Rtn4 (NM_194051) Mouse Untagged Clone

CAT#: MC223370

Rtn4 (untagged) - Mouse reticulon 4 (Rtn4), transcript variant 5, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rtn4
• Synonyms: 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; mKIAA0886; mKIAA4153; NgA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223370 representing NM_194051
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCCCGCTGGCGGGAGGCGGGCAGAAGGGTGGGGCGGCCTCGGAAGCCTGGGTGCCTTCATTGT
  TTGTCGGGGTTTCGGGGAGCACGTGCACCGCTGCCAAGTCGCTGGTTCCCATTCCAGCACGAAGTTCTCG
  CCTATCAGCAGCTAGGAATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACCCTCCTCT
  GCAGAAAAAATTATGGATTTGAAGGAGCAGCCAGGTAACACTGTTTCGTCTGGTCAAGAGGATTTCCCAT
  CTGTCCTGTTTGAAACTGCTGCCTCTCTTCCTTCTCTATCTCCTCTCTCAACTGTTTCTTTTAAAGAACA
  CGGATACCTTGGTAACTTATCAGCAGTGGCATCCACAGAAGGAACTATTGAAGAAACTTTAAATGAAGCT
  TCTAGAGAATTGCCAGAGAGGGCAACAAATCCATTTGTAAATAGAGAGTCAGCAGAGTTTTCAGTATTAG
  AATACTCAGAAATGGGATCATCTTTCAATGGCTCCCCAAAAGGAGAGTCAGCCATGTTAGTAGAAAACAC
  TAAGGAAGAAGTAATTGTGAGGAGTAAAGACAAAGAGGATTTAGTTTGTAGTGCAGCCCTTCATAATCCA
  CAAGAGTCACCTGCGACCCTTACTAAAGTGGTTAAAGAAGACGGAGTTATGTCTCCAGAAAAGACAATGG
  ACATTTTTAATGAAATGAAAATGTCAGTGGTAGCACCTGTGAGGGAAGAGTATGCAGATTTTAAGCCATT
  TGAACAAGCATGGGAAGTGAAAGATACTTATGAGGGAAGTAGGGATGTGCTGGCTGCTAGAGCTAATATG
  GAAAGTAAAGTGGACAAAAAATGCTTTGAAGATAGCCTGGAGCAAAAAGGTCATGGGAAGGATAGTGAAA
  GCAGAAATGAGAATGCTTCTTTCCCCAGGACCCCAGAACTTGTGAAGGACGGCTCCAGAGCGTACATCAC
  CTGTGATTCCTTTAGCTCAGCAACCGAGAGTACTGCAGCAAACATTTTCCCTGTGCTAGAAGATCACACT
  TCAGAAAACAAAACAGATGAAAAAAAAATAGAAGAAAGGAAGGCCCAAATTATAACAGAGAAGACTAGCC
  CCAAAACGTCAAATCCTTTCCTTGTAGCAATACATGATTCTGAGGCAGATTATGTCACAACAGATAATTT
  ATCAAAGGTGACTGAGGCAGTAGTGGCAACCATGCCTGAAGGTCTAACGCCAGATTTAGTTCAGGAAGCA
  TGTGAAAGTGAACTGAACGAAGCCACAGGTACAAAGATTGCTTATGAAACAAAAGTGGACTTGGTCCAGA
  CATCAGAAGCTATACAAGAGTCAATTTACCCCACAGCACAGCTTTGCCCATCATTTGAGGAAGCTGAAGC
  AACTCCGTCACCAGTTTTGCCTGATATTGTTATGGAAGCGCCATTAAATTCTCTCCTTCCAAGCACTGGT
  GCTTCTGTAGCGCAGCCCAGTGCATCCCCACTAGAAGTACCGTCTCCAGTTAGTTATGACGGTATAAAGC
  TTGAGCCTGAAAATCCCCCACCATATGAAGAAGCCATGAGTGTAGCACTAAAAACATCGGACTCAAAGGA
  AGAAATTAAAGAGCCTGAAAGTTTTAATGCAGCTGCTCAGGAAGCAGAAGCTCCTTATATATCCATTGCA
  TGTGATTTAATTAAAGAAACAAAGCTCTCCACTGAGCCAAGTCCAGAGTTCTCTAATTATTCAGAAATAG
  CAAAATTTGAGAAGTCGGTGCCTGATCACTGTGAGCTCGTGGATGATTCCTCACCCGAATCTGAACCAGT
  TGACTTATTTAGTGATGATTCAATTCCTGAAGTCCCACAAACACAAGAGGAGGCTGTGATGCTAATGAAG
  GAGAGTCTCACTGAAGTGTCTGAGACAGTAACACAACACAAACATAAGGAGAGACTTAGTGCTTCACCTC
  AGGAGGTAGGAAAGCCATATTTAGAGTCTTTTCAGCCCAATTTACATATTACAAAAGATGCTGCATCTAA
  TGAAATTCCAACATTGACCAAAAAGGAGACAATTTCTTTGCAAATGGAAGAGTTTAATACTGCAATTTAT
  TCCAATGATGACTTACTTTCTTCTAAGGAAGACAAAATGAAAGAAAGTGAAACATTTTCCGATTCATCTC
  CCATTGAGATAATAGATGAGTTTCCCACATTTGTCAGTGCTAAAGATGATTCTCCTAAGGAGTACACTGA
  CCTAGAAGTATCCAACAAAAGTGAAATTGCTAATGTCCAGAGCGGGGCCAATTCGTTGCCTTGCTCAGAA
  TTGCCCTGTGACCTTTCTTTCAAGAATACATATCCTAAAGATGAAGCACATGTCTCAGATGAATTCTCCA
  AAAGTAGGTCCAGTGTATCTAAGGTGCCCTTATTGCTTCCAAATGTTTCTGCTTTGGAATCTCAAATAGA
  AATGGGCAACATAGTTAAACCCAAAGTACTTACGAAAGAAGCAGAGGAAAAACTTCCTTCTGATACAGAG
  AAAGAGGACAGATCCCTGACAGCTGTATTGTCAGCAGAGCTGAATAAAACTTCAGTTGTTGACCTCCTGT
  ACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCTTATTCCTGCTGCTGTCTCTGACAGT
  GTTCAGCATTGTCAGTGTAACGGCCTACATTGCCTTGGCCCTGCTCTCTGTGACTATCAGCTTTAGGATA
  TATAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATTTGGAATCTG
  AAGTTGCCATATCAGAGGAATTGGTTCAGAAATATAGTAATTCTGCTCTTGGTCATGTGAACAGCACAAT
  AAAAGAATTGAGGCGTCTCTTCTTAGTTGATGATTTAGTTGATTCCCTGAAGTTTGCAGTGTTGATGTGG
  GTATTTACTTACGTTGGTGCCTTGTTCAATGGTTTGACACTACTGATTTTAGCTCTGATCTCACTCTTCA
  GTATTCCTGTTATATATGAACGGCATCAGGCGCAGATAGATCATTATCTAGGACTTGCAAACAAGAGCGT
  TAAGGATGCCATGGCCAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCAGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_194051
• Insert Size: 3141 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194051.3, NP_918940.1
• RefSeq Size: 5558 bp
• RefSeq ORF: 3141 bp
• Locus ID: 68585
• UniProt ID: Q99P72
• Cytogenetics: 11 A3.3
• Gene Summary: Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) uses a different segment for its 5' end, compared to variant 1. The resulting protein (isoform D) has a shorter and distinct N-terminus when it is compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.