Cpvl (NM_001289713) Mouse Untagged Clone

SKU MC227904

Cpvl (untagged) - Mouse carboxypeptidase, vitellogenic-like (Cpvl), transcript variant 2

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Cpvl
• Synonyms: 4933436L16Rik; HVLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227904 representing NM_001289713; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTTCGTGCCAAGTGGAAGATGGTTGTTTCACTGATCTTGTTTATGGTCAGCCCTGGTGATGGACTGT; TTCATGCAGTATACCGAAGTATCCTTGTTTCCCAGTCCTTCAAGGGGGATGCAGGACAGCCTCTTTTTCT; TAGCCCATACATCAAAAACGGGAAGATCAAGGAGGGGCAAAGGAAGAGCATGGTCAGTCCGTTCCCTGGA; ATGAACGACAAGAGTTATGCCGGCTACATCACGGTGAACCAGACTTACAACAGCAACCTCTTCTTCTGGT; TCTTTCCGGCTCGGATGCAGCCTGAGGATGCCCCAGTAGTCCTCTGGCTTCAGGGTGGACCTGGAGGTTC; ATCCATGTTTGGACTCTTTGTAGAACACGGGCCTTATATTATCACAAGTAACATGACCGTTGTGGCCAGA; GACTTCCCTTGGACCTTTACCCTTTCCATGCTCTACATTGATAATCCGGTGGGTACAGGCTTCAGCTTCA; CCGATCATTTCCAGGGATACGCCACCAGCGAGGATGATGTAGCCCAAGACTTGTACAGTGCCCTGATTCA; GTTCTTCACGTTGTTTCCCGAGTATGCAAAGAATGATTTTTACGTCACTGGCGAGTCTTATGCAGGGAAA; TATGTTCCGGCCCTAGCACACTATATCCACTCCCTCAACCCCGTGCGGAAGTTCAAGATTCGCCTAAAAG; GAATTGCCATTGGAGATGCATACACTGATCCTGAATCGATTATAGGAGGGTATGCGGCATTCCTATATGA; AATTGGCTTGTTGGATGAACAGCAGCAGAAGTACTTCCAGAAGCAGTGTAGCAAGTGCGTAAAATACATC; AAAGAGCAGGAGTGGATGAAGGCCTTTGAAATCCTGGATAAGCTGCTAGATGGAGATGTAACAACTGGTT; CATCTTTCTTCCAGAATGTGACAGGATGTACCAATTACTACAACATTTTACAGTGCACGGAACCCAAGGA; GCAAAGTTACTTTGCAAAATTCTTGACGCTCCCCCAAGTGAGACAAGCCATCCACGTGGGAAACCAGAAC; TTCAGTGACGGTGCTGAGGTTGAGAAGCACCTGCGAGAGGACACAGTGAAGTCGGTGAAGCCCTGGCTGT; CTGAGATCATGAATTACTACAAGGTTCTCATCTACAATGGCCAACTGGACATCATCGTGGCGGCTGCCCT; GACAGAGCGCTCCTTGATGGCCATGGACTGGAAGGGATCCCGAGCATACAGAAGGGCACGTCGAAAGGTC; TGGAAGATCTTCAAATCGGATAATGAAGTGGCTGGTTATGTGAGGCGTGTGGGCAAATTCCATCAGGTAA; TCGTCCGAGGTGGAGGACACATTTTGCCCTATGACCAGCCAATGAGATCTTTTGATATGATCAATCGCTT; CATCTATGACAGAGGATGGGAACCTTATAATTCATAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289713
• Insert Size: 1437 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001289713.1, NP_001276642.1
• RefSeq Size: 1622 bp
• RefSeq ORF: 1437 bp
• Locus ID: 71287
• UniProt ID: Q9D3S9
• Cytogenetics: 6 B3
• Summary: This gene encodes a member of the serine carboxypeptidase family of proteases that cleave amino acids from the C-terminus of a protein substrate. The human ortholog of this gene, where it was first characterized, was found to be upregulated during the maturation of monocytes to macrophages. The encoded protein may be involved in antigen processing, digestion of phagocytosed proteins in the lysosome and lamellipodium formation. Disruption of this gene in mice was found to cause embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]; Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1).