Arhgef5 (NM_133674) Mouse Untagged Clone

SKU MC224710

Arhgef5 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 5 (Arhgef5), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Arhgef5
• Synonyms: 2210412D05Rik; AA717842; AW495314; Tim1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224710 representing NM_133674; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGGCTGAGGAGCCTGAATATGGAGTATCTACAGAAGTCCCTGACATAGAAGAATTGAAAACTATCC; CTGAAGGTATCATGAGGAGCAGTCAGATCCCTGCCTTGGATCCTGAAGCTCAAGAAGACCGAGACCCATC; CTATAAGTGGACAGATGGACACAGACCCGTGATGAACCAGTCAAAAGTGTTAAGGGACATGGGTGACCAT; ACACCTAATAGTATGGCAATTTTCTTCAAGAAAGAATCTTCGGATATGGAGACAAGCCAGGAAATCCTTC; TGGCTGAGGCCTGTAACACTCCAGACCAGCAGGAAGCTGTAATCCAGAGCCTTAAAGACAGACTGTCAAG; AACAATAGCTGCCCCTGAGCTCTTGGCATGTGCTGTCCAGGAAGAATGGCTAGACATACCCAGCAAACTA; GACAACAGAGTGGGGGCAGAATTGCAGTCAGAACTTATGTCTTTGACTTTGGCAGTTAGCAAAGAGAAAG; AAGAGGAAGAAACCTCCCCAGACACCTCAATCCCTAGAGGATCTTGGCCTCCCTGCAAAACTCACCCTGG; TGAGACTGAGCAGACCCAGGGCAGTGGAAGTGAACTGCTTAGGCAGGGGAAACAGCTGCAGTTGGAGGCC; ACACAAGAAAATCAAGGGCAAGAAGGCTTCCTTCAATCTCAGGAAGCCCAGGGACTGGAGGAGCAGGAGG; GACAGGAAGTAGAAATTCAGGAGGAAGGAACTCTGAACGAAGGCATTTGTTTTGGTGGGCTCCTGGGAGA; GCAGGAGGAGGTGGAAGAGGGGTTTAACGGCAACGAGGAAGAACAGAAGCAGGGACAGATCCAAAGCTAT; ATGCTACTTGGAGGACAATGGGAAAATGAGGGGCTCAGTGGGGAATTAGAAGGTCTGAATTACAGTGAGA; GAGGCCAAGAGAACAGGGAAAGGAGGGTTTGGGTTCTGAGAGATTCAGAAGAGGAGGGACAGGACCAGGA; ATCCAGGGAGGTGGAAGAGAGAAGGGTAGCCACTCAGTATACAGAGAATCAAAGGTTAGTAGAGAAATCA; GAGATTGTAAAGAGAAAGCAGAGAGATCATGATCAAACAGGAAAAGTGATGCCCGTAAGGGACCAGAAGG; AGGTTGTAGACTCAGGTGACAGGGTCCAAGGGAATGGGGATAGTGGTGGGCAGACAGCAGTGGAAGGGAG; CAGACCAGGAGAGGACAGCAAGCCTTCTCTTCCAGTGGCTTCCGTAGATCCTGAGGTTCTCTCCCCAGGT; ACATTGTTCCCAGGTATTTCTTCCTCTGTGGCTGATATTCCACAGATTCAGAAGGAGCCTGTGTGTGAGG; AACTGAGCCCTCAAGCTCCTGCACTGGAGCCAACAGAATGGTCTCACCAACCCATTTCTCCCCCTGCCTC; TTTTGCTCCTGAAGAATCTCTTGATAATAGGACTCACAACAGCCAGCAAGAAGAATTCAGGCTAAGGAAG; GGGATTGAGGTTGTCTCTGCTAGTACATCTGTGGCTCCTTCAGGGACACGAGATTCACCTCCTTTCAGTC; CTCCTAATGTTTTCTCCAGCACTGCCACCTTGTCACCTGTTAGCTCTTCAGTTATCCTTCCTGAGGAGAC; CCCAACAGCCTCTGCCTCAGCTGACACTCCACATCATTGTGGGCCATGTGAGACTCCTCCACTACCCGCA; AAATCCTCCAGGTACCCATGTGCCACCTCTGACACAGCCAACCCTCACAGCCCTCTTAGCAGCTACACTG; GAGTTACCCAACATCTAAGAAGCAACTCGTTCCCAGGCTCTCACAGGACAGAGCAGACTCCAGACTCTCT; GGGAATGTCACTCTCTTTCTCTCATTTGGAATTACCTCAGAGGCCTCCTAAACCAGCCATCTATGGCTCT; CTGACCCCAAGAAGAAACAGAAGAAGTAGGGATGGTATCGTCTTTTCAGACTCCTCTACTGCTTTGTTTG; CTTTGAAACAGGACTCTGAAGAATTCACTTCAAATCCAGAGAGGCCCAGCAGTCCTCATGGTAGTCCGAC; ATGGGGCTCCCCACAGAACTCAGCCTTTGCCATAGGCTCTCCTGCAAATGTTTCTTCACCACCCACTGTC; TCCATGGATATGACGATACGTGAAGCTTTACTCCCTATTCCCCCAGAGAAGAGACATAGCTACAGTCACA; TAGTGGAGAGGGATGGCCTTCTTCATGAAGTAGCCTCTACACTGAAGCGGCATAGTCATCCTCCTCCACT; GACCCTAAGTTCAGGGCTCCATAGATCTTCTAAAGGCTCATTTTCCCTAGTTCCTGACTCCACTGTGGCA; AGGCAGCACCGCCCTCTGCCGTCTACCCCAGAAAGCCCCAACCATACACAGACCTCCATCCCTTCCAGAC; TGAGATACAACAAACCATTACCCCCAACTCCTGATATGCCTGAGTTCTACCATCCCTCCATCTCTTCCTC; TTATATCTCAAGGATGTATAGGCCTCTACCCCCAGTCCCTATCATAGATCCTTCCTCTGAACCACCTCCA; TTGCCCCCAAAGTCCAGGGGGAGAAGCAAGAGTATCCAGGGAGGAGTTATACATTCAGGAGGTCAAGCCA; AGCCAAGACCTAATAATCAAGACTGGACAGCTTCTACTCTCTCTGTTGGACGAACCTCCTGGCCTCCAGC; CACAGGCAGATCAACAGAATCTTTGCCTCTCACCAGTAGGTGTAATAATGAAGTGTCCCCTGGCCTGGCT; TTCAGCAACATGACAAACCTTCTTAGTCCCTCTTCTCCTACTACTCCTTGGATCCCAGACCTTCAGAGAC; CTACCACTAAGGATGAGTCAGGGCTCACTGAAGAGTCTGAGCCACCAGTGAGAGGATCATTTAGACGATC; TGCCCCTCAGGAGGAATTTAATAATACAAGGAGGTCAGCTTTAGGATCAAGAAAGAACTCAGAAAAACCC; CTCCACCACCAACTGGAGAAGGCATCCAGCTGGCCTCACAGACGCGACCCAGCAAGGACATCAGAAAGTA; GCAGTGAACAGGTTGTTCTGGGCCAGGTACCCAACAAGCAAAAGGGCTGGAACCGGCAAGGCCTTCGTAG; ACCTTCAATTTTGCCTGAAAGCTCTTCAGATTTACGAAATCCAGCTGCGGGAAGACTTCCTGGCTCTTCA; GATTCTGTTGTTTTCCGGGAGAAGAAACCAAAGGAGGGGATGGGAGGCTTTTCAAGACGTTGCTCCAAGC; TCATCTCCTCCCAGCTACTTTATCAGGAGTACAGTGATGTTGTTCTGAACAAGGAGATTCAGAGCCAGCA; GCGGCTGGACAGCCTGGCCGAGCCACATGGGCTCTCTTCCCCACGGCATCGTCGAAAGGCACTGGTCTCT; TCAGACTCCTACCTACAGCGCCTCTCTATGGCCTCCAGTGGCTCCCTCTGGCAGGAAATCCCTGTGGTGC; GCAATAGCACAGTGCTGCTCTCCATGACCCATGAAGATCAAAAACTGCAAGAGGCCAAGTTTGAGCTGAT; TGTGTCAGAAGCATCTTACCTGCGCAGTCTAAACATAGCTGTGGATCATTTCCAACATTCAGCCCAGCTG; CGGGGCACCCTGTCCAACCAGGATCATCAGTGGCTCTTCTCTCGTTTGCAGGATGTACGAGATGTCAGCA; CCACGTTCCTTTCAGACCTAGAAGAAAACTTTGAGAACAACATCTTCTCCTTCCAAGTGTGTGATGTTGT; CTTGAATCATGCCGCAGACTTCCACCGTGTCTACCTGCCTTATGTCACCAACCAGACCTATCAGGAACGC; ACCTTCCAAAGTCTAATGAATAGCAACAGCAGTTTCCGGGAGGTCTTGGAGAAGCTAGAGAGCGACCCCA; TCTGCCAACGCCTGTCTCTCAAGTCCTTTCTGATACTGCCCTTCCAACGCATCACACGTCTCAAGCTGCT; GCTCCAGAACATACTGAAGAGAACTCAGCCTGGCTCCTCAGAAGAGGCAGAGGCCACGAAGGCACACCAT; GCCCTAGAGAAGCTGATTCGAGACTGCAACAGCAATGTGCAGAGAATGCGGCGGACAGAGGAGCTCATCT; ACCTGAGCCAGAAGATTGAGTTTGAGTGCAAAATTTTCCCACTTATTTCACAATCTCGATGGCTGGTGAA; GAGTGGGGAGCTGACGGCCCTTGAGTTCAGTGTCTCCCCAGGGCTGAGAAGGAAACTGACCACTCGTCCA; GTCCACCTACATCTCTTCAATGACTGTCTGTTGCTGTCTCGGCCCCGAGAGGGCAGCCGGTTCCTGGTAT; TTGACCATGCTCCGTTCTCCTCCATCCGAGGGGAGAAGTGTGAAATGAAGCTTCATGGACCTCACAAAAA; CCTCTTCCGTCTCTTTCTCCTGCACAACGCACAGGGCACCCAAGTGGAATTCCTCTTCCGCACTGAGACT; CAAAGTGAAAAGCTTCGCTGGATCTCAGCCTTGGCCATGCCCAGAGAGGAGTTGGACCTCCTTGAGTGTT; ATGACTCCCCACAAGTTCAGTGCCTCCGTGCCTACAAGCCTCGAGAGAATGATGAGTTGGCACTAGAGAA; GGCGGATGTGGTGATGGTCACTCAGCAAAGCAGTGATGGCTGGCTGGAGGGTGTAAGACTCTCAGATGGT; GAGCAAGGCTGGTTTCCTGTCCAGCAGGTAGAGTTCATTTCAAACCCAGAGGTCCGTGCTCAGAACCTCA; AGGAAGCCCATCGAGTCAAGACGGCCAAACTGCAGCTGGTAGAACAGCAAGTCTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133674
• Insert Size: 4746 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_133674.1, NP_598435.1
• RefSeq Size: 5420 bp
• RefSeq ORF: 4746 bp
• Locus ID: 54324
• UniProt ID: E9Q7D5
• Cytogenetics: 6 B2.1
• Summary: Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:19713215, PubMed:21525037). Strongly activates RHOA (PubMed:19713215, PubMed:21525037). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (PubMed:19713215). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:21525037). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (By similarity). Required for SRC-induced podosome formation (PubMed:21525037). Involved in positive regulation of immature dendritic cell migration (PubMed:19713215).[UniProtKB/Swiss-Prot Function]