G2e3 (NM_001167964) Mouse Untagged Clone

SKU MC219864

G2e3 (untagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3), transcript variant 3, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: G2e3
• Synonyms: 6030408C04Rik; 9030416F18; AW046379; D930034K21Rik; mKIAA1333
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219864 representing NM_001167964; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATGAAAATAAACCTGATAATTCACAAAGTCTTGCTTGTGTTTTCTGCCGAAAAAATGATGACTGTC; CCAATAAATACGGAGAAAAGAAAACATATGAAAAATGGAATTTTAGTGTCCACTACTACTGCTTGTTAAT; GTCAAGTGGAATTTGGCAGAGAGGTAAAGAAGAAGAAGGAGTTTATGGCTTCCTAATAGAAGACATCAGG; AAGGAAGTCCAGAGAGCCTCCAAACTGAAGTGTACAGTTTGCAAGAAGAATGGTGCTTCAATTGGATGTG; TGGTACCCACGTGTAAACGAAGTTATCACTTGCCTTGTGGACTTCAGAAAGAATGTATTTTCCAGTTCAC; TGACAATTTTGCGTCATTTTGCTGGAAACATCGACCCGTTCAAGCAATTACATCTAATAAATATAGTAGT; TCTTTACCATGTACTATCTGTTTGGAATTTGTCGAGCCTATTCCTACTTATAACATACTACAAAGTCCCT; GTTGTAAGAATGCTTGGTTTCATAGAGACTGTTTACAGGTCCAAGCAATAAATGCAGGAGTGTTTTTCTT; TAGATGTACACTATGCAATAATACAGACATCTTTCAGAAGGAGATGTTGAGAATGGGAATTCATATTCCT; GAAAAAGATGCATCCTGGGAATTAGAAGAAAACGCGTATCAAGAGCTGCTGCAGAGCCACGACCGCTGTG; ATATTCGAAGATGTCACTGCAAAAAAGGCCGAGACTACAACGAACCTAACAGCAAATGGGAAGTAAAGCG; GTGTCAGAGTTGTGGTTCTAGTGGAACACACTTAGCCTGTTCCTCACTACAGTCTTGGGAACAAAATTGG; GAGTGTTTGGACTGTAGAAGAATTACCTACACTTCAGATTTCCAAAAGGCCCCCAAACACCCATTAGCCA; ACTCTACTAATGTGACAGTTACTGACTGCTTGTTGGAAGAGTCATCATCTAAGCTCCCCAGACAGTCAAC; TGTAGCCCAGCATAAAGAACTACTGAGTTTATTTTTATCTGTATTTACAGCTGTGAAAGAGAATCTTTAC; TATGAAGTTGGCAAAATGCTTGCCATTTCTTTAGTTCATGGTGGTCCTTCGCCTGGTTTCTTTTCTGAAA; CCTTGTTTAACTGCCTTGCTTATGGACCAGAAAATACTCTACCAACTTTGGATGATGTATCAGACATTGA; TGTTGCACAGATTATAATCAAGATAGATTCTGCAACTGATTTAAACATTTTAAATTCAGTAATAAGCCAA; CACTATAACTACTTAGAGGTCAGTGGATGTTTAAGACTTACGACATCATTAAGTGATAAATTCATGTTGG; TAAAAGACATCCTTTTCTACCATGTAATTAATAGAGTCAAGGCACCTTTTGAGAGTTTTAAACAGGGTCT; GAAAACGCTTGGTGTTTTAGAGAAAATTCAAACTTACCCAGAAGCATTTTATAAGATCCTATGTCATAAA; CCTGAGAATCTCTCTGCAAAAAACCTCAGTGACCTCTTCACAATTCACTCGGTAGCGGACGTACAGACTT; TGAGGTTTTGGAACAGTTACTTAAAGGCTATAGAGGATGGTAAATCTGCAACAACGATGGAAGATATTCT; TATTTTTGCAACTGGCTGTAGTTCTGTCCCACCAACTGGATTTAAACCCTCTCTGTCAGTTGAATGTCTA; CATGTGGATTTTCCTGTTGCAGACAAATATAGAAACCATTTAGTTCTACCAGCCACCAACACGTATGAAG; AGTTCCAAGAAAATATGGATTTCACCATAAGAGACACTCTAAGATTAGAAAAGGAAGAAAGGTCTCACAT; ACTACCTCGGACATTAAATGTTTCTTCTAATGAAGAAATGCTTATTTAA; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001167964
• Insert Size: 1869 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001167964.1, NP_001161436.1
• RefSeq Size: 6352 bp
• RefSeq ORF: 1869 bp
• Locus ID: 217558
• UniProt ID: Q5RJY2
• Cytogenetics: 12 B3
• Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Required for prevention of apoptotic death in early embryogenesis.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (3) is missing an internal coding exon and uses an alternate acceptor splice site at another coding exon compared to variant 1. However, it retains the reading frame and encodes a shorter isoform (3) lacking an internal protein segment compared to isoform 1.