Rtn4 (NM_194051) Mouse Tagged ORF Clone

CAT#: MR226113

  • TrueORF®

Rtn4 (Myc-DDK-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 5

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_194051" in other vectors (4)


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Reconstitution Protocol

USD 1,002.00

5 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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Other products for "Rtn4"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Rtn4
Synonyms 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; mKIAA0886; mKIAA4153; NgA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226113 representing NM_194051
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCCCCGCTGGCGGGAGGCGGGCAGAAGGGTGGGGCGGCCTCGGAAGCCTGGGTGCCTTCATTGT
TTGTCGGGGTTTCGGGGAGCACGTGCACCGCTGCCAAGTCGCTGGTTCCCATTCCAGCACGAAGTTCTCG
CCTATCAGCAGCTAGGAATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACCCTCCTCT
GCAGAAAAAATTATGGATTTGAAGGAGCAGCCAGGTAACACTGTTTCGTCTGGTCAAGAGGATTTCCCAT
CTGTCCTGTTTGAAACTGCTGCCTCTCTTCCTTCTCTATCTCCTCTCTCAACTGTTTCTTTTAAAGAACA
CGGATACCTTGGTAACTTATCAGCAGTGGCATCCACAGAAGGAACTATTGAAGAAACTTTAAATGAAGCT
TCTAGAGAATTGCCAGAGAGGGCAACAAATCCATTTGTAAATAGAGAGTCAGCAGAGTTTTCAGTATTAG
AATACTCAGAAATGGGATCATCTTTCAATGGCTCCCCAAAAGGAGAGTCAGCCATGTTAGTAGAAAACAC
TAAGGAAGAAGTAATTGTGAGGAGTAAAGACAAAGAGGATTTAGTTTGTAGTGCAGCCCTTCATAATCCA
CAAGAGTCACCTGCGACCCTTACTAAAGTGGTTAAAGAAGACGGAGTTATGTCTCCAGAAAAGACAATGG
ACATTTTTAATGAAATGAAAATGTCAGTGGTAGCACCTGTGAGGGAAGAGTATGCAGATTTTAAGCCATT
TGAACAAGCATGGGAAGTGAAAGATACTTATGAGGGAAGTAGGGATGTGCTGGCTGCTAGAGCTAATATG
GAAAGTAAAGTGGACAAAAAATGCTTTGAAGATAGCCTGGAGCAAAAAGGTCATGGGAAGGATAGTGAAA
GCAGAAATGAGAATGCTTCTTTCCCCAGGACCCCAGAACTTGTGAAGGACGGCTCCAGAGCGTACATCAC
CTGTGATTCCTTTAGCTCAGCAACCGAGAGTACTGCAGCAAACATTTTCCCTGTGCTAGAAGATCACACT
TCAGAAAACAAAACAGATGAAAAAAAAATAGAAGAAAGGAAGGCCCAAATTATAACAGAGAAGACTAGCC
CCAAAACGTCAAATCCTTTCCTTGTAGCAATACATGATTCTGAGGCAGATTATGTCACAACAGATAATTT
ATCAAAGGTGACTGAGGCAGTAGTGGCAACCATGCCTGAAGGTCTAACGCCAGATTTAGTTCAGGAAGCA
TGTGAAAGTGAACTGAACGAAGCCACAGGTACAAAGATTGCTTATGAAACAAAAGTGGACTTGGTCCAGA
CATCAGAAGCTATACAAGAGTCAATTTACCCCACAGCACAGCTTTGCCCATCATTTGAGGAAGCTGAAGC
AACTCCGTCACCAGTTTTGCCTGATATTGTTATGGAAGCGCCATTAAATTCTCTCCTTCCAAGCACTGGT
GCTTCTGTAGCGCAGCCCAGTGCATCCCCACTAGAAGTACCGTCTCCAGTTAGTTATGACGGTATAAAGC
TTGAGCCTGAAAATCCCCCACCATATGAAGAAGCCATGAGTGTAGCACTAAAAACATCGGACTCAAAGGA
AGAAATTAAAGAGCCTGAAAGTTTTAATGCAGCTGCTCAGGAAGCAGAAGCTCCTTATATATCCATTGCA
TGTGATTTAATTAAAGAAACAAAGCTCTCCACTGAGCCAAGTCCAGAGTTCTCTAATTATTCAGAAATAG
CAAAATTTGAGAAGTCGGTGCCTGATCACTGTGAGCTCGTGGATGATTCCTCACCCGAATCTGAACCAGT
TGACTTATTTAGTGATGATTCAATTCCTGAAGTCCCACAAACACAAGAGGAGGCTGTGATGCTAATGAAG
GAGAGTCTCACTGAAGTGTCTGAGACAGTAACACAACACAAACATAAGGAGAGACTTAGTGCTTCACCTC
AGGAGGTAGGAAAGCCATATTTAGAGTCTTTTCAGCCCAATTTACATATTACAAAAGATGCTGCATCTAA
TGAAATTCCAACATTGACCAAAAAGGAGACAATTTCTTTGCAAATGGAAGAGTTTAATACTGCAATTTAT
TCCAATGATGACTTACTTTCTTCTAAGGAAGACAAAATGAAAGAAAGTGAAACATTTTCCGATTCATCTC
CCATTGAGATAATAGATGAGTTTCCCACATTTGTCAGTGCTAAAGATGATTCTCCTAAGGAGTACACTGA
CCTAGAAGTATCCAACAAAAGTGAAATTGCTAATGTCCAGAGCGGGGCCAATTCGTTGCCTTGCTCAGAA
TTGCCCTGTGACCTTTCTTTCAAGAATACATATCCTAAAGATGAAGCACATGTCTCAGATGAATTCTCCA
AAAGTAGGTCCAGTGTATCTAAGGTGCCCTTATTGCTTCCAAATGTTTCTGCTTTGGAATCTCAAATAGA
AATGGGCAACATAGTTAAACCCAAAGTACTTACGAAAGAAGCAGAGGAAAAACTTCCTTCTGATACAGAG
AAAGAGGACAGATCCCTGACAGCTGTATTGTCAGCAGAGCTGAATAAAACTTCAGTTGTTGACCTCCTGT
ACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCTTATTCCTGCTGCTGTCTCTGACAGT
GTTCAGCATTGTCAGTGTAACGGCCTACATTGCCTTGGCCCTGCTCTCTGTGACTATCAGCTTTAGGATA
TATAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATTTGGAATCTG
AAGTTGCCATATCAGAGGAATTGGTTCAGAAATATAGTAATTCTGCTCTTGGTCATGTGAACAGCACAAT
AAAAGAATTGAGGCGTCTCTTCTTAGTTGATGATTTAGTTGATTCCCTGAAGTTTGCAGTGTTGATGTGG
GTATTTACTTACGTTGGTGCCTTGTTCAATGGTTTGACACTACTGATTTTAGCTCTGATCTCACTCTTCA
GTATTCCTGTTATATATGAACGGCATCAGGCGCAGATAGATCATTATCTAGGACTTGCAAACAAGAGCGT
TAAGGATGCCATGGCCAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR226113 representing NM_194051
Red=Cloning site Green=Tags(s)

MAPPLAGGGQKGGAASEAWVPSLFVGVSGSTCTAAKSLVPIPARSSRLSAARNETLFALPAASEPVIPSS
AEKIMDLKEQPGNTVSSGQEDFPSVLFETAASLPSLSPLSTVSFKEHGYLGNLSAVASTEGTIEETLNEA
SRELPERATNPFVNRESAEFSVLEYSEMGSSFNGSPKGESAMLVENTKEEVIVRSKDKEDLVCSAALHNP
QESPATLTKVVKEDGVMSPEKTMDIFNEMKMSVVAPVREEYADFKPFEQAWEVKDTYEGSRDVLAARANM
ESKVDKKCFEDSLEQKGHGKDSESRNENASFPRTPELVKDGSRAYITCDSFSSATESTAANIFPVLEDHT
SENKTDEKKIEERKAQIITEKTSPKTSNPFLVAIHDSEADYVTTDNLSKVTEAVVATMPEGLTPDLVQEA
CESELNEATGTKIAYETKVDLVQTSEAIQESIYPTAQLCPSFEEAEATPSPVLPDIVMEAPLNSLLPSTG
ASVAQPSASPLEVPSPVSYDGIKLEPENPPPYEEAMSVALKTSDSKEEIKEPESFNAAAQEAEAPYISIA
CDLIKETKLSTEPSPEFSNYSEIAKFEKSVPDHCELVDDSSPESEPVDLFSDDSIPEVPQTQEEAVMLMK
ESLTEVSETVTQHKHKERLSASPQEVGKPYLESFQPNLHITKDAASNEIPTLTKKETISLQMEEFNTAIY
SNDDLLSSKEDKMKESETFSDSSPIEIIDEFPTFVSAKDDSPKEYTDLEVSNKSEIANVQSGANSLPCSE
LPCDLSFKNTYPKDEAHVSDEFSKSRSSVSKVPLLLPNVSALESQIEMGNIVKPKVLTKEAEEKLPSDTE
KEDRSLTAVLSAELNKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRI
YKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMW
VFTYVGALFNGLTLLILALISLFSIPVIYERHQAQIDHYLGLANKSVKDAMAKIQAKIPGLKRKAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_194051
ORF Size 3138 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_194051.3, NP_918940.1
RefSeq Size 5558 bp
RefSeq ORF 3141 bp
Locus ID 68585
UniProt ID Q99P72
Cytogenetics 11 A3.3
MW 114.7 kDa
Gene Summary Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities.[UniProtKB/Swiss-Prot Function]

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