Cx3cl1 (NM_009142) Mouse Tagged ORF Clone

SKU MG206171

Cx3cl1 (tGFP-tagged) - Mouse chemokine (C-X3-C motif) ligand 1 (Cx3cl1)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Cx3cl1
• Synonyms: AB030188; ABCD-3; AI848747; CX3C; Cxc3; D8Bwg0439e; Scyd1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206171 representing NM_009142; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTCCCTCGCCGCTCGCGTGGCTGCTGCGCCTGGCCGCGTTCTTCCATTTGTGTACTCTGCTGCCGG; GTCAGCACCTCGGCATGACGAAATGCGAAATCATGTGCGACAAGATGACCTCACGAATCCCAGTGGCTTT; GCTCATCCGCTATCAGCTAAACCAGGAGTCCTGCGGCAAGCGTGCCATTGTCCTGGAGACGACACAGCAC; AGACGCTTCTGTGCTGACCCGAAGGAGAAATGGGTCCAAGACGCCATGAAGCATCTGGATCACCAGGCTG; CTGCCCTCACTAAAAATGGTGGCAAGTTTGAGAAGCGGGTGGACAATGTGACACCTGGGATCACCTTGGC; CACTAGGGGACTGTCCCCATCTGCCCTGACAAAGCCTGAATCCGCCACATTGGAAGACCTTGCTTTGGAA; CTGACTACTATTTCCCAGGAGGCCAGGGGGACCATGGGGACTTCCCAAGAGCCACCGGCAGCAGTGACCG; GATCATCTCTCTCAACTTCCGAGGCACAGGATGCAGGGCTTACGGCTAAGCCTCAGAGCATTGGAAGTTT; TGAGGCGGCTGACATCTCCACCACCGTTTGGCCGAGTCCTGCTGTCTACCAATCTGGATCTAGCTCCTGG; GCTGAGGAAAAAGCTACTGAGTCCCCCTCCACTACAGCCCCATCTCCTCAGGTGTCCACTACTTCACCTT; CAACCCCAGAGGAAAATGTTGGGTCCGAAGGCCAACCCCCATGGGTCCAGGGACAGGACCTCAGTCCAGA; GAAGTCTCTAGGGTCTGAGGAGATAAACCCAGTTCATACTGATAATTTCCAGGAGAGGGGGCCTGGCAAC; ACAGTCCACCCCTCAGTGGCTCCCATCTCCTCTGAAGAGACCCCCAGCCCAGAGCTGGTGGCCTCGGGCA; GCCAGGCTCCTAAGATAGAGGAACCCATCCATGCCACTGCAGATCCCCAGAAACTGAGTGTGCTTATCAC; TCCTGTCCCCGACACCCAGGCAGCCACAAGGAGGCAGGCAGTGGGGCTACTGGCTTTCCTTGGTCTTCTT; TTCTGCCTAGGGGTGGCCATGTTTGCTTACCAGAGCCTTCAGGGCTGTCCCCGCAAAATGGCGGGGGAAA; TGGTAGAAGGCCTCCGCTACGTCCCCCGTAGCTGTGGCAGTAACTCATACGTCCTGGTGCCAGTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206171 representing NM_009142; Red=Cloning site Green=Tags(s); ; MAPSPLAWLLRLAAFFHLCTLLPGQHLGMTKCEIMCDKMTSRIPVALLIRYQLNQESCGKRAIVLETTQH; RRFCADPKEKWVQDAMKHLDHQAAALTKNGGKFEKRVDNVTPGITLATRGLSPSALTKPESATLEDLALE; LTTISQEARGTMGTSQEPPAAVTGSSLSTSEAQDAGLTAKPQSIGSFEAADISTTVWPSPAVYQSGSSSW; AEEKATESPSTTAPSPQVSTTSPSTPEENVGSEGQPPWVQGQDLSPEKSLGSEEINPVHTDNFQERGPGN; TVHPSVAPISSEETPSPELVASGSQAPKIEEPIHATADPQKLSVLITPVPDTQAATRRQAVGLLAFLGLL; FCLGVAMFAYQSLQGCPRKMAGEMVEGLRYVPRSCGSNSYVLVPV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009142
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_009142.3, NP_033168.2
• RefSeq Size: 3144 bp
• RefSeq ORF: 1188 bp
• Locus ID: 20312
• UniProt ID: O35188
• Cytogenetics: 8 46.79 cM
• Summary: Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (By similarity). The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9177350, PubMed:10382755).[UniProtKB/Swiss-Prot Function]