Shroom3 (NM_001077595) Mouse Untagged Clone

SKU MC229666

Shroom3 (untagged) - Mouse shroom family member 3 (Shroom3), transcript variant 2

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Shroom3
• Synonyms: AL022960; D5Ertd287e; Shrm; Shrm3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229666 representing NM_001077595; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGCAGATATCTCAGGGCACAATGGGCCCTCCTTGGCACCAGAGCTACCATTCCAGCTCTTCGACCA; GTGACCTCTCCAACTACGACCATGCTTATCTGAGGCGGAGCCCTGACCAGTGCAGCTCCCAGGGGAGCAT; GGAAAGCCTGGAGCCCAGTGGCGGATACCCACCCTGCCATCTCCTTTCTCCTGCCAAGTCCACGAGCAGC; ATCGACCAGCTAGGCCACCTTCATAACAAAAGGGACTCCGCATATAGCTCCTTCTCCACCAGTTCGAGCA; TCTTCGAGTACCCACCCCCTGGTGGCTCTGCCCGAGAACGTTCGGGTTCCATGGACGTGATTTCTGCCCG; GGGTGGCCTCCTAGAAGGGATGAGGCAGGCAGACATCCGCTACGTCAAGACGGTCTATGATACCCGGAGA; GGAGTATCTTCAGAGTATGAGGTGAACCCTTCAGCCTTGCTCCTTCAAGGTAGGGATGCCCATGCCTCAG; CCGACAGTCAGGGCTGTGCTAAATGGCACAGCATTCCTCGGGGCAAGGGAACGCCTTCCCCGTCTTGGAG; CCAGCAGTGTTCTGGTTCCCTGGAGACTGCTACTGACAACCTGCCCCAGAAGGCAGGCGCACCCCTGCCC; CCCACCCGCAGTGATAGCTATGCAGCCTTCCGCCATCGCGAGCGTCCCAGCTCCTGGTCCAGCCTTGATC; AGAAAAGGTTCTGCCGGCCTCAGACAAACTCTTCAGGCTCCCAGAAAACCCCATTCGCTGAGGACCAGCT; GCATACAGTCCCAGAGAGGAGTCCAGAGAACAGCCCCCCAGTGAAATCTAAGCATAATTATACCCAGAAG; GCGCAGCCTGGCCAACCTCTGCTGCCCACTGGCATCTATCCGGTACCTTCTCCGGAGCCTCACTTTGCCC; AGGTGCCCCAGCCCTCTGTGAGTAGTAATGGCACCGTCTACCCTGCGCTAGTCAAAGAGAGTGGATACAC; AGCTGCTCAGGGAACCTGTAACAAGATGGCTACCTTAGATGAGAACGGGAATCAAAATGAAGCTAGCAGG; CCTGGGTTTGCCTTCTGTCAGCCCCTAGAACACAACTCGGTGACTCCGGTGGAGAAAAGGCCAGAACCCA; CGGCCAAATACATCTACAAAGTCCATTTTTCTTCAGTGCCTGAAAATGAAGACTCCTCCCTAAAGAGGCA; CATCACACCTCCCCACGGCCATAGTCCATATCCCAGCGAGAGAAAGAACATCCACGGTGGCAGCAGAGCG; TGTTCCAATCACCACAGTCTCTCGTCCCCCCAGGCCCAGGCCCTGCACGTGGGTGATGACAGGAGGCCTT; CCAGGCTCTCTCAGCCCTGGGAGGGTGACTTTCAGGAAGATCACAATGCCAACCTCAGGCAGAAAGTGGA; GAGGGAGGGCCAAGGCCAGGGCCTGTCGGGCAACTCTGGCAGGACCAGATCGGCCTTCTCATCTCTGCAG; AATATTCCAGAGAGTCTGAGAAGACAAAGCAACGTGGAGCTTGGAGAAGCCCAGGAGGTTCACCCCGGGG; GCAGGTCCAAGGTGGAGGACCCTGGAAGGAAAGCAGGGGCTTCAGACATCAGGGGCTACCTGGACAGATC; TGTTTCCTATCCGAGGCCCGAGGGGAAAATGAACGCCGTAGACTCCGTCCACAGTGCAGACTCCAGGTAT; GAGGAGTCCCCGGCCCCAGCGCTGCCACAGACATCCGGTGCGAGCCAGCGGCGGCTCAGCTCCAGCAGCT; CCGCCGCCCCGCAGTACAGGAAGCCCCATTGCTCCGTGCTGGAGAAGGTTTCTAGAATAGAGGAACGAGA; GCAGGGGCGCCACAGACCCCTGAGCGTGGGCAGCTCTGCATACGGCCCCGGCTACAGGCCTGGGAGGACC; GGCCCGACCCCCAGTACTTCCAGCAGTGACCTCGACGATCCAAAAGCCGGCAGCGTCCATTTCTCGGAGT; CCACTGAACACCTGCGCAATGGGGAGCAGAACCCCCCAAACGGGGAGGCTAAGCAGGAAGAGGCTTCCCG; GCCCCAGTGCAGCCATTTGATCCGGAGGGCCCCTGCGGACGGCCGTGGACCTCCTGCCCGAGGCGGCGAG; CCCTCAAGGCCGGAGGCCCGCCTGCTCCGCAGCCAGAGCACCTTTCAGCTCTACAGTGAGGCGGAGAGGG; AAGCCTCGTGGTCAGAGGACAGGCCCGGTACCCCTGAGTCGCCCCTGCTCGATGCGCCTTTCAGCCGCGC; CTACAGAAACAGCATCAAGGATGCCCAGTCCCGCGTCCTGGGGGCCACGTCGTTTCGTCGCCGAGACCTG; GAACCGGGCACCCCCGCCACCTCGAGGCCCTGGCGCCCGCGACCCGCCTCCGCCCACGTGGGGATGCGGA; GCCCCGAGGCCGCGGTGCCCTCGTCCTCCCCGCACACCCCTCGGGAGCGACACAGCGTGACCCCGGCCGC; GCCCCAGGCTGCCCGCCGAGGCCCACGCCGGCGCCTCACTGTCGAGCAGAAGAAGCGCTCCTACTCGGAG; CCCGAGAAGATGAACGAGGTAGGGGTCTCGGAGGAGGCCGAGCCCACGCCCTGCGGCCCGCCGAGGCCCG; CGCAGCCGCGCTTCTCGGAGAGCACCGTGGCCGACCGGCGCCGCATCTTCGAGCGCGACGGCAAGGCCTG; CTCCACGCTCAGCCTGTCGGGCCCCGAGCTCAAGCAGTTCCAGCAGAGCGCCCTGGCCGACTACATCCAG; CGTAAGACGGGCAAGCGGCCCACAGGAGCCGCCTGCACACCGGAGGCCGGGCTGCGCGAGCGCGCACAGA; GCGCCTACCTCCAGGCCGGCCCCGCGGCGCCCGATGGCCCCGGCCTGGCCTCTGCCTGTAGCCTGAGCTC; CCTGCGAGAGCCCGAGGCGCTGCCCCGCAAGGAGCACACTCATCCGTCGGCCGCGGACGGCCCGCAGGCG; CCCCGTGATCGCAGCAGCTCCTTCGCCAGCGGACGCCTAGTCGGGGAGCGACGTCGCTGGGACCCTCAGG; TCCCCAGGCAGCTGCTCAGCGGAGCGAACTGCGAACCGAGGGGCGTCCAGAGGATGGACGGGGCCCCGGG; CGGGCCGCCTTCCTGGGGCATGGTGGCCGGGAAGGCCGGGAAGTCCAAGTCAGCCGAGGATCTGCTGGAG; CGCTCCGACACACTGGCGGTCCCTGTGCATGTGAGGTCCAGGTCGTCTCCCACCTCCGACAAGAAAGGTC; AGGATGTGCTCCTGAGGGAAGGCAGCAACTTTGGTTTTGTAAAGGATCCATGCTGTTTGGCTGGCCCTGG; ACCTAGGTCTCTCAGCTGCTCAGACAAAGGCCAGAATGAGCTGGCATTGCCCCTGCACCACCCTACGCCC; TGCTGGAACGGCTCAGGCTGCAAAGCCACTGTGGCTTCCTCAGCTCCTCCCGAATCTTCTGGAGCCGCGG; ATCATCTGAAGCAACGTAGAGCGCCTGGTCCTAGGCCACTCTCAGCAGGAATGCACGGCCACTTCCCAGA; CGCCAGAGCTGCCTCACTCAGCTCCCCTCTGCCATCACCTGTCCCCTCCAGTTACCGGTCACAGCTTGCC; ATGGATCAGCAGACTGGGCAACAGCCTCCCTCATCCCCTGCCTCTGCAGTGACCCAGCCTACCAGTCCAA; GAAGCCCTGAGCTCAGCAGCCCAGCATATGGGCTAGGGGAGGGGATGTGGAAGAGGACATCCCTGCCTCA; GCGACCCCCTCCCCCTTGGGTGAAATGGGCCCACGCGGTCAGAGAGGACGGCCTTGCTGAGGACACCTTG; GCGCCTGAGTTTGCGAACCTGAAGCACTATAGAAACCAGCCGAGTCGTCCGAGTTCCTGCAGCACTTCTG; ACCCAGACACTCCAGGAAGGATCTCGCTCCGAATATCGGAGTCAGCCCTTCAACCTTCCCCACCGCCCCG; GGGGGACTATGATGACGAAGTGTTCATGAAGGACTTGCACCCCAAGGTTACTTCAAGCCCCACATTTGAA; GCTCTTCCCCCACCCCCGCCACCTTCGCCACCGAGCGAGGAACCTCTAGTGAATGGCACAGATGACTTTC; CTCCGCCTCCTCCTCCCCAAGCCCTGTGTGAGGTACTGCTGGATGGTGAGGCATCCACGGAAGCTGGCAG; CGGCCCCTGCAGAATTCCCAGGGTGATGGTCACAAGGGAAGGGCACGTGCCTGGGGCAGCCCATTCCGAA; GGTAGTCAGATAATGACTGCCACACCACCCCAAACCTCGGCCAAAGGTTCAGAAGCCGAGTCCAACACGC; CTTCCAGCGCGAGCGCCCAGCCTCAGCTCAACGGTTCTCCTGGCAAGCAGCTCTGTCCCAGCCAGACCCG; AAACCTCACTTATGAACCTGTCGAGAGAACTCAGGATCTAGGAAAGAAAACTCACGCCGAGCCTCAGAAG; ACCTCAGAAGATATCCGGACAGAGGCTCTAGCCAAGGAAATTGTCCACCAAGACAAGTCTCTGGCAGACA; TTTTAGATCCTGACTCCAGGATGAAGACAACGATGGACCTGATGGAGGGTTTATTCCCTGGAGACGCCAG; TGTGCTGATGGATAGTGGCGCAAAGAGGAAAGCCTTAGACATCACCGCCAGGCGTGCGGGATGTGAAGCC; AAGGCGAGCGACCACAAGGAGGCAGTGAGTGTGCTGGTCAACTGTCCTGCCTACTACAGTGTCTCTGCTG; CCAAGGCGGAGCTGCTCAACAAAATCAAAGACATGCCGGAGGAGCTGCAGGAGGAGGAGGGGCAGGAGGA; TGTCAATGAGAAAAAGGCTGAGCTTATCGGAAGCCTTACCCACAAGCTGGAGAGCCTCCAGGAAGCCAAG; GGGAGCCTGCTCACGGACATCAAGCTGAACAATGCCCTGGGAGAGGAGGTGGAGGCTCTGATCAGTGAGC; TCTGCAAACCCAACGAGTTTGACAAGTACAAGATGTTCATAGGGGACCTGGACAAGGTGGTCAACCTGCT; GCTGTCCCTGTCTGGACGCCTGGCCCGCGTGGAGAACGTCCTCAGAGGCCTTGGTGAAGACGCCAGCAAA; GAGGAAAGGAGTTCTCTGAACGAGAAAAGGAAGGTTCTGGCTGGCCAGCATGAGGATGCACGGGAGCTCA; AGGAGAACCTGGACCGGAGGGAGCGCGTGGTGCTGGATATCCTGGCCAACTACCTGTCCGCCGAGCAGCT; GCAGGACTACCAGCACTTCGTGAAGATGAAGTCCACACTCCTCATTGAGCAGCGGAAGCTGGATGACAAG; ATAAAGCTGGGCCAGGAGCAGGTCAGGTGCCTGCTGGAGTCGCTTCCCTCGGACTTCAGGCCCAAGGCAG; GGGCCATCTCCCTCCCTCCAGCCCTCACCGGCCATGGGACTCCTGGGGGGACATCTGTATTCGGTGGTGT; TTTCCCAACGTTGACCTCTCCTCTTTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001077595
• Insert Size: 5418 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001077595.2, NP_001071063.1
• RefSeq Size: 6838 bp
• RefSeq ORF: 5418 bp
• Locus ID: 27428
• UniProt ID: Q9QXN0
• Cytogenetics: 5 47.29 cM
• Summary: Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2).