Peli1 (NM_023324) Mouse Untagged Clone

CAT#: MC201212

Peli1 (untagged) - Mouse pellino 1 (Peli1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Peli1
• Synonyms: 2810468L03Rik; A930031K15Rik; AA409794; AI586297; D11Ertd676e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC016515 sequence for NM_023324
  ATTTGAGAAAGACAGCCAAGCTCATGTTTTCTCCTGATCAAGAAAATCATCCTTCCAAAGCCCCAGTAAA ATATGGCGAACTCATTGTCTTAGGATATAATGGATCTCTCCCAAACGGTGATAGAGGAAGGAGGAAAAGT AGGTTTGCTTTGTTTAAAAGACCTAAGGCAAATGGGGTGAAGCCTAGCACCGTGCACATTGCATGTACTC CTCAGGCTGCCAAGGCAATAAGCAACAAGGACCAGCATAGCATATCATATACTTTATCTCGAGCCCAGAC GGTGGTGGTTGAATATACTCATGACAGCAACACTGATATGTTTCAGATTGGTCGGTCAACTGAAAGTCCT ATTGATTTTGTAGTAACTGACACCGTTCCTGGAAGTCAGAGTAATTCCGACACGCAGTCAGTACAAAGCA CTATATCAAGATTTGCCTGTAGGATCATATGTGAGCGCAGTCCCCCCTTTACAGCTCGGATTTATGCTGC AGGGTTTGATTCATCAAAAAACATCTTTCTTGGGGAGAAGGCTGCCAAGTGGAAGACATCTGATGGGCAG ATGGATGGCTTGACCACTAATGGAGTTCTTGTGATGCATCCACGTAATGGGTTCACAGAAGACTCCAAAC CTGGAATATGGAGAGAAATATCAGTATGTGGGAACGTCTTCAGTCTGCGTGAAACCAGATCAGCTCAGCA GAGAGGAAAGATGGTGGAAATTGAAACCAATCAGCTACAAGATGGCTCCTTAATTGACCTTTGTGGTGCA ACCTTGCTGTGGCGTACTGCAGAAGGCCTTTCCCATACTCCTACTGTGAAGCACTTAGAAGCTTTAAGAC AGGAGATCAATGCAGCTCGGCCGCAGTGCCCTGTAGGGTTCAACACACTAGCCTTCCCCAGTATGAAGAG GAAGGATGTTGTAGATGAAAAGCAACCATGGGTATATCTAAACTGCGGCCATGTCCATGGTTATCATAAC TGGGGAAACAAAGAAGAACGTGACGGCAAAGATCGTGAATGTCCTATGTGTAGGTCTGTTGGTCCCTATG TCCCTCTGTGGCTTGGATGTGAAGCTGGATTTTATGTGGACGCCGGCCCTCCCACCCATGCCTTTAGCCC CTGTGGGCACGTGTGTTCAGAAAAGACAACGGCTTACTGGTCCCAGATCCCACTTCCTCATGGTACGCAC ACTTTTCATGCAGCCTGCCCCTTCTGTGCACATCAGTTGGCTGGTGAACAAGGCTATATCAGACTTATTT TCCAAGGACCTTTAGACTAGCAGCTGTCCTCCAGGACTGCATTGTAAGTTTATAAGCTAAGTGATTGGGT TTGCTAACCGACCCCTTGTCCATGTAAGTTTCTCTGCTCTGGTCATTTGCATTAAGATGAAGAATTTTTT AAATATTTATAAGCAATTTCTGAGAAAAATCTGGGAAACTCAAGCAAAGGAATTTTTGAAAGTTCCAGAC TTCTGAACTCTGAGTTTTGAAAATCTATTTTGAGAAAAAAAAGACATAGTTTAATTTGATGCCTTCCTTT GTTTAGTGTTCTTGAATCACCGCCCACCCTCAGTGTTGAGAGGTTGTGCTGTAGAACTGAGGGTCTGTTG GTTCAAACTATGTTAGTTTACAATTTGTTGCAGACATTGTAAAATACAGCAACATGTATATTAATTTTTC TATTTATCTTTATCATAGAAAGTACCTTAGAATGTTGTGATAGGCTAGCATGGTAATGATGGTGTCACAC ATTTGGTGTGAGTGGTAGGGTAGTGAGCACAAGGCTCAAAGGTTTGCAAAGTTAAGGAGAAAGACAGGAG AGCTTCCCCCCCCCCCAGCCCCATAATTAGGGGATATTTCATAAAACCAGAATATTTCATAAAACCAGAT TCATATTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023324
• Insert Size: 1257 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016515, AAH16515
• RefSeq Size: 1912 bp
• RefSeq ORF: 1257 bp
• Locus ID: 67245
• UniProt ID: Q8C669
• Cytogenetics: 11 13.81 cM
• Gene Summary: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:16951688). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (By similarity).[UniProtKB/Swiss-Prot Function]