Peli1 (NM_023324) Mouse Tagged ORF Clone

CAT#: MR206617

Peli1 (Myc-DDK-tagged) - Mouse pellino 1 (Peli1)

Product Images

Circular map for MR206617

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Peli1
• Synonyms: 2810468L03Rik; A930031K15Rik; AA409794; AI586297; D11Ertd676e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206617 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTCTCCTGATCAAGAAAATCATCCTTCCAAAGCCCCAGTAAAATATGGCGAACTCATTGTCTTAG
  GATATAATGGATCTCTCCCAAACGGTGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACC
  TAAGGCAAATGGGGTGAAGCCTAGCACCGTGCACATTGCATGTACTCCTCAGGCTGCCAAGGCAATAAGC
  AACAAGGACCAGCATAGCATATCATATACTTTATCTCGAGCCCAGACGGTGGTGGTTGAATATACTCATG
  ACAGCAACACTGATATGTTTCAGATTGGTCGGTCAACTGAAAGTCCTATTGATTTTGTAGTAACTGACAC
  CGTTCCTGGAAGTCAGAGTAATTCCGACACGCAGTCAGTACAAAGCACTATATCAAGATTTGCCTGTAGG
  ATCATATGTGAGCGCAGTCCCCCCTTTACAGCTCGGATTTATGCTGCAGGGTTTGATTCATCAAAAAACA
  TCTTTCTTGGGGAGAAGGCTGCCAAGTGGAAGACATCTGATGGGCAGATGGATGGCTTGACCACTAATGG
  AGTTCTTGTGATGCATCCACGTAATGGGTTCACAGAAGACTCCAAACCTGGAATATGGAGAGAAATATCA
  GTATGTGGGAACGTCTTCAGTCTGCGTGAAACCAGATCAGCTCAGCAGAGAGGAAAGATGGTGGAAATTG
  AAACCAATCAGCTACAAGATGGCTCCTTAATTGACCTTTGTGGTGCAACCTTGCTGTGGCGTACTGCAGA
  AGGCCTTTCCCATACTCCTACTGTGAAGCACTTAGAAGCTTTAAGACAGGAGATCAATGCAGCTCGGCCG
  CAGTGCCCTGTAGGGTTCAACACACTAGCCTTCCCCAGTATGAAGAGGAAGGATGTTGTAGATGAAAAGC
  AACCATGGGTATATCTAAACTGCGGCCATGTCCATGGTTATCATAACTGGGGAAACAAAGAAGAACGTGA
  CGGCAAAGATCGTGAATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTCCCTCTGTGGCTTGGATGTGAA
  GCTGGATTTTATGTGGACGCCGGCCCTCCCACCCATGCCTTTAGCCCCTGTGGGCACGTGTGTTCAGAAA
  AGACAACGGCTTACTGGTCCCAGATCCCACTTCCTCATGGTACGCACACTTTTCATGCAGCCTGCCCCTT
  CTGTGCACATCAGTTGGCTGGTGAACAAGGCTATATCAGACTTATTTTCCAAGGACCTTTAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206617 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFSPDQENHPSKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAIS
  NKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQSVQSTISRFACR
  IICERSPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREIS
  VCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLEALRQEINAARP
  QCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCE
  AGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023324
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023324.2, NP_075813.2
• RefSeq Size: 3501 bp
• RefSeq ORF: 1257 bp
• Locus ID: 67245
• UniProt ID: Q8C669
• Cytogenetics: 11 13.81 cM
• MW: 46.3 kDa
• Gene Summary: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:16951688). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (By similarity).[UniProtKB/Swiss-Prot Function]