Fam20b (NM_145413) Mouse Tagged ORF Clone

SKU MG206431

Fam20b (tGFP-tagged) - Mouse RIKEN cDNA C530043G21 gene (C530043G21Rik)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Fam20b
• Synonyms: C530043G21Rik; mKIAA0475
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206431 representing NM_145413; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGCTGAAGCAGCGAGTTGTGCTGTTAGCAATACTCCTCGTCATTTTTATCTTCACCAAAGTTTTCC; TGATAGACAATTTAGATACATCAGCTGCCAACCGAGAGGACCAGAGGGCTTTTCACCGAATGATGACTGG; CTTGCGGGTGGAGCTGGTGCCCAAGTTGGACCATACCCTGCAGTCTCCTTGGGAGATTGCAGCCCAGTGG; GTGGTGCCCCGGGAAGTGTATCCTGAAGAGACACCAGAGCTGGGAGCAATCATGCATGCCATGGCCACTA; AGAAAATCATTAAAGCTGACGTGGGCTATAAAGGGACACAGCTAAAAGCTTTACTGATTCTTGAAGGAGG; ACAGAAAGTTGTCTTTAAGCCTAAGCGGTACAGCAGAGACTATGTGGTAGAAGGGGAGCCATACGCTGGT; TATGACAGACACAATGCAGAGGTGGCGGCCTTCCATTTGGACAGGATTCTGGGTTTCCGCCGCGCTCCCT; TGGTGGTGGGCAGATATGTTAATCTGCGGACAGAAGTTAAGCCTGTTGCCACGGAGCAGCTGCTGAGCAC; CTTCCTAACTGTAGGGAACAATACTTGTTTCTATGGGAAGTGCTACTACTGCCGAGAAACAGAGCCAGCA; TGTGCTGACGGTGACATGATGGAGGGCTCTGTCACACTTTGGCTTCCAGATGTGTGGCCTCTGCAGAAAC; ATCGACATCCCTGGGGCAGGACCTACCGAGAAGGCAAACTGGCCAGGTGGGAATATGATGAGAGCTACTG; CGATGCTGTGAAGAAAACATCCCCCTATGACTCAGGCCCGCGTCTCCTGGACATCATTGACACGGCTGTC; TTTGATTACTTGATTGGCAATGCTGATCGCCATCACTACGAGAGCTTTCAAGATGATGAAGGCGCGAGCA; TGCTTATTCTTCTTGATAATGCCAAAAGCTTTGGAAACCCCTCGCTGGATGAGAGAAGCATTCTTGCCCC; TCTCTATCAGTGTTGCATCATTCGGGTTTCAACCTGGAATAGACTGAATTATCTAAAGAATGGAGTACTA; AAGTCTGCCTTAAAATCTGCCATGGCCCATGACCCCATCTCCCCTGTGCTCTCCGATCCACACTTGGACA; CTGTGGACCAGCGGCTTCTGAATGTCTTGGCCACCATCAAGCAGTGTACTGACCAGTTTGGGACGGATAC; TGTGCTGGTGGAAGACAGGATGCCACTCTCCCACTTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206431 representing NM_145413; Red=Cloning site Green=Tags(s); ; MKLKQRVVLLAILLVIFIFTKVFLIDNLDTSAANREDQRAFHRMMTGLRVELVPKLDHTLQSPWEIAAQW; VVPREVYPEETPELGAIMHAMATKKIIKADVGYKGTQLKALLILEGGQKVVFKPKRYSRDYVVEGEPYAG; YDRHNAEVAAFHLDRILGFRRAPLVVGRYVNLRTEVKPVATEQLLSTFLTVGNNTCFYGKCYYCRETEPA; CADGDMMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAV; FDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVL; KSALKSAMAHDPISPVLSDPHLDTVDQRLLNVLATIKQCTDQFGTDTVLVEDRMPLSHL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145413
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145413.4, NP_663388.1
• RefSeq Size: 4238 bp
• RefSeq ORF: 1230 bp
• Locus ID: 215015
• UniProt ID: Q8VCS3
• Cytogenetics: 1 G3
• Summary: Responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature GAG chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-phosphorylation may influence the catalytic activity of B3GAT3 (GlcAT-I) which completes the precursor tetrasaccharide of GAG-protein linkage regions on which the repeating disaccharide region is synthesized.[UniProtKB/Swiss-Prot Function]