Kpna6 (NM_008468) Mouse Untagged Clone

SKU MC217568

Kpna6 (untagged) - Mouse karyopherin (importin) alpha 6 (Kpna6), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Kpna6
• Synonyms: IPOA7; Kpna5; NPI-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217568 representing NM_008468; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGACCATGGCAAGCCCAGGGAAGGACAATTATCGAATGAAGAGCTATAAGAACAATGCCTTAAACC; CTGAGGAAATGAGACGAAGGAGGGAGGAAGAGGGCATTCAGCTTCGGAAACAGAAGCGGGAACAACAACT; TTTTAAACGGAGAAATGTAGAGCTGATTAATGAAGAAGCTGCCATGTTTGATAGTCTGCTGATGGATTCC; TACGTGAGCTCTACAACTGGGGAGAGCGTGATCACGAGAGAGATGGTAGAGATGCTCTTCTCGGACGACT; CTGACTTGCAGTTAGCAACCACGCAGAAATTCCGGAAACTCCTGTCCAAAGAGCCGAGTCCTCCAATAGA; TGAAGTTATTAACACTCCAGGAGTAGTTGATCGGTTTGTGGAGTTTCTGAAGAGAAATGAGAATTGTACA; TTGCAGTTTGAAGCTGCCTGGGCTCTAACAAACATTGCCTCTGGAACGTCTCAGCAGACCAAAATTGTCA; TTGAAGCAGGAGCTGTCCCCATTTTTATAGAGTTACTTAACTCAGACTTTGAAGATGTACAAGAACAGGC; AGTGTGGGCGCTTGGAAACATCGCTGGAGATAGCTCGCTTTGCAGGGATTATGTCTTGAATTGCTCCATC; CTTAACCCCTTGTTAACACTCCTTACTAAGTCCACACGACTGACAATGACACGGAATGCAGTCTGGGCCC; TGTCAAACCTCTGCCGAGGCAAGAACCCTCCTCCAGAGTTTGCAAAGGTCTCTCCTTGTTTGCCTGTATT; GTCCCGACTGCTCTTCAGCAGTGACTCAGACTTGCTGGCAGATGCTTGCTGGGCCCTCTCCTACCTGTCT; GATGGCCCCAATGAGAAGATCCAGGCAGTCATAGACTCTGGAGTCTGCCGGAGATTGGTAGAGCTTCTGA; TGCACAACGATTACAAAGTGGCGTCTCCTGCCTTGAGAGCTGTGGGTAATATTGTCACTGGGGATGACAT; CCAGACCCAGGTCATTCTTAATTGTTCAGCCCTCCCTTGCCTCCTCCACCTACTGAGCAGCTCTAAGGAA; TCAATCCGGAAGGAAGCTTGCTGGACCATTTCAAACATCACTGCTGGCAACAGGGCTCAAATACAGGCTG; TCATAGATGCAAACATCTTCCCTGTATTGATTGAAATCCTTCAGAAAGCAGAGTTCCGTACAAGGAAAGA; AGCAGCCTGGGCCATCACCAACGCCACATCAGGAGGAACTCCTGAGCAAATCAGGTACCTGGTATCACTG; GGCTGCATTAAGCCCCTGTGTGACTTGCTGACTGTAATGGATTCAAAAATTGTGCAAGTGGCCCTCAATG; GACTAGAGAACATTCTTCGGCTTGGAGAGCAAGAAAGCAAGCGTAGTGGCTCAGGGGTCAATCCCTACTG; TGGCCTCATCGAGGAAGCCTATGGCTTGGATAAAATTGAGTTTCTCCAGAGCCACGAGAACCAGGAAATC; TACCAGAAGGCCTTTGACCTCATTGAGCACTACTTTGGTGTAGAGGATGACGACAGCAGCCTGGCTCCTC; AGGTGGATGAGACGCAACAGCAGTTCATCTTCCAGCAGCCTGAGGCCCCCATGGAGGGCTTCCAGCTATA; A; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008468
• Insert Size: 1611 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008468.4, NP_032494.3
• RefSeq Size: 5711 bp
• RefSeq ORF: 1611 bp
• Locus ID: 16650
• UniProt ID: O35345
• Cytogenetics: 4 D2.2
• Summary: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.[UniProtKB/Swiss-Prot Function]