTTCCTGGAAGTCAGAGTAATTCCGACACGCAGTCAGTACAAAGCACTATATCAAGATTTGCCTGTAGG ATCATATGTGAGCGCAGTCCCCCCTTTACAGCTCGGATTTATGCTGCAGGGTTTGATTCATCAAAAAACA TCTTTCTTGGGGAGAAGGCTGCCAAGTGGAAGACATCTGATGGGCAGATGGATGGCTTGACCACTAATGG AGTTCTTGTGATGCATCCACGTAATGGGTTCACAGAAGACTCCAAACCTGGAATATGGAGAGAAATATCA GTATGTGGGAACGTCTTCAGTCTGCGTGAAACCAGATCAGCTCAGCAGAGAGGAAAGATGGTGGAAATTG AAACCAATCAGCTACAAGATGGCTCCTTAATTGACCTTTGTGGTGCAACCTTGCTGTGGCGTACTGCAGA AGGCCTTTCCCATACTCCTACTGTGAAGCACTTAGAAGCTTTAAGACAGGAGATCAATGCAGCTCGGCCG CAGTGCCCTGTAGGGTTCAACACACTAGCCTTCCCCAGTATGAAGAGGAAGGATGTTGTAGATGAAAAGC AACCATGGGTATATCTAAACTGCGGCCATGTCCATGGTTATCATAACTGGGGAAACAAAGAAGAACGTGA CGGCAAAGATCGTGAATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTCCCTCTGTGGCTTGGATGTGAA GCTGGATTTTATGTGGACGCCGGCCCTCCCACCCATGCCTTTAGCCCCTGTGGGCACGTGTGTTCAGAAA AGACAACGGCTTACTGGTCCCAGATCCCACTTCCTCATGGTACGCACACTTTTCATGCAGCCTGCCCCTT CTGTGCACATCAGTTGGCTGGTGAACAAGGCTATATCAGACTTATTTTCCAAGGACCTTTAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206617 representing NM_023324
Red=Cloning site Green=Tags(s) MFSPDQENHPSKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAIS NKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQSVQSTISRFACR IICERSPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREIS VCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLEALRQEINAARP QCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCE AGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_023324 |
ORF Size | 1254 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023324.2, NP_075813.2 |
RefSeq Size | 3501 bp |
RefSeq ORF | 1257 bp |
Locus ID | 67245 |
UniProt ID | Q8C669 |
Cytogenetics | 11 13.81 cM |
Gene Summary | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:16951688). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201212 | Peli1 (untagged) - Mouse pellino 1 (Peli1), (10ug) |
USD 457.00 |
|
MR206617 | Peli1 (Myc-DDK-tagged) - Mouse pellino 1 (Peli1) |
USD 457.00 |
|
MR206617L3 | Lenti ORF clone of Peli1 (Myc-DDK-tagged) - Mouse pellino 1 (Peli1) |
USD 757.00 |
|
MR206617L4 | Lenti ORF clone of Peli1 (mGFP-tagged) - Mouse pellino 1 (Peli1) |
USD 757.00 |
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