Mpl (NM_010823) Mouse Untagged Clone

SKU MC219790

Mpl (untagged) - Mouse myeloproliferative leukemia virus oncogene (Mpl), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Mpl
• Synonyms: c-mpl; CD110; hlb219; TPO-R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219790 representing NM_010823; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCCTCTTGGGCCCTCTTCATGGTCACCTCCTGCCTCCTCTTGGCCCTTCCAAACCAGGCACAAGTCA; CCAGCCAAGATGTCTTCTTGCTGGCCTTGGGCACAGAGCCCCTGAACTGCTTCTCCCAAACATTTGAGGA; CCTCACCTGCTTCTGGGATGAGGAAGAGGCAGCACCCAGTGGGACATACCAGCTGCTGTATGCCTACCGA; GGAGAGAAGCCCCGTGCATGCCCCCTGTATTCCCAGAGTGTGCCCACCTTTGGAACCCGGTATGTGTGCC; AGTTTCCAGCCCAGGATGAAGTGCGCCTCTTCTTTCCGCTGCACCTCTGGGTGAAGAATGTGTCCCTCAA; CCAGACTTTGATCCAGCGGGTGCTGTTTGTGGATAGTGTGGGCCTGCCAGCTCCCCCCAGGGTCATCAAG; GCCAGGGGTGGGAGCCAACCAGGGGAACTTCAGATCCACTGGGAGGCCCCTGCTCCTGAAATCAGTGACT; TCCTGAGGCATGAACTCCGCTATGGCCCCACGGACTCCAGCAACGCCACTGCCCCCTCCGTCATTCAGCT; GCTCTCCACAGAAACCTGCTGCCCCACTTTGTGGATGCCGAACCCAGTCCCTGTTCTTGACCAGCCTCCG; TGTGTTCATCCGACAGCATCCCAACCGCATGGACCAGCTCCATTTCTGACAGTGAAGGGTGGAAGCTGTC; TCGTCTCAGGCCTCCAGGCTGGCAAATCCTACTGGCTCCAGCTACGCAGCCAACCCGACGGGGTCTCCCT; TCGTGGCTCCTGGGGACCCTGGTCCTTCCCTGTGACTGTGGATCTTCCAGGAGATGCAGTGACAATTGGA; CTTCAGTGCTTTACCTTGGATCTGAAGATGGTCACCTGCCAGTGGCAGCAACAAGACCGCACTAGCTCCC; AAGGCTTCTTCCGCCACAGCAGGACGAGGTGCTGCCCCACAGACAGGGACCCCACCTGGGAGAAATGTGA; AGAGGAGGAACCGCGTCCAGGATCACAGCCCGCTCTCGTCTCCCGCTGCCACTTCAAGTCACGAAATGAC; AGTGTTATTCACATCCTTGTAGAGGTGACCACAGCGCAAGGTGCCGTTCACAGCTACCTGGGCTCCCCTT; TTTGGATCCACCAGGCTGTGCTCCTTCCCACCCCGAGCCTGCACTGGAGGGAGGTCTCAAGTGGAAGGCT; GGAGTTGGAGTGGCAGCACCAGTCATCTTGGGCAGCTCAAGAGACCTGCTACCAGCTCCGGTACACGGGA; GAAGGCCGTGAGGACTGGAAGGTGCTGGAGCCATCTCTCGGTGCCCGGGGAGGGACCCTAGAGCTGCGCC; CCCGAGCTCGCTACAGCTTGCAGCTGCGTGCCAGGCTCAACGGCCCCACCTACCAAGGTCCCTGGAGCGC; CTGGTCTCCCCCAGCTAGGGTGTCCACGGGCTCCGAGACTGCTTGGATCACCTTGGTGACTGCTCTGCTC; CTGGTGCTGAGCCTCAGTGCCCTTCTGGGCCTACTGCTGCTAAAGTGGCAATTTCCTGCGCACTACAGGA; GACTGAGGCATGCTTTGTGGCCCTCGCTTCCAGACCTACACCGGGTCCTAGGCCAGTACCTCAGAGACAC; TGCAGCCCTAAGTCCTTCTAAGGCCACGGTTACCGATAGCTGTGAAGAAGTGGAACCCAGCCTCCTGGAA; ATCCTCCCTAAGTCCTCAGAGAGCACTCCTTTACCTCTGTGTCCCTCCCAACCTCAGATGGACTACAGAG; GACTGCAACCTTGCCTGCGGACCATGCCCCTGTCTGTGTGTCCACCCATGGCTGAGACGGGGTCCTGCTG; CACCACACACATTGCCAACCACTCCTACCTACCACTAAGCTATTGGCAGCAGCCCTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010823
• Insert Size: 1878 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_010823.3, NP_034953.2
• RefSeq Size: 2934 bp
• RefSeq ORF: 1878 bp
• Locus ID: 17480
• UniProt ID: Q08351
• Cytogenetics: 4 54.61 cM
• Summary: Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.