Hltf (NM_144959) Mouse Untagged Clone

SKU MC222000

Hltf (untagged) - Mouse helicase-like transcription factor (Hltf), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Hltf
• Synonyms: AF010600; BC057116; P113; Smarca3; Snf2l3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222000 representing NM_144959; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCCTATACGTTCACGAGGGGTCCTGTTTGGAAGTATTCGCAGTCTGTCCAGTATGGATCACACGAAA; ATATTCCAAGACTTTCATACTCAACTTTCTTGCCACATTTTGAATTCCAAGACATTATTCCTCCAGATGA; CTTCCTAACGAGTGATGAAGAGCAAGATTTGGTTTTATTTGGAACTATGAGAGGACAAGTTGTTGGACTA; CGCTATTACACAGGAGTTGTAAATAATAATGAAATGGTTGCTTTACAACGAGAGCCTAATAATCCCTATG; ATAAGAATGCTATTAAAGTAAATAATGTGAATGGAAATCAGGTTGGCCATATAAAGAGAGAGATTGCAGC; TGCCGTGGCCTATATCATGGACAACAAATTGGCACAAGTGGAAGGTGTAGTTCCTTTTGGTGCAAGTAAT; ACTTTTACCATGCCTCTTTATATGACTTTCTGGGGAAAAGAAGAAAATAGAAACGTGGTTTTAGAGCAGT; TGAAGAAACATGGATTTAAGTTGGGTCCCACACCAAAGACTTTAGGGTCGAGTTTGGAGAACGCATGGGG; CTCTGGAAGAGCTGGACCAAGCTATAGTAGGCCGGCTCATGTGGCAGTACAGATGACAACTGACCAGCTC; AAAACGGAATTTGACAAATTATTTGAAGATTTAAAAGAAGATGATAGAACTGTTGAAATGGAACCAGCTG; AGGCTATTGAAACACCGTTGCTTCCACACCAAAAACAAGCTCTAGCTTGGATGATCGCACGAGAGAACAG; TAAAGAACTACCACCATTCTGGGAACAGCGCAATGACTTATATTATAATACAATAACAAATTTTTCTGTG; AAGGAACGACCGGAAAATGTTCATGGAGGAATTTTAGCGGATGATATGGGTTTGGGCAAAACTCTTACTG; CTATTGCTGTCATTCTTACCAACTTTGATGATGGCAGGCCTCTTCTCAGTAAGAGAGGCAAGAAGAATCA; TCCAGGAAAGGAATACAAGGATGAGACCATTAAACGCAGAGGAAGTAATATGGACAAGAAGGAAGATGGA; CACTCAGAGTCATCCACGTGTGGTGAAGAACCCAGTATTTCAGGAACTCCGGAGAAGAGTAGCTGTACTC; TGTCGCAGCTGTCTAGCGTCTGCCCTAAGAGAAGAAAAATTTCTGTACAGTACATTGAAAGTAGTGATTC; AGAGGAAATCGAGACAAGCGAACTGCCCCAGAAAATGAAAGGCAAGCTGAAAAATGTACAGTTAAATACT; AAAAGTAGAGTAAAAGGATCATCTAAGGTTAAGGAAGATTCAAAATTTGCACTTACTTTTTTTGCCTCTG; CAACACAAAGGAAAATGTTGAAAAAAGGCATGTCTATGATGGAGTGCTCAGAGGCATGTGACACTGGAGA; GAGGACAAGAGCAACACTGATCATCTGTCCACTCTCTGTGCTCAGCAACTGGATTGACCAGTTTGGACAA; CATGTAAAATCAGAAGTGCACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGATTCAGCCT; GGCTTTCAAAACAGGATATTATTTTGACTACTTACAATATTTTAACTCATGATTATGGAACTAAAGATGA; TAGTCCATTGCATAGCATCAAGTGGTTAAGGGTGATCCTGGATGAAGGCCATGCCATTCGAAATCCAAAC; GCTCAGCAGACAAAAGCTGTACTTGAGTTAGAGGCAGAAAGAAGATGGGTATTGACAGGTACTCCTATCC; AGAATTCTTTAAAGGACTTGTGGTCTCTTCTGTCCTTCTTAAAACTTAAGCCATTTATTGATAGAGAGTG; GTGGTATAGGATCATACAGCGTCCTGTCACTACAGGAGATGAGGGAGGACTTAGGCGTTTACAGTCCTTA; ATAAAAAATATCACGCTTAGAAGAACAAAGACAAGCAAAATTAAAGGGAAACCTGTTTTGGAGTTGCCAG; AAAGGAAAGTATTTATTCAGCACATTACTCTGTCAGAAGAAGAGAGAAAGATTTATCAGTCTGTTAAAAA; TGAAGGCAAAGCTGCCATTGGAAGGTATTTTACTGAAGGGACTGTCCTTGCACACTATGCAGATGTCTTG; GGTCTTCTGCTTAGATTGCGGCAGATTTGTTGCCATACCCATCTTCTTACAAACGGAATGTCTTCAAGTG; GCCCCTCCAGAAGTGACACGCCTGAAGAGCTGAGAAAGATGTTAATAGAGAAGATGAAGATAATTCTGAG; CTCTGGTTCAGATGAGGAATGTGCAATCTGCCTGGATTCCTTAACATTTCCTGTGATAACCCACTGTGCA; CACGTGTTCTGTAAACCTTGTATCTGTCAGGTCATTCACAGTGAGCAGCCACATGCAAAGTGTCCTTTGT; GCAGAAATGAGATACATGGAGATAATCTATTAGAATGTCCTCCCGAAGAATTGGCATGTGACAGTGACAA; AGAGTCCAGTATGGAATGGAAGTCCAGCTCAAAGGTAAAGTCCACATGCGTAAATATTCTAATGTTTCAT; ACTTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144959
• Insert Size: 2526 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144959.2, NP_659208.2
• RefSeq Size: 3059 bp
• RefSeq ORF: 2526 bp
• Locus ID: 20585
• Cytogenetics: 3 6.15 cM
• Summary: Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity. This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA (By similarity).[UniProtKB/Swiss-Prot Function]