Nlrp1a (NM_001004142) Mouse Untagged Clone

SKU MC223865

Nlrp1a (untagged) - Mouse NLR family, pyrin domain containing 1A (Nlrp1a), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Nlrp1a
• Synonyms: CARD7; DEFCAP; Gm14; Gm15; NAC; Nalp1; Nalp1a; Nlrp1; PP1044
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223865 representing NM_001004142; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAGAATCTCAGTCCAAGCAGGAAAGTAGCACAAAAGTGGCTCAGCATGAAGGTCAAGAGGATGTGG; ACCCAACCTTCAAAACAAAGAAACTTATGGAGGTGGAGCTAATGAAGCACAGAGTGCAGCTAGAGAGGAA; CTTGAAGCTAAGAACCTTTCCAGGAGCACGTACGAAGCAAGTAAAAGAGGCTCTTTACCCTCTTCTAACA; TGGTCATCAAAAAGCAAGAATCTGTTCCAAAATTTCACAAAACTACTACTCTTTAAAAAATTGTGCCAAA; GAGGCTCAGAAAACTTGGTCAGGGAAAGCTGGTATCCATGTGTACCAGAAGAAGAAGCACATATGATTGA; CATCCAAGATTTATTTGGCCCAAACTTAGGTACTCAGAAAAAGCCTCAATTAGTCATAATAGAAGGAGCT; GCTGGGATTGGGAAGTCAACACTGGCCAGACTTGTGAAGAGAGCCTGGAAGGAAGGCAAGCTTTACAGGA; ATGACTTCCATCATGTCTTCTTCTTCAGCTGCAGAGAGCTGGCCCAGTATGAGCAGCTGAGTCTGGCTGA; GCTCATAGTACAAGGCCAGGAAGTGCCCACAGCTCCCATTAGGCAGATCCTGTCTCACCCTGAGAAGTTG; CTCTTCATTCTGGATGGCATAGATGAGCCAGCATGGGTCTTAGCAGACCAGAATCCTGAGTTATGTCTTC; ACTGGAGTCAAACCCAGCCTGTGCACACTCTGCTGGGCAGTTTACTTGGGAAATCCATCCTTCCTGGGGC; TTCCTTCTTGCTCACAACTCGCACCACAGCTCTACAGAAATTCATTCCTTCCCTGGAGCAGCCATGTCAA; GTGGAAGTCCTAGGATTCACTTTGTTTGAACGGAAAAACTATTTCTACAAATATTTTGGGAAGAAAAAAG; GTGGAGTTACTACTTTTACATTAGTTAAATCAAACTCAGCGCTCCTGACTCTGTGTGAAGTTCCCTGGGT; GTGCTGGTTGGTCTGCACTTGCCTGAAAAAGCAGATGGAGCAGGGTGGAGAGCTCTCACTGACCTCACAG; ACCACCACAGCACTCTGCCTGAAATACCTTTCCCTGACGATCCCAGGGCAGCACATGAGGACCCAGCTCA; GAGACCTCTGCTCACTGGCTGCTGAGGGGGTCTGCCAAAGAAGGACCCTGTTCAGTGAAAGCGATCTCTG; TAAGCAAGGGTTAGATGAGCATGCCATTGCCAGTTTCCTGAAGATTGGTGTCCTTCAAAAGCAGGCCAGC; TCTCTGAGCTACAGCTTTGCCCACTTGTGTCTTCAGGAGTTCTTTGCAGCAATGTCCTACATCTTGGATG; ATAGTGAGGAAAGACATGCTGATATGAAAAATGACAGAATTGTGGAAACACTGGTAGAAAGGTATGGAAG; ACAAAACCTGTTTGAGGCACCCACTGTGCGTTTTTTATTTGGTCTTTTGAGTAAAGAGGAATTGAAAAAA; ATAGAAAAGCTCTTTTCCTGCAGCCTTCATGGAAAGACAAAGTTGAAACTACTATGGCACATCCTAGGGA; AATCCCAACCCCATCAACCACCTTGCCTGGGTTTGCTCCACTGTCTGTATGAAAATCAGGATATGGAGCT; CCTGACACACGTGATGCATGATCTGCAAGGAACAATAGTGCCTGGACCAGATGATTTGGCACACACAGTG; TTGCAGACAAACGTGAAGCACCTGGTGATACAGACAGACATGGACCTCATGGTGGTTACTTTCTGCATTA; AGTTCTGCTGTCACGTGAGGAGTCTTCAGCTGAACAGGAAGGTACAGCAGGGACATAAGTTTACGGCCCC; TGGGATGGTTCTGTACAGATGGACCCCAATCACTGATGCCAGCTGGAAGATTTTCTTCTCCAATCTTAAG; TTAGCCAGAAACCTGGAGGAGTTGGACCTAAGTGGAAATCCACTGAGCTACTATGCAGTACACAGTCTTT; GTACGACCCTGAGAAAGCGTGGCTGCCAACTAAAGACTCTGTGGCTTGTTGAATGCGGCCTCACATCCAC; ATACTGCTCACTTCTGGCCTCAGTACTCAGTGCCCGCTCCAGCCTGACTGAGCTAGACCTGCAGCTGAAT; GACCTGGGTGATGGTGGTGTGAAGATGTTGTGTGAGGGGCTCAGGAATCCTGCCTGCAATCTCAGCATCC; TGTGGCTGGACCAGGCATCTCTCAGTGACCAAGTGATTGCAGAGCTCAGAACTCTGGAGGCAAAGAATCC; AAAGCTGCTCATCTCCAGCACATGGAAGCCACATGTGATGGTCCCTACGATGAACATGGATAAAGAAGAA; GTGGGTGATAGCCAGGCCCTACTGAAGCAGCAGAGACAGCAGTCAGGAGACAAACACATGGAACCTCTGG; GGACTGAAGATGAGTTCTGGGGCCCTACAGGACCTGTGACTACTGAGGTGGTTGACAGAGAGAGGAACCT; GTACCGAGTTCAGTTACCCATGGCTGGTTCCTACCACTGTCCCAGTACAGGACTCCACTTTGTGGTGACA; AGGGCAGTGACAATCGAGATTGAATTCTGTGCCTGGAGCCAATATCTCGACAAGACTCCCTTGCAGCAGA; GTCACATGGTGGTTGGACCTCTGTTTGACATCAAGGCTGAGCAAGGAGCTGTGACTGCAGTGTACCTCCC; TCATTTTGTGGCCCTCCAAGAGGGAATCGTGGACAGCTCCTTGTTCCATGTTGCCCACTTTCAAGAACAT; GGGATGGTCCTAGAAACACCAGCAAGGGTGGAACAGCATTACGCAGTACTGGAAAACCCAAGCTTCTCCC; CAATGGGGATTCTACTGAGAATGATTCCGGCTGTCGGACACTTCATCCCCATCACTTCCACCACACTGAT; CTACTATCACCTCTATCTTGAAGACGTCACCTTTCACCTTTACCTGGTCCCCAATGACTGCAGTATTCGA; AAGGCCATTGATGATGAAGAAATGAAGTTTCAGTTTGTGCGCATAAACAAGCCACCCCCAGTAGACGCTC; TTTATCTTGGTTCCCGCTATATCGTGTCTTCCTCCAAATTGGTGGAAATTATCCCAAAGGAACTAGAGCT; GTGCTACCGAAGCCCTGGGGAATCCCAGCTCTTCTCTGAGATCGACATTGGGCACATGGATTCAGAGATT; AAGCTGCAAATCAAAGACAAGAGGCACATGAATCTCAAATGGGAGGCCTTGCTGAAACCAGGGGATCTCA; GACCTGCACTGCCCAAGATTGCTACAGCCCCCAAAGATGCTCCTTCCTTGCTGCACTTCATGGACCAGCA; TCGGGAGCAGCTGGTGGCCCGAGTGACATCTGTAGATCCTCTCTTGGACAAGCTGCATGGTCTGGTGCTG; AGTGAAGATTCCTATGAAGTAGTGCGGTCTGAGACCACAAACCAAGACAAGATGAGGAAGCTCTTCAGCC; TCAGCCGGTCCTGGAGCTGGGATTGCAAAGACCAATTCTACCAAGCTCTGAAGGAGACCCATCCTCACCT; GGTCATGGATATACTTGAGAAGTTGGGTGGGGTCTCTGTGAAATCCTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004142
• Insert Size: 3549 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001004142.2, NP_001004142.2
• RefSeq Size: 4806 bp
• RefSeq ORF: 3549 bp
• Locus ID: 195046
• UniProt ID: Q2LKU9
• Cytogenetics: 11 43.21 cM
• Summary: As a potential sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of Nlrp1b, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (By similarity) (PubMed:23219391). When activated in the bone marrow, induces the pyroptosis of hematopoietic stem cells and progenitor cells of both myeloid and lymphoid lineages, hence allowing the removal of damaged cells, and the release of IL1B, which induces granulopoiesis (PubMed:23219391). Binds ATP (By similarity).[UniProtKB/Swiss-Prot Function]