Rp23 (BC061195) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200123
Rp23 (tGFP-tagged) - Mouse hypothetical protein LOC69357 (cDNA clone MGC:74336 IMAGE:6706856)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rp23
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200123 representing BC061195
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAAGCAACTAAGAAGACACACTATCTGACACAGGATGTTGTGCCATCAACTTCGGCATCCAAAC
CCAAGACAGTGAAGATTTGCCATGGTCAGCCCTGTTCCAAATGTAACAAGAAGATAAGAAACCCCAAGGT
GCAGGAGCCTAAGAAGAAGAGCAAGAAAACACTGCCAAGCATGCCTAAAAGAAAGACTTCGAAGAAAACT
ACACCACCAAAAACAAAGGACCAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200123 representing BC061195
Red=Cloning site Green=Tags(s)

MVKATKKTHYLTQDVVPSTSASKPKTVKICHGQPCSKCNKKIRNPKVQEPKKKSKKTLPSMPKRKTSKKT
TPPKTKDQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC061195
ORF Size 234 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC061195.1
RefSeq Size 746 bp
RefSeq ORF 236 bp
Locus ID 69357
Cytogenetics X C3
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Documents
Resources
"BC061195" in other vectors (4)
SKU Description Size Price
MC207114 Rp23 (untagged) - Mouse hypothetical protein LOC69357 (cDNA clone MGC:74336 IMAGE:6706856), (10ug) 10 ug
$165.00
MR200123 Rp23 (Myc-DDK-tagged) - Mouse hypothetical protein LOC69357 (cDNA clone MGC:74336 IMAGE:6706856) 10 ug
$289.00
MR200123L3 Lenti ORF clone of Rp23 (Myc-DDK-tagged) - Mouse hypothetical protein LOC69357 (cDNA clone MGC:74336 IMAGE:6706856) 10 ug
$450.00
MR200123L4 Lenti ORF clone of Rp23 (mGFP-tagged) - Mouse hypothetical protein LOC69357 (cDNA clone MGC:74336 IMAGE:6706856) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.