Arih1 (NM_019927) Mouse Untagged Clone

CAT#: MC218921

Arih1 (untagged) - Mouse ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arih1
• Synonyms: Ari; Ari1; AU021774; Hari; Hhari; Ubch7bp; Uip77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218921 representing NM_019927
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGGACGAGGGCTACAACTACGAGTTCGACGAGGACGAGGAGTGCAGCGAGGAGGACAGCGGCG
  CCGAGGAGGAGGAGGACGACGACGAGGACGAGCCGGACGATGATAACCTGGATCTGGGCGAGGTGGAGCT
  GGTGGAGCCCGGGCTGGGCGTCGGCGGGGAGCGAGACGGACTGCTGTGCGGGGAGACTGGCGGTGGCGGC
  GGCAGCGCTCTGGGTCCGGGAGGCGGCGGCGGCGGTGGCGGTGGCGGCGGCGGCCCGGGACACGAGCAGG
  AGGAGGATTACCGCTACGAGGTGCTCACGGCCGAACAGATCCTGCAGCACATGGTGGAATGTATCCGGGA
  GGTCAACGAGGTCATCCAGAATCCAGCAACTATCACGAGAATACTCCTTAGCCACTTCAATTGGGATAAA
  GAGAAGCTAATGGAAAGGTACTTTGATGGAAACCTGGAGAAGCTCTTTGCTGAGTGTCATGTAATTAATC
  CAAGTAAAAAGTCTCGAACTCGCCAGATGAATACAAGGTCATCGGCACAGGATATGCCGTGTCAGATCTG
  CTACTTGAACTACCCTAACTCGTATTTTACTGGCCTTGAATGTGGACATAAGTTTTGTATGCAGTGCTGG
  AGTGAATATTTAACTACAAAAATAATGGAGGAAGGCATGGGGCAGACTATTTCGTGTCCTGCGCATGGTT
  GTGATATCTTAGTGGATGACAACACAGTTATGCGCCTGATCACAGATTCCAAAGTTAAGTTAAAATATCA
  ACATTTAATAACAAATAGCTTTGTAGAGTGCAACCGACTGTTAAAGTGGTGTCCTGCCCCAGATTGCCAC
  CATGTTGTTAAAGTCCAGTATCCTGATGCAAAACCAGTTCGCTGCAAATGTGGCCGCCAGTTTTGCTTTA
  ACTGTGGTGAAAATTGGCATGATCCAGTTAAATGTAAGTGGTTGAAGAAGTGGATTAAAAAGTGTGATGA
  TGACAGTGAAACTTCTAATTGGATTGCAGCTAACACAAAGGAATGTCCCAAATGCCATGTCACAATTGAG
  AAGGATGGCGGTTGTAATCACATGGTCTGTCGTAACCAGAACTGTAAAGCAGAATTTTGCTGGGTGTGTC
  TTGGCCCTTGGGAACCGCATGGATCTGCCTGGTACAACTGTAACCGCTATAATGAGGATGATGCAAAGGC
  AGCAAGAGATGCCCAAGAGCGATCTAGGGCGGCCCTGCAGAGATACCTGTTCTACTGTAATCGCTATATG
  AACCACATGCAGAGTCTACGCTTCGAGCATAAGCTGTATGCTCAGGTGAAACAGAAGATGGAGGAGATGC
  AGCAGCACAACATGTCCTGGATCGAGGTGCAGTTCCTGAAGAAAGCAGTTGATGTCCTCTGCCAGTGTCG
  TGCCACACTCATGTACACTTATGTCTTCGCTTTCTACCTCAAAAAGAATAACCAGTCCATTATCTTTGAG
  AATAATCAAGCAGATCTAGAAAATGCCACAGAGGTGCTTTCGGGCTACCTTGAACGAGATATTTCCCAAG
  ATTCTCTGCAAGATATAAAGCAGAAAGTCCAAGATAAGTACAGATACTGTGAGAGTCGACGAAGGGTTTT
  GTTACAGCATGTGCATGAAGGCTATGAAAAAGATCTGTGGGAGTACATTGAGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019927
• Insert Size: 1668 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019927.2, NP_064311.2
• RefSeq Size: 6435 bp
• RefSeq ORF: 1668 bp
• Locus ID: 23806
• UniProt ID: Q9Z1K5
• Cytogenetics: 9 B
• Gene Summary: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2.[UniProtKB/Swiss-Prot Function]